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Showing: 861 - 874 of 874 results

  2. tRNA-gRNA position [M1_n-1] (GB1210)

    Plasmid
    #75411
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_n-1]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (2-part multiplexing)
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    tRNA-gRNA position [M1_n-1]
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75411-plasmid-map-sequence-id-211947

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  4. pVD1 M3_3-pTRNA-scf 2.1 (GB2075)

    Plasmid
    #160560
    Purpose
    tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M3_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter
    Depositor
    Diego Orzaez
    Article
    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-
    Insert
    M3_3-pTRNA-scf 2.1
    Use
    CRISPR
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160560-plasmid-map-sequence-id-319297

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  5. pVD1 M1_3-pTRNA-scf 2.1 (GB2073)

    Plasmid
    #160558
    Purpose
    tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M1_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.
    Depositor
    Diego Orzaez
    Article
    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-
    Insert
    M1_3-pTRNA-scf 2.1
    Use
    CRISPR
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160558-plasmid-map-sequence-id-319296

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  7. pVD1 M2_3-pTRNA-scf 2.1 (GB2074)

    Plasmid
    #160559
    Purpose
    tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M2_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter
    Depositor
    Diego Orzaez
    Article
    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-
    Insert
    M2_3-pTRNA-scf 2.1
    Use
    CRISPR
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Feb. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    160559-plasmid-map-sequence-id-319295

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  8. tRNA-gRNA position [M1_2] (GB1209)

    Plasmid
    #75410
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_2]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (3-part multiplexing)
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    tRNA-gRNA position [M1_2]
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75410-plasmid-map-sequence-id-146027

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  9. pdCas9 (GB1079)

    Plasmid
    #75399
    Purpose
    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusions
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75399-plasmid-map-sequence-id-151817

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  10. pVD1 Multiplexing Edit En-1 (GB2242)

    Plasmid
    #160564
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the position [5_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d
    Insert
    Multiplexing Edit (En-1)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160564-plasmid-map-sequence-id-319675

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  12. pVD1 tRNA-gRNA E4-En-1 (GB2243)

    Plasmid
    #160565
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the position [4_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d
    Insert
    tRNA-gRNA position E4-En-1 (Multiplexing Edit)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160565-plasmid-map-sequence-id-319679

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  13. pVD1 Multiplexing Dicot Edit E1_NTERM (GB2245)

    Plasmid
    #160567
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for position [D1_n] of a monocistronic tRNA-gRNA
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d
    Insert
    Multiplexing Dicot Edit E1_NTERM (Multiplexing Dicot Edit)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160567-plasmid-map-sequence-id-319681

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  14. pVD1 tRNA-gRNA position E3 (GB2240)

    Plasmid
    #160562
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the third position (positon [D3_4]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d
    Insert
    tRNA-gRNA position E3 (Multiplexing Edit)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160562-plasmid-map-sequence-id-319301

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Showing: 861 - 874 of 874 results