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Plasmid#154172Purposeexpresses squirrel monkey CHMP3 in mammalian cellsDepositorInsertCHMP3
UseTagsHAExpressionMammalianMutationPromoterEF1-alphaAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-control-APEX
Plasmid#73187PurposeExpresses "control"-APEX (cytosolic) in mammalian cellsDepositorInsertcontrol-APEX (Nphp3 Glycine max, Mouse)
UseTagsinternal GFP tagExpressionMammalianMutationchanged glycine 2 to alaninePromoterEF1alphaAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pActinin-LSSmOrange
Plasmid#37139DepositorInsertLSSmOrange
UseTagshuman alpha-actininExpressionMammalianMutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOr…PromoterCMVAvailable sinceJune 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
Cre Reporter
Plasmid#62732PurposeMammalian Cre recombinase reporterDepositorInsertLoxP-DsRED-STOP-LoxP-eGFP
UseCre/Lox and LentiviralTagsLoxP sitesExpressionMammalianMutationPromoterEF1-AlphaAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(EF1a)
Plasmid#172326PurposeLV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an EF1a promoter driving nuclear d2eGFP E2A HygromycinDepositorInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterEF1-alphaAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV CMV mCherry-DDX21-V5
Plasmid#175163PurposeLentiviral expression of human DDX21-V5DepositorInsertDDX21 (DDX21 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
TMD24
Plasmid#163678PurposeExpresses a ST6GAL1 mutant with 24 amino acids in its TMD in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
UseTagsGFPExpressionMammalianMutationFor this partial length ST chimera, two restricti…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLSSmKate1-Tubulin-C1
Plasmid#31902DepositorInsertalpha-tubulin
UseTagsExpressionMammalianMutationK69Y/P131T/S148G/M167E/T183S/M196V compared to mK…PromoterCMVAvailable sinceSept. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pActinin-LSSmKate1-N1
Plasmid#31906DepositorInsertalpha-actinin LSS-mKate1
UseTagsExpressionMammalianMutationK69Y/P131T/S148G/M167E/T183S/M196V compared to mK…PromoterAvailable sinceSept. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TNF
Plasmid#67062PurposeStandard for cell-free expression benchmarking.DepositorInsertTNF (TNF Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVET-tTR-KRAB
Plasmid#11644PurposeTet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInserthEF-1alpha, GFP, tTR-KRAB, Tet-on
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(EF1a)-TTN
Plasmid#172327PurposeLV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an EF1a promoter driving nuclear d2eGFP E2A HygromycinDepositorInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterEF1-alphaAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7 Alk.1 shRNA
Plasmid#59296PurposeshRNA to mouse AlkDepositorInsertAlk.1 shRNA
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromotermouse U6Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7 Alk.2 shRNA
Plasmid#59297PurposeshRNA to mouse AlkDepositorInsertAlk.2 shRNA
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromotermouse U6Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUpstream2Lox
Plasmid#164573PurposeIntegration of 2 LoxN sites in the genome of P. bergheiDepositorInsertsUseCre/LoxTagsExpressionMutationPromoterBidirectional PbEF1alpha promoter (PBANKA_1133300…Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TCF3
Plasmid#67110PurposeStandard for cell-free expression benchmarking.DepositorInsertTCF3 (TCF3 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationTruncated isoformPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPU-Empty-miR30-L1221
Plasmid#132940PurposeAll-in-one control rescue-control shRNA knockdown vectorDepositorInsertGFP
UseLentiviral and RNAiTagsHAExpressionMammalianMutationPromoterSFFVAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_Hinge Notch SNIPR (LaG17)
Plasmid#188380PurposeLentiviral vector - constitutive expression of an anti-GFP SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiGFP_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
HSC1-GiP
Plasmid#58254PurposeThis self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassetteDepositorInsertEnhanced Green Fluorescence Protein, Puromycin resistance gene
UseRetroviralTagsExpressionMammalianMutationPromoterHuman EF1-alphaAvailable sinceOct. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CSNK2A1
Plasmid#67085PurposeStandard for cell-free expression benchmarking.DepositorInsertCSNK2A1 (CSNK2A1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only