Addgene

We narrowed to 11,048 results for: cel.2

Showing: 8961 - 8980 of 11048 results

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MTK1_013

Plasmid

#123676

Purpose

Encodes ConL5 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L5-2xHS4

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCI Flag Smad3 NL* EPSM

Plasmid

#27005

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa 1-203; T179V

Promoter

Available since

Jan. 28, 2011

Availability

Academic Institutions and Nonprofits only
pCI Flag Smad3 NL* WT

Plasmid

#27042

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa 1-203

Promoter

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
pCI Flag Smad3 NL AAGY

Plasmid

#27041

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa 1-226; P181A P182A

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pGEX6-Smad3 EPSM

Plasmid

#27222

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

T179V S204A S208A S213A

Promoter

Available since

Dec. 21, 2010

Availability

Academic Institutions and Nonprofits only
pGEX6-Smad3 AAGY

Plasmid

#27013

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

P181A P182A

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pAc5.1A-Dm-mir-6-1,2,3

Plasmid

#22789

Depositor

Elisa Izaurralde

Article

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

micro RNA 6-1, micro RNA 6-2, micro RNA 6-3 (mir-6-1 primary transcript, Fly)

Use

Drosophila expression

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pCRISPR-HOT_tdTomato

Plasmid

#138567

Purpose

Universal Cleavable Donor Plasmid for tagging with tdTomato using NHEJ

Depositor

Hans Clevers

Article

Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

Insert

tdTomato

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pfa6a PhoCl-TsCC(B)::KANMX

Plasmid

#185906

Purpose

Plasmid for cloning PhoCl fused to TsCC(B) into yeast.

Depositor

Matthew Good

Article

Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

Insert

PhoCl-TsCC(B)

Use

Tags

PhoCl fluorophore with C-terminal TsCC(B)

Expression

Yeast

Mutation

Promoter

SP6

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_Olig2

Plasmid

#64842

Purpose

A lentiviral vector for the expression of mouse Olig2 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Olig2 (Olig2 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV3flag8HOIL-1L

Plasmid

#50016

Purpose

Mammalian expression of human HOIL-1L (RBCK1) with a c-terminal 3xFLAG tag, and hygromycin selection.

Depositor

Martin Dorf

Article

Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

Insert

RanBP-type and C3HC4-type zinc finger containing 1 HOIL-1L (RBCK1 Human)

Use

Tags

3 x FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-ING2

Plasmid

#109502

Purpose

Muscle specific zebrafish expression of mKate2 fused to 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5P

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-mKate2-ING2

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_Nkx2.2

Plasmid

#64840

Purpose

A lentiviral vector for the expression of mouse Nkx2.2 under doxycyline control.

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Nkx2.2 (Nkx2-2 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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pFU-GW-sMYH6-mNeon-TNNT2-mScarlet

Plasmid

#170712

Purpose

dual fluorescent reporter for MYH6 (short) and TNNT2 promoters

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Inserts

mNeonGreen
mScarlet

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TNNT2 and aMHC

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSIN-Skil-gRNA2

Plasmid

#180371

Purpose

targeting mouse Skil/SnoN gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Skil targeting gRNA (Skil Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pYLL201

Plasmid

#53454

Purpose

Expresses HA-tagged mouse DNA topoisomerase IIbeta (full length cDNA, in pCS2+ backbone)

Depositor

Leroy Liu, Lisa Lyu

Article

Ban et al Mol Cell Biol. 2013 Oct;33(20):4008-16.

Insert

HA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) (Top2b Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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FUGW-eGFP-ZEB1 3'UTR

Plasmid

#81018

Purpose

FUGW lentiviral vector with constitutively expressed eGFP regulated by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor reported in manuscript

Depositor

Jeffrey Rosen

Article

Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Insert

Zeb1 3'UTR (UTS2R Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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lenti-25xERSE2-minP-EGFP

Plasmid

#159667

Purpose

EGFP reporter plasmid containing 25 tandem repeats of the ER stress response element-2 (ERSE2)

Depositor

Seiichi Oyadomari

Article

Kitakaze et al Elife. 2019 Dec 17;8. pii: 43302. doi: 1

Insert

25xERSE2-minP-EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 5, 2021

Availability

Academic Institutions and Nonprofits only

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Myo1E-pEGFP

Plasmid

#124658

Purpose

Mouse myosin-1e fused with EGFP

Depositor

Cheng-han Yu

Article

Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

Insert

Myo1e (Myo1e Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pEXL flag Smad3

Plasmid

#10920

Depositor

Bob Weinberg

Article

Liu et al Proc Natl Acad Sci U S A. 1997 Sep 30. 9

Insert

Smad3 (SMAD3 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only