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Showing: 8921 - 8940 of 14429 results

  3. pCRI003-pGEM-LS-Bar-PgpdA-dSpCas9-VPR-TtrpC-LS

    Plasmid
    #140196
    Purpose
    Chromosomal integration of PgpdA-dSpCas9-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi vector.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Inserts
    dSpCas9-VPR
    Bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    Use
    CRISPR and Synthetic Biology; Fungal genome integ…
    Tags
    VPR (VP64-p65-Rta) , NLS
    Expression
    Mutation
    Promoter
    PgpdA and PtrpC
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140196-plasmid-map-sequence-id-309706

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  7. pCri-H6-TEV-GRAB

    Plasmid
    #128498
    Purpose
    Expresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV.
    Depositor
    F Xavier Gomis-Ruth
    Article
    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.
    Insert
    GRAB (Streptococcus pyogenes serotype M1) (grab Streptococcus pyogenes serotype M1)
    Use
    Tags
    His tag (6x)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    128498-plasmid-map-sequence-id-251962

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  18. pEN_TT 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant

    Plasmid
    #72924
    Purpose
    Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human S37A-GATA6
    Depositor
    Kevin Janes
    Article
    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:
    Insert
    GATA Binding Protein 6 (GATA6 Human)
    Use
    Entry vector for gateway cloning
    Tags
    3XAU1 and 3XFLAG
    Expression
    Bacterial
    Mutation
    Serine#37 changed to Alanine;Silent mutations mad…
    Promoter
    TRE tight
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72924-plasmid-map-sequence-id-138470

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  19. pSLIKneo 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant

    Plasmid
    #72617
    Purpose
    Expresses an inducible and RNAi resistant, N-terminally 3X-FLAG tagged, C-terminally 3X-AU1 tagged S37AGATA6
    Depositor
    Kevin Janes
    Article
    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:
    Insert
    GATA Binding Protein 6 (GATA6 Human)
    Use
    Lentiviral
    Tags
    3XAU1 and 3XFLAG
    Expression
    Mammalian
    Mutation
    Serine#37 changed to Alanine;Silent mutations mad…
    Promoter
    TRE tight
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72617-plasmid-map-sequence-id-138346

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  20. pBabe 3XFLAG-S/T3A-GATA6-3XAU1 puro

    Plasmid
    #72611
    Purpose
    For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with triple alanine mutations on aminoacids#33,#34 and #37
    Depositor
    Kevin Janes
    Article
    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:
    Insert
    GATA binding protein 6 (GATA6 Human)
    Use
    Retroviral
    Tags
    3XAU1 and 3XFLAG
    Expression
    Mammalian
    Mutation
    changed serine33, threonine34 and serine37 to ala…
    Promoter
    Available since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 8921 - 8940 of 14429 results