Skip to main content
Addgene
Showing: 8941 - 8960 of 51967 results

  1. pUC19-B5JU35

    Plasmid
    #103087
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    B5JU35(Cas9 coding gene from gamma proteobacterium HTCC5015)
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103087-plasmid-map-sequence-id-201737

    Enlarge

  2. pUC19-B5XLC1

    Plasmid
    #103088
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    B5XLC1(Cas9 coding gene from Streptococcus pyogenes serotype M49 (strain NZ131))
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103088-plasmid-map-sequence-id-201738

    Enlarge

  3. pUC19-C4ZA16

    Plasmid
    #103090
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    C4ZA16(Cas9 coding gene from Eubacterium rectale (strain ATCC 33656 / VPI 0990))
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103090-plasmid-map-sequence-id-201740

    Enlarge

  4. KS6A1_HUMAN_D1

    Plasmid
    #79720
    Purpose
    This plasmid encodes the kinase domain of KS6A1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    John Chodera, Nicholas Levinson, Markus Seeliger
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    KS6A1 (RPS6KA1 Human)
    Use
    Tags
    His10-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  5. KC1G3_HUMAN_D0

    Plasmid
    #79685
    Purpose
    This plasmid encodes the kinase domain of KC1G3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    John Chodera, Nicholas Levinson, Markus Seeliger
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    KC1G3 (CSNK1G3 Human)
    Use
    Tags
    His10-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. pMuLE ENTR U6 n.s shRNA L1-L4

    Plasmid
    #62136
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Insert
    non-silencing (scrambled) shRNA
    Use
    RNAi; Mule gateway entry vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62136-plasmid-map-sequence-id-101420

    Enlarge

  9. pMuLE ENTR U6 n.s shRNA R4-R3

    Plasmid
    #62137
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Insert
    non-silencing (scrambled) shRNA
    Use
    RNAi; Mule gateway entry vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62137-plasmid-map-sequence-id-112694

    Enlarge

  10. pMuLE ENTR U6 n.s shRNA L5-L2

    Plasmid
    #62139
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Insert
    non-silencing (scrambled) shRNA
    Use
    RNAi; Mule gateway entry vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62139-plasmid-map-sequence-id-112696

    Enlarge

  11. pMuLE ENTR 7SK-miR-30 R4-R3

    Plasmid
    #62121
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    RNAi; Mule gateway entry vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62121-plasmid-map-sequence-id-101405

    Enlarge

  12. pMuLE ENTR 7SK-miR-30 L1-R5

    Plasmid
    #62118
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    RNAi; Mule gateway entry vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. pCLX-PGK-hTERT

    Plasmid
    #114315
    Purpose
    Constitutive expression of the hTert protein
    Depositor
    Patrick Salmon
    Insert
    hTERT (TERT Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114315-plasmid-map-sequence-id-218852

    Enlarge

  16. pLenti-sgRNA

    Plasmid
    #71409
    Purpose
    There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI
    Depositor
    Eric Lander, David Sabatini
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71409-plasmid-map-sequence-id-322475

    Enlarge

  17. pKD3_mCherry

    Plasmid
    #187385
    Purpose
    To chromosomally tag genes of interest with mCherry. FRT-mCherry-cat-FRT
    Depositor
    Roi Avraham
    Insert
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    187385-plasmid-map-sequence-id-371123

    Enlarge

  18. pLenti Lifeact-mRuby2 PuroR

    Plasmid
    #84386
    Purpose
    Lentiviral expression of mRuby2-tagged Lifeact with Puromycin selection in cells including neurons
    Depositor
    Ghassan Mouneimne
    Insert
    Lifeact
    Use
    Lentiviral
    Tags
    mRuby2
    Expression
    Mutation
    Promoter
    CMV immediate early
    Available since
    Jan. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    84386-plasmid-map-sequence-id-272895

    Enlarge

  19. pSm-Nuclease

    Plasmid
    #132431
    Purpose
    Expresses Serratia marcescens nuclease in E. coli using T7 promoter
    Depositor
    Mark Arbing
    Insert
    Serratia marcescens nuclease (codon optimized)
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    132431-plasmid-map-sequence-id-258770

    Enlarge

  20. pAK_EF1a_CasRx_Hygro

    Plasmid
    #134840
    Purpose
    Lentiviral expression of CasRx T2A Hygromycin
    Depositor
    Michael Yaffe
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Tags
    T2A Hygromycin
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    134840-plasmid-map-sequence-id-266452

    Enlarge

Showing: 8941 - 8960 of 51967 results