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pTK-EdnrbE2-Sox10-FLAG-tev-AVI

Plasmid

#127775

Purpose

Modified pTK plasmid containing chicken EdnrB E2 enhancer driving expression of full length Chicken Sox10 in frame with a Flag-tag and Avi-tag

Depositor

Tatjana Sauka-Spengler

Article

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Insert

sox10 (SOX10 Chicken)

Use

Enhancer reporter construct

Tags

Avi

Expression

Mutation

Promoter

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pTF279 (pFA6a-TEV-6xGly-2xStrep-HphMX)

Plasmid

#44082

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

2xStrep tag, 6xGly linker, ADH1 transcription ter…

Expression

Mutation

Promoter

Available since

April 3, 2013

Availability

Academic Institutions and Nonprofits only

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CMVTO-SEAP-26Sfur-B2AD-tevs-KKMP

Plasmid

#179636

Purpose

TEVP-inducible single TM SEAP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pTF275 (pFA6a-TEV-6xGly-2xStrep-His3MX)

Plasmid

#44070

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

2xStrep tag, 6xGly linker, ADH1 transcription ter…

Expression

Mutation

Promoter

Available since

April 2, 2013

Availability

Academic Institutions and Nonprofits only

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R701-X71-527: His6-tev-Hs.CALM1(1-80)

Plasmid

#159694

Purpose

E. coli protein expression of His6-tev-Hs.CALM1(1-80)

Depositor

Dominic Esposito

Article

Agamasu et al Biophys J. 2019 Mar 19;116(6):1049-1063.

Insert

His6-tev-Hs.CALM1(1-80) (CALM1 Human)

Use

Tags

His6-tev

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pTF267 (pFA6a-TEV-6xGly-12xHis-KanMX)

Plasmid

#44093

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

12xHis tag, 6xGly linker, ADH1 transcription term…

Expression

Mutation

Promoter

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S S730A

Plasmid

#166801

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. The S730A mutant is catalytically dead.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Tags

FLAG-TEV

Expression

Mammalian

Mutation

S730A

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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His-TEV-Aurein1.2_(+36GFP)-LPETG-His

Plasmid

#71755

Purpose

His-TEV-Aurein1.2+36GFP-LPETG-His expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

Aurein1.2_(pos36_GFP)-LPETG-His

Use

Tags

6XHis, Sortase (LPETG), and TEV Protease

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI2d10-364delta263-295-TEV-STREP

Plasmid

#171830

Purpose

Mammalian expression of human WIPI2d 10-364 delta263-295 with C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Use

Tags

Strep

Expression

Mammalian

Mutation

10-364delta263-295

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 CARD8-TEV-GFP-FLAG S297A

Plasmid

#169992

Purpose

Mammalian purification construct for CARD8 with an autoprocessing-deficient mutation

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

TEV-siriusGFP-FLAG

Expression

Mammalian

Mutation

S297A

Promoter

CMV

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pKK-BI16-ORF1-3C-FLAG_ORF2-TEV-mClover3

Plasmid

#105803

Purpose

Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C protease; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

ORF1: 3C-FLAG; ORF2: TEV-mClover3

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-AVPR2-eLOV-TEVcs-FLAG-GAL4-V5

Plasmid

#104844

Purpose

b2AR-SPARK TF component

Depositor

Alice Ting

Article

Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

AVPR2-eLOV-TEVcs-FLAG-GAL4-V5

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2018

Availability

Academic Institutions and Nonprofits only

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V2-MESA-35F-Tev-MS2-p65-HSF1

Plasmid

#84513

Purpose

MESA protease chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, and Tev protease, MS2-P65-HSF1

Depositor

Joshua Leonard

Article

Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

Inserts

V2-MESA-35F-Tev
MS2-P65-HSF1

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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p28BIOHTEV-LIC: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

Plasmid

#167848

Purpose

Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp10, nsp16

Use

Tags

6xHis and AviTag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 22, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S KK93EE

Plasmid

#166796

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. KK93EE mutant is partial loss of CARD8 binding.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Tags

FLAG-TEV

Expression

Mammalian

Mutation

KK93EE

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S E597R

Plasmid

#166800

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. E597R mutant is partial loss of CARD8 binding.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Tags

FLAG-TEV

Expression

Mammalian

Mutation

E597R

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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GST-CHMP4C (216-233) (pGEX2T(TEV))

Plasmid

#80635

Purpose

Residues 216-233 of CHMP4C; Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-202

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

CHMP4C (CHMP4C Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Residues 216-233

Promoter

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTF263 (pFA6a-TEV-6xGly-12xHis-His3MX)

Plasmid

#44069

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

12xHis tag, 6xGly linker, ADH1 transcription term…

Expression

Mutation

Promoter

Available since

April 2, 2013

Availability

Academic Institutions and Nonprofits only

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GST-CHMP4B (205-224) (pGEX2T(TEV))

Plasmid

#80634

Purpose

Residues 205-224 of CHMP4B, Bacterial Expression Vector; TEV-cleavabel GST tag; Sundquist Lab Internal ID: WISP06-201

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

CHMP4B (CHMP4B Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Residues 205-224

Promoter

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pZC05 (pFA6a-TEV-6xGly-PrtA-KanMX)

Plasmid

#44096

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

6xGly linker, ADH1 transcription terminator, KanM…

Expression

Mutation

Promoter

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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