Showing: 8941 - 8960 of 145697 results
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Plasmid#84636PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub(TA)-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ABKAR-Kras
Plasmid#84637PurposeUnimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targetedDepositorInsertCerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras
UseTags6xHis, C-terminal targeting sequence from Kras, C…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub-YPet-Kras
Plasmid#84638PurposeEncodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub-YPet-Kras
UseTags6xHis, C-terminal targeting sequence from Kras, T…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-LKB1(WT)
Plasmid#84639PurposemCherry-tagged, wild-type LKB1DepositorInsertmCherry-LBK1 (STK11 Synthetic, Human)
UseTags6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-LKB1(KD)
Plasmid#84640PurposemCherry-tagged, LKB1(K78I); kinase-dead mutantDepositorInsertmCherry-LBK1(K78I) (STK11 Synthetic, Human)
UseTags6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …ExpressionMammalianMutationLysine 78 mutated to Isoleucine; kinase deadPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-LKB1(S431A)
Plasmid#84641PurposemCherry-tagged, LKB1(S431A); lacks C-terminal PKA phosphorylation siteDepositorInsertmCherry-LBK1(S431A) (STK11 Synthetic, Human)
UseTags6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …ExpressionMammalianMutationPKA phosphorylation site Serine 428 mutated to Al…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSLIK DN Rac1
Plasmid#84642PurposeLentiviral expression of doxycycline-inducible dominant negative Rac1 and co-expression of VenusDepositorInsertRac1 (RAC1 Human)
UseLentiviralTagsExpressionMammalianMutationDominant NegativePromoterAvailabilityAcademic Institutions and Nonprofits only -
pCuo CA Rac1
Plasmid#84643Purposeconstitutively active Rac1 under cumate-inducible promoter in Lentiviral vector. Also expresses RFP and puromycin resistance.DepositorInsertRac1 (RAC1 Human)
UseLentiviralTagsExpressionMammalianMutationConstitutively Active, Q61LPromotercumate-inducible promoterAvailabilityAcademic Institutions and Nonprofits only -
pCuo DN Rac1
Plasmid#84644Purposedominant negative Rac1 under cumate-inducible promoter in Lentiviral vector. Also expresses RFP and puromycin resistance.DepositorInsertRac1 (RAC1 Human)
UseLentiviralTagsExpressionMammalianMutationDominant NegativePromotercumate-inducible promoterAvailabilityAcademic Institutions and Nonprofits only -
pSLIK DN RhoA
Plasmid#84646PurposeLentiviral expression of doxycycline-inducible dominant negative RhoA and co-expression of VenusDepositorInsertRhoA (RHOA Human)
UseLentiviralTagsMycExpressionMammalianMutationDominant NegativePromoterAvailabilityAcademic Institutions and Nonprofits only -
pSLIK CA MLCK
Plasmid#84647PurposeLentiviral expression of doxycycline-inducible constitutively active MLCK and co-expression of VenusDepositorInsertMLCK (MYLK Human)
UseLentiviralTagsExpressionMammalianMutationConstitutive ActivePromoterAvailabilityAcademic Institutions and Nonprofits only -
pSLIK CA ROCK2
Plasmid#84649PurposeLentiviral expression of doxycycline-inducible constitutively active ROCK2 and co-expression of VenusDepositorInsertROCK2 (ROCK2 Bovine)
UseLentiviralTagsExpressionMammalianMutationConstitutive Active (please see depositor comment…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pRARE-MBP-DEST
Plasmid#84650PurposeGateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.DepositorTypeEmpty backboneUseTagsMBPExpressionBacterialMutationPromotertacAvailabilityAcademic Institutions and Nonprofits only -
pET32a-DEST
Plasmid#84652PurposeE. coli expression vector derived from pET32 with Gateway cassette.DepositorTypeEmpty backboneUseTagsHis, S tag, and Trx TagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pLCNICK
Plasmid#84653PurposeGenome editing for Lactobacillus casei Lc2WDepositorInsertsCas9 nickase
sgRNA
homology arms of LC2W_2179
UseCRISPRTagsExpressionMutationD10APromoterAvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-Laforin Myc
Plasmid#84656Purposemammalian expression and/or retrovirus preparationDepositorArticleInsertLaforin (EPM2A Human)
UseRetroviralTagsMycExpressionMammalianMutationPromoter5'LTRAvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-Laforin Myc (C266S,C278S)
Plasmid#84657Purposemammalian expression and/or retrovirus preparationDepositorArticleInsertLaforin (EPM2A Human)
UseRetroviralTagsMycExpressionMammalianMutationPromoter5'LTRAvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-Laforin Myc 241X
Plasmid#84658Purposemammalian expression and/or retrovirus preparationDepositorArticleInsertLaforin (EPM2A Human)
UseRetroviralTagsMycExpressionMammalianMutationContains residues 1-240 onlyPromoter5'LTRAvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-Laforin Myc 2CS_new (C278S, C329S)
Plasmid#84659Purposemammalian expression and/or retrovirus preparationDepositorArticleInsertLaforin (EPM2A Human)
UseRetroviralTagsMycExpressionMammalianMutationPromoter5'LTRAvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-Laforin Myc 2KA (K87A, K320A)
Plasmid#84660Purposemammalian expression and/or retrovirus preparationDepositorArticleInsertLaforin (EPM2A Human)
UseRetroviralTagsMycExpressionMammalianMutationPromoter5'LTRAvailabilityAcademic Institutions and Nonprofits only
Showing: 8941 - 8960 of 145697 results