Skip to main content
Addgene
Showing: 861 - 880 of 5015 results

  2. pY116 (pcDNA3.1-huMb2Cpf1)

    Plasmid
    #92292
    Purpose
    Expression of humanized Mb2Cpf1
    Depositor
    Feng Zhang
    Insert
    huMb2Cpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    92292-plasmid-map-sequence-id-181491

    Enlarge

  4. pcDNA3.1_mGFP-FGFR3-K508M

    Plasmid
    #191753
    Purpose
    Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K508M leads to inactive receptor (target mutation: c.1523A>T; silent mutation added on purpose: c.1515A>T)
    Depositor
    Irene Tiemann-Boege
    Article
    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi
    Insert
    mGFP-FGFR3IIIc
    Use
    Tags
    mGFP
    Expression
    Mammalian
    Mutation
    c.1523A>T (p.K508M) + silent mutation c.1515A&…
    Promoter
    Available since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    191753-plasmid-map-sequence-id-382274

    Enlarge

  5. pcDNA3.1-MS2-HA-HsNeuroguidin_AA

    Plasmid
    #148237
    Purpose
    Mammalian Expression of HsNeuroguidin
    Depositor
    Elisa Izaurralde
    Insert
    HsNeuroguidin (NGDN Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    two silent mutations compared to the sequence giv…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148237-plasmid-map-sequence-id-429663

    Enlarge

  10. pcDNA3.1 myc-PITPalpha

    Plasmid
    #58277
    Purpose
    mammalian expression of human PITP alpha
    Depositor
    Shamshad Cockcroft
    Insert
    PITP alpha (PITPNA Human)
    Use
    Tags
    myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pcDNA3.1(+)-FluoSTEP-AKAR

    Plasmid
    #181844
    Purpose
    Genetically encoded FRET-based sensor for monitoring PKA activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.
    Depositor
    Jin Zhang
    Article
    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.
    Insert
    FluoSTEP-AKAR
    Use
    Tags
    mRuby2 and sfGFP(1-10)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181844-plasmid-map-sequence-id-364069

    Enlarge

  19. pcDNA3.1-Renilla-ccdB

    Plasmid
    #87068
    Purpose
    Mammalian Gateway-compatible expression vector backbone with an N-terminal Renilla luciferase
    Depositor
    Mikko Taipale
    Type
    Empty backbone
    Use
    Tags
    Renilla luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    87068-plasmid-map-sequence-id-173525

    Enlarge

Showing: 861 - 880 of 5015 results