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Plasmid#151921PurposeMammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationd1-15 d1209-1273PromoterCMVAvailable sinceMay 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4134122
Plasmid#152107PurposeMammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;CBPExpressionMammalianMutationwtPromoterCMVAvailable sinceMay 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133241
Plasmid#152126PurposeMammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;TRXExpressionMammalianMutationwtPromoterCMVAvailable sinceMay 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAW8ENdeI-cyEGFP
Plasmid#48988Purposefor Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTagsyEGFPExpressionMutationPromoterAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-8XDSR
Plasmid#48981Purposefor Cre-lox cassette exchange of untagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMutationPromoterAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-c3HA
Plasmid#48989Purposefor Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTags3XHA tagExpressionMutationPromoterAvailable sinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-3XDSR
Plasmid#48976Purposefor Cre-lox cassette exchange of untagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMutationPromoterAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
membrane-mCherry-SpVasa
Plasmid#185586Purposeto recruit Vasa to membraneDepositorInsertVasa
UseIn vitro transcription (mrna synthesis)TagsmCherry, S-Tag and membrane targeting sequence fr…ExpressionMutationPromoterAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-SpGalpha-i
Plasmid#185568Purposeto see protein dynamicsDepositorInsertGalpha-i
UseIn vitro transcription (mrna synthesis)TagsGFP, S-TagExpressionMutationPromoterAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK006
Plasmid#176710PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor-responsive promoter SST2 (response part).DepositorArticleInsertP-SST2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK007
Plasmid#176711PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor-responsive promoter YPS1 (response part).DepositorArticleInsertP-YPS1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK016
Plasmid#176720PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Saccharomyces cerevisiae (sender part).DepositorArticleInsertmfalpha1Sc
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK027
Plasmid#176731PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Saccharomyces cerevisiae (receiver part).DepositorArticleInsertste2Sc
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK003
Plasmid#176707PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor-responsive promoter FUS3 (response part).DepositorArticleInsertP-FUS3
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_PKM-Halo
Plasmid#175321PurposeProduction of HaloTag fusion proteinDepositorInsertPKM (PKM Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDYU1B-TED
Plasmid#154299PurposeA expression vector in Dictyostelium discoideum containing the Blasticidin S resistant gene and the TED module for GFP-negative screeningDepositorTypeEmpty backboneUseDictyostelium expressionTagsExpressionMutationPromoteractin15Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4133975
Plasmid#152069PurposeMammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;TapTagExpressionMammalianMutationwtPromoterCMVAvailable sinceMay 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4134225
Plasmid#152042PurposeMammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;V5ExpressionMammalianMutationwtPromoterCMVAvailable sinceMay 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
relAE335Q_NS1
Plasmid#100917Purposeexpresses E.coli relAE335Q from the NS1 site in the S. elongatus PCC7942 genomeDepositorInsertRelA fragment from E.coli with point mutation E335Q
UseCyanobacteria cloning vectorTagsFLAGExpressionMutationE 335 converted to QPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
rpaA_NS1
Plasmid#101829Purposedrives expression of rpaA from the NS1 site in the S. elongatus PCC7942 genomeDepositorInsertRpaA with its native promoter
UseCyanobacteria cloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only