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Plasmid#165588PurposeInsertion of CTCF degonDepositorInsertCTCF (CTCF Mouse)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPOP-V5 (BTB)
Plasmid#169721PurposeMammalian Expression of mouse SPOP-BTB (L186D, L190D, L193D, I217K)DepositorInsertSPOP (Spop Mouse)
UseTagsV5ExpressionMammalianMutationL186D, L190D, L193D, I217KPromoterCMVAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ETV3_pet28a
Plasmid#131646PurposeBacterial expression of ETS gene ETV3DepositorInsertETV3 (ETV3 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJZC39
Plasmid#62333PurposesgRNA + 1x PP7 with mCherry for mammalian cellsDepositorInsertssgRNA + 1x PP7
mCherry
UseLentiviralTagsExpressionMammalianMutationTargets Tet3G, sequence: GTACGTTCTCTATCACTGATAPromoterCMV and U6Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-myc NES-mCE(K294A) NLS-Flag
Plasmid#82469PurposeExpresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme, inactive formDepositorInsertmRNA capping enzyme (Rngtt Mouse)
UseTagsFlag and MycExpressionMammalianMutationchanged Lysine 294 to AlaninePromoterCMVAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-myc NES-mCE NLS-Flag
Plasmid#82468PurposeExpresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzymeDepositorInsertMyc-NES-mCE-Flag (Rngtt HIV, Mouse)
UseTagsFlag and MycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH_CMV_HMGA_linker_mKate2
Plasmid#167335PurposeExpresses mKate2 fused to TFAM HMGA+linker mutant in mammalian cellsDepositorInsertTFAM (TFAM Human)
UseLentiviralTagsmKate2ExpressionMammalianMutationTruncation mutant containing MTS (1-42) as well a…PromoterCMVAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
px330 CBP gRNA
Plasmid#165587Purposeinsertin of CBP degromDepositorInsertCBP (Crebbp Mouse)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub-YPet-Kras
Plasmid#84638PurposeEncodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub-YPet-Kras
UseTags6xHis, C-terminal targeting sequence from Kras, T…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV10/3xflag-Clock L57E
Plasmid#47337PurposeMutation used for co-IPsDepositorInsertClock (Clock Mouse)
UseTags3x FlagExpressionMammalianMutationL57EPromoterCMVAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV10/3xflag-Clock L74E
Plasmid#47338PurposeMutation used for co-IPsDepositorInsertClock (Clock Mouse)
UseTags3x FlagExpressionMammalianMutationL74EPromoterCMVAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NEDD9-1/1
Plasmid#91498PurposeProtein expression and purification of human SH3 domain construct NEDD9-1/1DepositorInsertNEDD9-1/1 (NEDD9 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAVsc.U6-sgGTTG.CMV/CB-Gluc
Plasmid#121512PurposeExpresses sgRNA targeting the intron in the MADM alleles (GT and TG).DepositorInsertsgGTTG
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR322
Plasmid#125116PurposeExpression of uhpA(1-123aa)-ydfI(126-213aa), narL131 chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsuhpA(REC)-ydfI(DBD)131
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX Tenc1-SH2
Plasmid#46524DepositorInsertTenc1 (TNS2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1114-1264PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Socs5-SH2
Plasmid#46508DepositorInsertSocs5 (SOCS5 Human)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 378-481PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDECKO-mCherry TFRC_B
Plasmid#78544PurposeExperimental plasmidDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMammalianMutationPromoterU6 and U6 (for expressing sgRNA)Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX Grap-SH2
Plasmid#46434DepositorInsertGrap (GRAP Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 51-160PromoterTacAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SRC-1/1-IS1/2
Plasmid#91349PurposeProtein expression and purification of human SH3 domain construct SRC-1/1-IS1/2DepositorInsertSRC-1/1-IS1/2 (SRC Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -