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Plasmid#46752PurposeRetroviral vector that expresses catalytically inactive form of Flag-tagged human USP7DepositorInsertUSP7 (USP7 Human)
UseRetroviralTagsFlagExpressionMammalianMutationC223S--catalytically inactivePromoterCMVAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEY52
Plasmid#191055Purposeodr-1 fluorescent neural reporter driving nuclear mNeptune2.5 and mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertodr-1 (odr-1 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDest-Sumo
Plasmid#106980PurposeLR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tagDepositorTypeEmpty backboneUseTagsHis8-SumoExpressionBacterialMutationPromoterT7Available sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-pDUXCca
Plasmid#198236PurposeLentivector with tet-inducible porcine DUXC (codon altered)DepositorInsertporcine DUXC (codon altered)
UseLentiviralTagsExpressionMammalianMutationcodon alteredPromoterTREAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
gRNA-trp-HYB
Plasmid#64331Purposetrp1 deletion gRNA cassette carried by pRS42HDepositorInsertgBlock product of trp1 deletion gRNA cassette
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag HBx
Plasmid#42596DepositorInsertHepatitis B virus protein X
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMA_Int
Plasmid#110096PurposeE. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.DepositorInsertmycophage integrase
UseRequired for transformation of attb integrating v…TagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody
Plasmid#138133PurposeExpresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.DepositorInsertEGFP-anti-rabbit-IgG(Fc)-nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR tdTomato L5-L2
Plasmid#62185PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing tdTomato. Compatible with MultiSite Gateway cloningDepositorInserttdTomato
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1
Plasmid#41605DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutationPromoterAvailable sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
SPLICS Mt-ER- Po Short P2A
Plasmid#164118PurposeDetect the short Peroxisomes-mitochondria-Endoplasmic reticulum contactDepositorInsertSplit GFP and YFP Mt-ER- Po Short P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-hsf-1-sgRNA-B
Plasmid#177787PurposeOverexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)DepositorInserthsf-1 (hsf-1 Nematode)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
SadCas9 KRAB
Plasmid#188504PurposeExpresses FLAG tagged SadCas9 KRABDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A/N580APromoterEF1aAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-NEMOb
Plasmid#189934PurposeMammalian expression of calcium sensor with ultra-high dynamics and sensitivityDepositorInsertNEMOb
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
SPLICS Mt-ER- Po Long P2A
Plasmid#164117PurposeDetect the long Peroxisomes-mitochondria-Endoplasmic reticulum contactDepositorInsertSplit GFP and YFP Mt-ER- Po Long P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7C-HSPH1
Plasmid#177662PurposeRecombinant protein synthesis of human HSPH1DepositorInsertHSPH1 (HSPH1 Human)
UseTags6xHis-ThrombinExpressionBacterialMutationPromoterT7Available sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGE_En03
Plasmid#71535PurposeUsed to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorphaDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRelA'
Plasmid#175595PurposeTet inducible, KAN Resistant, non labelled synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.DepositorInsertCatalytic domain of E.coli relA gene.
UseTagsExpressionBacterialMutationOnly the catalytic domain of the enzyme is used (…PromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-Akt-STOPS
Plasmid#175009PurposePlasma membrane-targeted Akt-STOPS.DepositorInsertLyn-mCherry-Akt-STOPS
UseTagsN-terminal targeting sequence from Lyn kinase and…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMpGE_En01
Plasmid#71534PurposeUsed to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorphaDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only