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Plasmid#46415DepositorInsertchimerin (CHN1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 44-147PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBpuro - HA KLF4 FL delta K5S
Plasmid#34592DepositorInsertKLF4 (KLF4 Human)
UseRetroviralTagsHA tagExpressionMammalianMutationdeletion of K5S, corresponding to bases 1781 to 2…PromoterLTRAvailable sinceJan. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMiR-CIP2A(partial)
Plasmid#53705PurposeLuciferase reporter assay for microRNA binding on CIP2A coding sequenceDepositorInsertCIP2A (CIP2A Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceAug. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBullet-end-n
Plasmid#53073Purposedestination vector with ECFP- endosome marker (RabF2a) for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBullet-pm-n
Plasmid#53077Purposedestination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBullet-vac-n
Plasmid#53075Purposedestination vector with vacuole marker (gamma-TIP) - ECFP for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_ARPM1
Plasmid#45259DepositorInsertARPM1 (ACTRT3 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-Luc-KLF4-K5S-Mt344A
Plasmid#34601DepositorInsertKLF4 (KLF4 Human)
UseLuciferaseTagsExpressionMammalianMutationThe KLF4 K5S region was mutated at the miR-344 bi…PromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-Luc-KLF4-K5S-Mt206B
Plasmid#34600DepositorInsertKLF4 (KLF4 Human)
UseLuciferaseTagsExpressionMammalianMutationThe KLF4 K5S region was mutated at the miR-206 bi…PromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUC57 mini-ZsGreen-P2A-FLAG-HA-Regnase-1 D141N
Plasmid#222650PurposeFor in vitro transcription of mouse Zc3h12a D141N mutant tagged with FLAG-HA at N-termDepositorInsertZc3h12a (Zc3h12a Mouse)
UseIn vitro transcriptionTagsFLAG and HAExpressionMutationmutated aspartic acid 141 to asparagine to abroga…PromoterT7Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor-FMR1-CGG-BsmBI
Plasmid#222966Purpose0CGG with BsmBI site for inserting CGG repeatsDepositorInsertFMR1 homologous template (no CGG repeats) with cloning site (FMR1 Human)
UseCRISPRTagsExpressionMutationCGG repeats deleted from 5' UTR, BsmBI site …PromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor-FMR1-dCGG0
Plasmid#222965PurposeTo create 0CGG FMR1 cell lineDepositorInsertFMR1 homologous template no CGG repeats (FMR1 Human)
UseCRISPRTagsExpressionMutationCGG repeats deleted from 5' UTRPromoterAvailable sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CLIP-CD28(CD45TM)
Plasmid#223629PurposeLentiviral expression of human CD28 (CD45TM) with CLIP tag in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsCLIPExpressionMutationCD28 transmembrane domain replaced by CD45 transm…PromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CLIP-CD28(Y191F)
Plasmid#223628PurposeLentiviral expression of human CD28 (Y191F) with CLIP tag in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsCLIPExpressionMutationY191FPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CD28(CD45TM)
Plasmid#223626PurposeLentiviral expression of human CD28 (CD45TM) in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsExpressionMutationCD28 transmembrane domain replaced by CD45 transm…PromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-Spy-CD28(CD45TM)
Plasmid#223624PurposeLentiviral expression of human CD28 (CD45TM) with SpyTag003 in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsSpytag003ExpressionMutationCD28 transmembrane domain replaced by CD45 transm…PromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-Spy-CD28(Y191F)
Plasmid#223623PurposeLentiviral expression of human CD28 (Y191F) with SpyTag003 in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsSpytag003ExpressionMutationY191FPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-mGFP-Eps15DN
Plasmid#223621PurposeTransient expression of human Eps15 with mGFP tag in mammalian cellsDepositorInsertEps15 (EPS15 Human)
UseTagsmGFPExpressionMammalianMutationEps15 _ 95-295aaPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-PDL1-LgBiT
Plasmid#223619PurposeLentiviral expression of human PD-L1 with LgBiT tag in mammalian cellsDepositorInsertPD-L1 (CD274 Human)
UseLentiviral; mammalian expressionTagsLgBiTExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CD86-SmBiT
Plasmid#223618PurposeLentiviral expression of human CD86 with SmBiT tag in mammalian cellsDepositorInsertCD86 (CD86 Human)
UseLentiviral; mammalian expressionTagsSmBiTExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only