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Plasmid#82469PurposeExpresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme, inactive formDepositorInsertmRNA capping enzyme (Rngtt Mouse)
UseTagsFlag and MycExpressionMammalianMutationchanged Lysine 294 to AlaninePromoterCMVAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-myc NES-mCE NLS-Flag
Plasmid#82468PurposeExpresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzymeDepositorInsertMyc-NES-mCE-Flag (Rngtt HIV, Mouse)
UseTagsFlag and MycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH_CMV_HMGA_linker_mKate2
Plasmid#167335PurposeExpresses mKate2 fused to TFAM HMGA+linker mutant in mammalian cellsDepositorInsertTFAM (TFAM Human)
UseLentiviralTagsmKate2ExpressionMammalianMutationTruncation mutant containing MTS (1-42) as well a…PromoterCMVAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
px330 CBP gRNA
Plasmid#165587Purposeinsertin of CBP degromDepositorInsertCBP (Crebbp Mouse)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub-YPet-Kras
Plasmid#84638PurposeEncodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub-YPet-Kras
UseTags6xHis, C-terminal targeting sequence from Kras, T…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV10/3xflag-Clock L57E
Plasmid#47337PurposeMutation used for co-IPsDepositorInsertClock (Clock Mouse)
UseTags3x FlagExpressionMammalianMutationL57EPromoterCMVAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV10/3xflag-Clock L74E
Plasmid#47338PurposeMutation used for co-IPsDepositorInsertClock (Clock Mouse)
UseTags3x FlagExpressionMammalianMutationL74EPromoterCMVAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NEDD9-1/1
Plasmid#91498PurposeProtein expression and purification of human SH3 domain construct NEDD9-1/1DepositorInsertNEDD9-1/1 (NEDD9 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAVsc.U6-sgGTTG.CMV/CB-Gluc
Plasmid#121512PurposeExpresses sgRNA targeting the intron in the MADM alleles (GT and TG).DepositorInsertsgGTTG
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX Tenc1-SH2
Plasmid#46524DepositorInsertTenc1 (TNS2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1114-1264PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSR322
Plasmid#125116PurposeExpression of uhpA(1-123aa)-ydfI(126-213aa), narL131 chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsuhpA(REC)-ydfI(DBD)131
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX Socs5-SH2
Plasmid#46508DepositorInsertSocs5 (SOCS5 Human)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 378-481PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDECKO-mCherry TFRC_B
Plasmid#78544PurposeExperimental plasmidDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMammalianMutationPromoterU6 and U6 (for expressing sgRNA)Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX Grap-SH2
Plasmid#46434DepositorInsertGrap (GRAP Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 51-160PromoterTacAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SRC-1/1-IS1/2
Plasmid#91349PurposeProtein expression and purification of human SH3 domain construct SRC-1/1-IS1/2DepositorInsertSRC-1/1-IS1/2 (SRC Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABEhygro-Flag-ZO-2-1st-PDZ-mut
Plasmid#27474DepositorInsertZonula Occludens 2 (TJP2 Human)
UseRetroviralTagsFlagExpressionMammalianMutationK38E, F44H, I46E and V48EPromoterAvailable sinceMarch 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Ywhag-3'UTR-MRE-3 MUT
Plasmid#61792PurposeLuciferase reporter containing the 3'UTR of mouse Ywhag with MRE-3 mutatedDepositorInsertmouse Ywhag 3'UTR with miR-200c MRE-3 mutated
UseLuciferaseTagsExpressionMutationmiR-200c MRE-3 mutatedPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLacIBD::SDRX (GB0673)
Plasmid#68183PurposeProvides the LacI Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid partDepositorInsertLacI Binding Domain fused to SRDX Transcriptional Repressor
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NOSTRIN-1/1-SV1/2
Plasmid#91323PurposeProtein expression and purification of human SH3 domain construct NOSTRIN-1/1-SV1/2DepositorInsertNOSTRIN-1/1-SV1/2 (NOSTRIN Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SH3KBP1-1/3
Plasmid#91555PurposeProtein expression and purification of human SH3 domain construct SH3KBP1-1/3DepositorInsertSH3KBP1-1/3 (SH3KBP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only