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pETM6-M.accABCD

Plasmid

#66865

Purpose

Encodes accA, accB, accC, accD in monocistronic configuration.

Depositor

Mattheos Koffas

Article

Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

Inserts

accA
accB
accC
accD

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pDpsTE-Ypet

Plasmid

#98277

Purpose

mammalian expression of DpsTE-Ypet

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

Dps

Use

Tags

Ypet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pETM6-vioABECD

Plasmid

#66535

Purpose

pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with consensus promoter

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Inserts

VioA
VioB
VioE
VioC
VioD

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Consensus T7

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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pETM41-EcPPK

Plasmid

#38334

Depositor

Florian Freimoser

Insert

E. coli polyphosphate kinase (ppk Escherichia coli)

Use

Tags

6xHIS, MBP, and TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
pET15b-Opt12

Plasmid

#61697

Purpose

Expresses PTDH mutant Opt12

Depositor

Huimin Zhao

Article

McLachlan et al Biotechnol Bioeng. 2008 Feb 1;99(2):268-

Insert

Opt12

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 3, 2015

Availability

Academic Institutions and Nonprofits only
pET21a AHU

Plasmid

#26640

Depositor

Phoebe Rice

Article

Swinger et al EMBO J. 2003 Jul 15. 22(14):3749-60.

Insert

AHU

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only

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PCx35IL-pET42

Plasmid

#42916

Depositor

John O'Brien

Article

Burr et al Biochem Biophys Res Commun. 2005 Oct 7;3

Insert

Connexin 35 cytoplasmic loop

Use

Tags

6x His, GST, and S-tag

Expression

Bacterial

Mutation

cytoplasmic loop is aa 101-179

Promoter

T7

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pET28a-mCherryGGBsMTS

Plasmid

#133625

Purpose

Expression vector for mCherry fused to a membrane targeting sequence (MTS) of the B.subtilis MinD (C-terminal fusion) and an N-terminal His-tag for purification.

Depositor

Petra Schwille

Article

Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

Insert

mCherryGGBsMTS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET19b+H2B

Plasmid

#157809

Purpose

Expression of human histone H2B

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H2B

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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PET-3a-chpBK

Plasmid

#155201

Purpose

To express recombinant ChpBK protein

Depositor

Guanzheng Luo

Article

Zhang et al Sci Adv. 2019 Jul 3;5(7):eaax0250. doi:

Inserts

chpS-chpBK-6×His-Tag
GyrA-CBD-10xHis-Tag

Use

Tags

10xHis and 6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b-PU.1-ETS

Plasmid

#85734

Purpose

Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272

Depositor

Gregory Poon

Article

Wang et al J Biol Chem. 2014 Aug 1;289(31):21605-16

Insert

ETS domain of murine PU.1 (residues 167-272) (Spi1 Human, Mouse)

Use

Tags

6xHis following thrombin cleavage site LVPR|G

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a Gx-NL3

Plasmid

#169108

Purpose

Expresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

Depositor

Maarten Merkx

Article

Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

Insert

protein G - NanoLuc - NanoLuc - NanoLuc

Use

Tags

his-tag and strep-tag

Expression

Bacterial

Mutation

Ala 24 stop

Promoter

T7

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pFS_0354_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2_RC

Plasmid

#116966

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pET19b Adrm1

Plasmid

#19423

Depositor

Ronald Conaway, Joan Conaway

Article

Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

Insert

adhesion regulating molecule 1 (ADRM1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pET28a-Vx3(A7)

Plasmid

#35526

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

Vx3(A7)

Use

Tags

His6

Expression

Bacterial

Mutation

Changed G2 to P

Promoter

T7

Available since

April 6, 2012

Availability

Academic Institutions and Nonprofits only

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pET12a-AL-103 FL

Plasmid

#47090

Depositor

Marina Ramirez-Alvarado

Article

Martin et al Amyloid. 2010 Sep;17(3-4):129-36. doi: 1

Insert

AL-103 (IGKV3-20 Human)

Use

Tags

ompT

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET30a-YedK

Plasmid

#86884

Purpose

Bacterial expression of E. coli SRAP ortholog YedK

Depositor

Douglas Feldman

Article

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

YedK (yedK Eschericia coli)

Use

Tags

6xHis, HA tag, and S-Tag

Expression

Bacterial

Mutation

Promoter

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b-12x

Plasmid

#61699

Purpose

Expresses PTDH mutant Opt12x

Depositor

Huimin Zhao

Article

Johannes et al Biotechnol Bioeng. 2007 Jan 1;96(1):18-2

Insert

Opt12x

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 3, 2015

Availability

Academic Institutions and Nonprofits only
pET28a-MSP1D1deltaH4/2

Plasmid

#71712

Purpose

Membrane scaffold protein (MSP), half of helix 4 truncated

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Use

Tags

6xHis and TEV site

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-soNadV

Plasmid

#83363

Purpose

Expresses nadV from Shewanella oneidensis in Escherichia coli

Depositor

George Cătălin Marinescu

Article

Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

Insert

nadV

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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