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Plasmid#71536PurposeFor CRISPR/Cas9 genome editing in Marchantia polymorphaDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only
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Pet15b-Endosomal Escape Control
Plasmid#186552PurposeNegative Control confirming the need for a CPP peptide in mediating internalizationDepositorInsert6H-ETA-GFP
UseTags6xHis and EGFPExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Internalization and Endosomal Escape
Plasmid#186551PurposeInternalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cellsDepositorInsert10R-8His-ETA-EGFP
UseTags8xHis and EGFPExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Beclin1 CC
Plasmid#24403DepositorInsertBeclin1 (BECN1 Human)
UseTagsHisExpressionBacterialMutationContains the CC domain (aa 151-242)PromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
dCas9-10xSunTag(22aa)
Plasmid#215745PurposeExpresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkersDepositorInsertdCas9-10xSunTag
UseTagsExpressionMammalianMutationPromoterchicken beta-actin promoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
hGATA-1 WT
Plasmid#118352PurposeThis plasmid expresses human GATA-1 WT.DepositorInserthGATA-1 (GATA1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationWTPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
YFP Gamma 8
Plasmid#36106DepositorInsertG protein subunit Gamma 8 (GNG8 Human)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-NEMOm
Plasmid#189930PurposeMammalian expression of calcium sensor with ultra-high dynamics and sensitivityDepositorInsertNEMOs
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-His-MBP-SCov2-nsp12
Plasmid#154759PurposeExpression of SARS-CoV-2 nsp12DepositorInsertSARS-CoV-2 nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionInsectMutationPromoterPolyhedronAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
anti-Notch2_B6-pBIOCAM5
Plasmid#39347DepositorInsertscFv-Fc-fusion specific for Notch2 (clone B6) (NOTCH2 Human)
UseTags3xFLAG, 6xHis, and human FcExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-DogTag Loop A
Plasmid#171779PurposeExpresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23DepositorInsertsfGFP-DogTag Loop A
UseTagsHis6ExpressionBacterialMutationDogTag and linkers inserted between residues Val2…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
HiFi Cas9
Plasmid#188490PurposeExpresses FLAG tagged HiFi Cas9DepositorInsertCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationR691APromoterEF1aAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046785
Plasmid#145684PurposeBacterial Expression plasmid for SARS-CoV-2 N (nucleocapsid)DepositorInsertSARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;StrepIIExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTS107 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-GFP
Plasmid#199360PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTEV-SUMOEu-EGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnn4
Plasmid#164960PurposeZebrafish kcnn4 gene CDSDepositorInsertkcnn4 (kcnn4 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b SARS-CoV PLpro
Plasmid#61693Purposeinducible expression of SARS PLpro proteaseDepositorInsertSARS-CoV PLpro
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pH6HTC_CCT2-Halo
Plasmid#175337PurposeProduction of HaloTag fusion proteinDepositorInsertCCT2 (CCT2 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_P0826-BB0323-mCherryBb
Plasmid#118244PurposeKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes a BB0323-mCherry translational fusion under the control of the bb0826 promoterDepositorInsertBB0323-mCherry translational fusion under the control of the bb0826 promoter
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Nanog-2A-mCherry
Plasmid#48680PurposeFor knock-in donor vector by CRISPR/CAS systemDepositorInsertNanog (Nanog Mouse)
UseCRISPRTagsCherryExpressionMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXs-DDX3X
Plasmid#70648PurposeExpresses WT DDX3X in mammalian cells under blasticidin selection.DepositorInsertDDX3X (DDX3X Human)
UseRetroviralTags3x FLAGExpressionMammalianMutationPromoterAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only