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Plasmid#133123PurposeLevel 0 PU module synthesised as a fragment (pL0M-PU-PsNifH). Synthetic consensus promoter to drive nodule-specific expression in biovars and strains of R. leguminosarum.DepositorInsertPsNifH Synthetic consensus nifH promoter
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
PD-L1-miSFIT-12-C
Plasmid#124687PurposeTuning expression of PD-1DepositorInsertPD-L1 (CD274 Human)
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMflPT-o4
Plasmid#101315PurposeContains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and pac resistance genes, origin of transfer of RP4 plasmid.DepositorInsertsoriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
pac resistance gene
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMflST-o4
Plasmid#101316PurposeContains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and aadA1 resistance genes, origin of transfer of RP4 plasmid.DepositorInsertsoriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
aadA1 resistance gene
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX p55g(NC)-SH2
Plasmid#46462DepositorInsertp55g(NC) (PIK3R3 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 56-461PromoterTacAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Ptk70-SH2
Plasmid#46473DepositorInsertPtk70 (Srms Mouse)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 123-236PromoterTacAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Bks-SH2
Plasmid#46406DepositorInsertBks (STAP2 Human)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 141-266PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Slnk-SH2
Plasmid#46502DepositorInsertSlnk (Sh2d6 Mouse)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 199-323PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Sck-SH2
Plasmid#46478DepositorInsertSck/ShcB (Shc2 Mouse)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 464-573PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Socs7-SH2
Plasmid#46509DepositorInsertSocs7 (SOCS4 Human)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 280-386PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Nsp3-SH2
Plasmid#46461DepositorInsertNsp3 (SH2D3C Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 212-330PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
OVA-miSFIT-1xPerfect
Plasmid#124674PurposeTuning expression of cytosolic Ovalbumin coupled to EGFPDepositorInsertCyto-Ovalbumin
UseTagsExpressionMammalianMutationTruncated OVA for cytoplasmic expressionPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cerulean(1-158) in pcDNAI/Amp
Plasmid#60897PurposeThis vector attaches Cerulean(1-158) to the N-terminus of a protein.DepositorInsertCerulean(1-158)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWZLneo Flag hDPY30
Plasmid#15555DepositorInsertDPY30 (DPY30 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1‐Afu rnhB
Plasmid#108694PurposeFor expression in E coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HIIDepositorInsertrnhB (AF_RS03165 Archaeoglobus fulgidus)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT-DEST EVC2dW-TEV-YFP-Flag
Plasmid#41006DepositorInsertEllis van Creveld syndrome 2 (EVC2 Mouse)
UseTagsTEV-YFP-FlagExpressionMammalianMutationdeleted amino acids 1177-1220PromoterAvailable sinceNov. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDECKO-mCherry MALAT1_Exon.2
Plasmid#78541PurposeExperimental plasmidDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMammalianMutationPromoterU6 (for expressing sgRNA) and U6 promoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDECKO-mCherry MALAT1_Exon.4
Plasmid#78543PurposeExperimental plasmidDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMammalianMutationPromoterU6 and U6 (for expressing sgRNA)Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-LKB1(S431A)
Plasmid#84641PurposemCherry-tagged, LKB1(S431A); lacks C-terminal PKA phosphorylation siteDepositorInsertmCherry-LBK1(S431A) (STK11 Human, Synthetic)
UseTags6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …ExpressionMammalianMutationPKA phosphorylation site Serine 428 mutated to Al…PromoterCMVAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSG5-FLAG-CaMKK alpha with CaMKKbeta RP domain
Plasmid#33331DepositorInsertCalcium/Calmodulin dependent protein kinase kinase alpha (Camkk1 Rat)
UseTagsFLAGExpressionMammalianMutationCaMKKalpha RP domain (167-189) was replaced with …PromoterSV40Available sinceApril 10, 2012AvailabilityAcademic Institutions and Nonprofits only