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Plasmid#213717PurposeFor lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIBY55 driven by an hPGK promoter.DepositorInsertEGFP
UseTagsGSTExpressionMammalianMutationPromoterhPGKAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-GST-EGFP-GPICEAM7
Plasmid#213718PurposeFor lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 driven by an hPGK promoter.DepositorInsertEGFP
UseTagsGSTExpressionMammalianMutationPromoterhPGKAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GST-EGFP-GPICEAM7
Plasmid#213723PurposeFor overexpression of a gene, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 driven by an SV40 promoter.DepositorInsertEGFP
UseTagsV5-tag-GSTExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TST-EGFP-GPIDAF
Plasmid#213712PurposeFor overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an SV40 promoter.DepositorInsertEGFP
UseTagsTwin-strep-tag and V5-tagExpressionMammalianMutationPromoterSV40Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TST-EGFP-GPIBY55
Plasmid#213711PurposeFor overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an SV40 promoter.DepositorInsertEGFP
UseTagsTwin-strep-tag and V5-tagExpressionMammalianMutationPromoterSV40Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGIGYF-Y58AY60AM65A_AH
Plasmid#148907PurposeInsect Expression of DmGIGYFDepositorInsertDmGIGYF (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2-W390A_P
Plasmid#147236PurposeInsect Expression of CeAIN2-W390ADepositorInsertCeAIN2-W390A
UseTagsExpressionInsectMutationDeletion of two AA SD 378-379 compared to the seq…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2-3xWmut_P
Plasmid#147231PurposeInsect Expression of CeAIN2-3xWmutDepositorInsertCeAIN2-3xWmut
UseTagsExpressionInsectMutationDeletion of two AA SD 378-379 compared to the seq…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2-W282AW366A_P
Plasmid#147233PurposeInsect Expression of CeAIN2-W282AW366ADepositorInsertCeAIN2-W282AW366A
UseTagsExpressionInsectMutationDeletion of two AA SD 378-379 compared to the seq…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2-W282AW390A_P
Plasmid#147234PurposeInsect Expression of CeAIN2-W282AW390ADepositorInsertCeAIN2-W282AW390A
UseTagsExpressionInsectMutationDeletion of two AA SD 378-379 compared to the seq…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2-W282A_O
Plasmid#147128PurposeInsect Expression of CeAIN2-W282ADepositorInsertCeAIN2-W282A
UseTagsExpressionInsectMutationDeletion of two AA SD 378-379 compared to the seq…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2-W366A_O
Plasmid#147129PurposeInsect Expression of CeAIN2-W366ADepositorInsertCeAIN2-W366A
UseTagsExpressionInsectMutationDeletion of two AA SD 378-379 compared to the seq…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-delM2_H
Plasmid#146521PurposeInsect Expression of DmGW182-delM2DepositorInsertDmGW182-delM2 (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-12xmut_F
Plasmid#146320PurposeInsect Expression of DmGW182-12xmutDepositorInsertDmGW182-12xmut (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT GFP CL1 DAmP
Plasmid#182513PurposeC-SWAT compatible vector for C-terminal tagging with GFP CL1 followed by the DAmP cassette. The stop codon after the CL1 degron is immediately followed by the Hygromycin B (Hph) resistance cassette.DepositorInsertCL1 and DAmP
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-delMBR-pEGFP-C1
Plasmid#177432PurposeTo express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) deleted (Bik(1-136)). Compare to full length Addgene #166737.DepositorInsertBik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
UseTagsVenusExpressionMammalianMutationBik MBR removed (after position 136)PromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2-W366AW390A_P
Plasmid#147235PurposeInsect Expression of CeAIN2-W366AW390ADepositorInsertCeAIN2-W366AW390A
UseTagsExpressionInsectMutationDeletion of two AA SD 378-379 compared to the seq…PromoterAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat_1-225_A
Plasmid#145870PurposeInsect Expression of DmHPat_1-225DepositorInsertDmHPat_1-225 (Patr-1 Fly)
UseTagsExpressionInsectMutationone non silent mutation N218K compared to the seq…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat_500-968_A
Plasmid#145873PurposeInsect Expression of DmHPat_500-968DepositorInsertDmHPat_500-968 (Patr-1 Fly)
UseTagsExpressionInsectMutationone silent mutation A1758G compared to the sequen…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsXRN1_1-1173_K
Plasmid#146785PurposeMammalian Expression of HsXRN1_1-1173DepositorInsertHsXRN1_1-1173 (XRN1 Human)
UseTagsExpressionMammalianMutationone silent mutation compared to the sequence give…PromoterAvailable sinceNov. 11, 2020AvailabilityAcademic Institutions and Nonprofits only