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Plasmid#86659PurposeLuciferase reporter containing CDK4 promoterDepositorInsertCDK4 promoter (CDK4 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationgenerated by cleavage at the KpnI site from 880 (…PromoterCDK7Available sinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
MT79
Plasmid#63112PurposeThe artificial microRNA is designed for down-regulating expression of ACD6 mRNA.DepositorInsertamiR-ACD6
UseTagsExpressionPlantMutationPromoterAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Cx43-7
Plasmid#57603PurposeLocalization: Gap Junctions, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertCx43
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMSCV-puro-mMCL1 Delta C22
Plasmid#45820DepositorInsertmMCL-1 Delta C22
UseTagsExpressionMammalianMutationC-terminal 22 amino acids are deleted from full l…PromoterAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-Delta N67-mMCL1
Plasmid#45819DepositorInsertDelta N67-mMCL-1
UseTagsExpressionMammalianMutationN-terminus 67 amino acids deleted from full lengt…PromoterAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAV10.K3.loxP
Plasmid#63202PurposepCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAV10.K5.loxP
Plasmid#63204PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAV10.HO3.loxP
Plasmid#63205PurposepCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAV10.HO6
Plasmid#63211PurposepCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into HO locus (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pOCC102-MBP-eIF4G-Sk
Plasmid#219976PurposeeIF4G-Sk protein purificationDepositorInserteIF4G-Sk
UseTags6xHis-MBPExpressionInsectMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-dCas9-KRAB-gRNA-TRE-blast
Plasmid#201152PurposeLentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.DepositorInsertsdCas9-KRAB
gRNA-TRE
UseCRISPR and LentiviralTagsHAExpressionMutationgRNA sequence: TACGTTCTCTATCACTGATPromoterAvailable sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVW296
Plasmid#139496PurposeExpression vector for the PP7-mCherry protein in S. cerevisiaeDepositorInsertpADH1 PP7∆FG-mCherry tCYC1
UseTagsExpressionYeastMutation∆FGPromoterpADH1Available sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
PE-SUMO CRYAB
Plasmid#217670PurposeFor expression of SUMO tagged human alpha B crystallinDepositorInsertCRYAB (CRYAB Human)
UseTags6XHis and Smt3ExpressionBacterialMutationPromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pyEvolvR (enCas9-PolI5M)
Plasmid#158073PurposeExpresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI5M
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP64-Lifeact-GFP
Plasmid#135601PurposeExpression of Lifeact-GFP mRNA for microinjection for visualization of actin in S. purpuratusDepositorInsertLifeact-GFP
UseIn vitro transcription (mrna synthesis)TagsEGFPExpressionMutationPromoterUnknownAvailable sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAMS366-4xCDRE-GFP-PEST
Plasmid#138658Purposedestabilized GFP reporter to monitor calcineurin activity via calcineurin-dependent response element (CDRE) in S. cerevisiaeDepositorInsert4xCDRE-CYC1(PM)-yEGFP3-PEST
UseYeast reporter plasmidTagsExpressionMutationPromoterCYC1Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4134100
Plasmid#152050PurposeMammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHis-003ExpressionMammalianMutationwtPromoterCMVAvailable sinceMay 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pH6HTC_TKT-Halo
Plasmid#175331PurposeProduction of HaloTag fusion proteinDepositorInsertTKT (TKT Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pSP64-Vasa-GFP
Plasmid#135595PurposeExpression of Vasa-GFP mRNA for microinjection for visualization of Vasa protein dynamics in S. purpuratusDepositorInsertVasa-GFP
UseIn vitro transcription (mrna synthesis)TagsEGFPExpressionMutationPromoterSP6Available sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only