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pLenti-NG-PE2-BSD

Plasmid

#176933

Purpose

Expresses PE2-NG variant in mammalian cells

Depositor

Hyongbum Kim

Article

Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

Insert

PE2-NG variant

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCDHblast-flx-sORF-Katushka

Plasmid

#179457

Purpose

Lentiviral vector encoding a CRE inducible Katushka gene

Depositor

Ton Schumacher

Article

Bresser et al Nat Immunol. 2022 May;23(5):791-801. doi

Insert

loxP-shuffledORF-loxP-Katushka

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Grin1 KI #3

Plasmid

#139659

Purpose

Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Dcp1b

Plasmid

#153976

Purpose

Fluorescent protein fused to mRNA decapping enzyme 1b

Depositor

Gia Voeltz

Article

Lee et al Science. 2020 Jan 31;367(6477). pii: 367

Insert

mRNA recapping enzybe 1b (DCP1B Human)

Use

Tags

pAcGFP-C1

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mCherry-anti-rabbit-IgG(Fc)-nanobody

Plasmid

#138131

Purpose

Expresses GST-tagged mCherry-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mCherry-anti-rabbit-IgG(Fc)-nanobody

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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SpdCas9 KRAB

Plasmid

#188499

Purpose

Expresses FLAG tagged SpdCas9 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A/H840A

Promoter

EF1a

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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HiFi dCas9 KRAB

Plasmid

#188500

Purpose

Expresses FLAG tagged HiFi dCas9 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A/R691A/H840A

Promoter

EF1a

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pFUGW mCherry-KASH

Plasmid

#131505

Purpose

mCherry_KASH epression under hUBC promotor; serves as backbone for ORANGE lenti knock-in

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

mCherry_KASH

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hUBC

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDV24_AAV2_DART-VADAR_iRFP720-sensor

Plasmid

#200411

Purpose

AAV vector encoding a DART-VADAR sensor for iRFP720 mRNA

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

DART VADAR sensor

Use

AAV and Synthetic Biology

Tags

MCP-ADAR2dd(E488Q)-NES, TagBFP, and mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pEasyCas9

Plasmid

#184909

Purpose

Expresses the Streptococcus pyogenes Cas9 protein

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

Cas9

Use

CRISPR

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDA025

Plasmid

#131384

Purpose

ORFeus expression, mutant

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Use

Tags

ORF2-3xFlag

Expression

Mammalian

Mutation

H230A En

Promoter

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pLV U6-sgRNA UbC-tdTomato

Plasmid

#106949

Purpose

sgRNA cloning cassette using BsmBI/Esp3I enzymes with tdTomato marker

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

sgRNA BsmBI/Esp3I cloning site

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pHiUGE smFP-V5 donor ORF0

Plasmid

#200380

Purpose

Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHiUGE smFP-V5 donor ORF1

Plasmid

#200381

Purpose

Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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(294) pcDNA3.1-myc-OGA(1-400)

Plasmid

#168095

Purpose

Myc tagged truncated human OGA catalytic domain of residues 1-400

Depositor

Christina Woo

Article

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

myc-OGA(1-400)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

T7/CMV

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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YF-Cdc42(CA)

Plasmid

#20152

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FKBP-Cdc42(CA) (CDC42 Human)

Use

Tags

Expression

Mammalian

Mutation

Cdc42H was inserted into the multiple cloning sit…

Promoter

Available since

March 26, 2009

Availability

Academic Institutions and Nonprofits only

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C3 luc (T1 del)

Plasmid

#11358

Depositor

Donald McDonnell

Article

Fan et al Mol Endocrinol. 1996 Dec . 10(12):1605-1

Insert

C3 promoter (C3 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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mKO2-SLBP(18-126)

Plasmid

#83914

Purpose

Fluorescent probe for S/G2 cell cycle transition

Depositor

Michael Lin

Article

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mKO2-SLBP(18-126) (SLBP Human, Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pTS98 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-MCS

Plasmid

#199351

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP-P2A-ALFA

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-PGAL1-3HA

Plasmid

#41608

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Expression

Mutation

Promoter

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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