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pCEP4-myc-H2-Ld

Plasmid

#135519

Purpose

Mammalian expression of myc-tagged H2-Ld

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

H2-Ld

Use

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGB3_Omega2_Pnos:GR:LacIBD:Gal4AD:Tnos-OplacI:mini35S:RDF:Tnos (GB1678)

Plasmid

#160642

Purpose

Module for the dexamethasone -inducible expression of PhiC31 phage recombination directionality factor (RDF) gene.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

GRLacIBDGal4AD / RDF

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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PIK3CA - Exon9 E545K (pFastBac)

Plasmid

#16640

Depositor

Bert Vogelstein

Article

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA E545K (PIK3CA Human)

Use

Baculoviral

Tags

His

Expression

Mammalian

Mutation

E545K

Promoter

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
pTol2CG2-QUASM1:mScarletNLS-SV40pA

Plasmid

#155124

Purpose

Tol2 vector containing QUASM1 reporter element upstream of mScarlet-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1:mScarletNLS

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

QUASM1-E1b

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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Sha2Cas9-HF

Plasmid

#192142

Purpose

Expresses highly specific Sha2Cas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

Insert

Sha2Cas9-HF

Use

AAV

Tags

Expression

Mammalian

Mutation

Sha2Cas9-HF (R247A and N415A)

Promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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dmBACCS2VL-IRES-dOrai-IRES-mCherry

Plasmid

#72898

Purpose

Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2VL-IRES-dOrai-IRES-mCherry

Use

Tags

IRES-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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ins:BB1.0L; cryaa:RFP

Plasmid

#110283

Purpose

A zebrafish insulin promoter driving the Brainbow (BB1.0L) cassette. Contains red eye marker, and is flanked with I-SceI sites.

Depositor

Nikolay Ninov

Article

Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

Insert

Brainbow1.0L

Use

Zebrafish

Tags

Expression

Mutation

Promoter

insulin

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-TFAP4(E135A/S139A)

Plasmid

#110157

Purpose

Expression of human TFAP4 (E135A/S139A) in mammalian cells

Depositor

Daniele Guardavaccaro

Article

D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

Insert

TFAP4 (transcription factor AP-4) (TFAP4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

E135A/S139A

Promoter

CMV

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-p97-AE-mycStrep

Plasmid

#31841

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Use

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

A232E

Promoter

CMV

Available since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3 PARL-FLAG-CT S65D+T69D+S70D

Plasmid

#13617

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 65, Thr 69 and Ser70 to Asp

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pLKO.shNkx2-1

Plasmid

#32400

Depositor

Tyler Jacks, Monte Winslow

Article

Winslow et al Nature. 2011 May 5. 473(7345):101-4.

Insert

shRNA targetting Nkx2-1 (Nkx2-1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
lentiCRISPR.sgKras.9

Plasmid

#91894

Purpose

sgRNAs targeting mouse Kras. 3rd generation lentiviral backbone.

Depositor

Wen Xue

Article

Mou et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Insert

Kras sgRNA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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PIK3CA - Exon20 H1047R (pFastBac)

Plasmid

#16641

Depositor

Bert Vogelstein

Article

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA H1047R (PIK3CA Human)

Use

Baculoviral

Tags

His

Expression

Mammalian

Mutation

H1047R

Promoter

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
pGL-p21UTRm1

Plasmid

#20878

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

p21 3'UTR site 1 mutation (Cdkn1a Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Predicted miRNA binding site 1 (Position ~420) GC…

Promoter

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1-A774L

Plasmid

#174796

Purpose

Bacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-A774L (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

A774L, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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VEGF binding scFv G6

Plasmid

#111716

Purpose

Yeast Surface Display of G6 as a reference starting point for design

Depositor

Sarel Fleishman

Article

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

G6

Use

Tags

cmyc tag

Expression

Yeast

Mutation

Promoter

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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dmBACCS2NS-IRES-dOrai-IRES-mCherry

Plasmid

#72896

Purpose

Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2NS-IRES-dOrai-IRES-mCherry

Use

Tags

IRES-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-hu-c-Myc-dN2

Plasmid

#50774

Purpose

retroviral expression of human c-Myc dN2 fragment

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Jul 26. (

Insert

c-Myc -dN2 (MYC Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

N-terminal deletion, contains aa 42-439

Promoter

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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SOD1-VC

Plasmid

#89758

Purpose

expresses human SOD1 fused to the C-terminal part of Venus

Depositor

Tiago Outeiro

Article

Brasil et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

SOD1 (SOD1 Human)

Use

Tags

C-terminal part of Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pET-21a_2xNLS_FnCpf1_MBP_protein_expression

Plasmid

#120801

Purpose

Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

2xNLS_FnCpf1

Use

CRISPR

Tags

MBP and NLS

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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