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PvLEA4_repeats_k3_26_mCh-2xFKBP (pBS1118)

Plasmid

#185313

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_26 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k3_26

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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DHN_VrDHN1a_mCh-2xFKBP (pBS1117)

Plasmid

#185312

Purpose

For the mammalian expression of the riverbank grape plant protein DHN_VrDHN1a attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DHN_VrDHN1a

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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DSUP_RAMVA_1-208_mCh-2xFKBP (pBS1116)

Plasmid

#185311

Purpose

For the mammalian expression of the tardigrade protein DSUP_RAMVA_1-208 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DSUP_RAMVA_1-208

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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DHR93_Nccapped_mCh-2xFKBP (pBS1115)

Plasmid

#185310

Purpose

For the mammalian expression of the synthetic protein DHR93_Nccapped attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DHR93_Nccapped

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE3_D125I_47-300_mCh-2xFKBP (pBS1114)

Plasmid

#185309

Purpose

For the mammalian expression of the human protein ApoE3_D125I_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE3_D125I_47-300

Use

Tags

Expression

Mammalian

Mutation

D125I

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE3_47-300_mCh-2xFKBP (pBS1113)

Plasmid

#185308

Purpose

For the mammalian expression of the human protein ApoE3_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE3_47-300

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE4_47-300_mCh-2xFKBP (pBS1112)

Plasmid

#185307

Purpose

For the mammalian expression of the human protein ApoE4_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE4_47-300

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE2_47-300_mCh-2xFKBP (pBS1111)

Plasmid

#185306

Purpose

For the mammalian expression of the human protein ApoE2_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE2_47-300

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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CAHS3hypdu-mCh-2xFKBP (pBS1110)

Plasmid

#185305

Purpose

For the mammalian expression of the tardigrade protein CAHS3hypdu attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS3hypdu

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pRD198

Plasmid

#163101

Purpose

Expression of mEGFP_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

mEGFP_H-NSdbd

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-APP-mGFP

Plasmid

#196704

Purpose

Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells

Depositor

Martino Calamai

Article

Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

Insert

Amyloid Precursor Protein 695 isoform (APP Human)

Use

Tags

mCherry and mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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mBFP-APP-mGFP

Plasmid

#196694

Purpose

Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells

Depositor

Martino Calamai

Article

Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

Insert

Amyloid Precursor Protein 695 isoform (APP Human)

Use

Tags

mEGFP and mtagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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DNA-CARzeta-GFP

Plasmid

#89344

Purpose

2nd generation lentiviral vector which expresses DNA-CAR receptor fused to GFP

Depositor

Ron Vale

Article

Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

Insert

DNA-CARzeta (CD247, CD86 Human)

Use

Lentiviral

Tags

SNAPf tag and mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pBactin-Akna-mOrange2

Plasmid

#196865

Purpose

Expression of the centrosomal protein Akna fused to mOrange2

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Akna-mOrange2 (Akna Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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KillerRed-LacI-NLS

Plasmid

#215102

Purpose

Expresses KillerRed-LacI-NLS in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

KillerRed-LacI-NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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FokI-mCherry-LacI-NLS

Plasmid

#215096

Purpose

Expresses FokI-mCherry-LacI-NLS in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

FokI-mCherry-LacI-NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-His6-FnCas9GFP

Plasmid

#130966

Purpose

expression of FnCas9-GFP in bacterial cells

Depositor

Debojyoti Chakraborty

Article

Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

Insert

HA-NLS-FnCas9

Use

CRISPR

Tags

6xHis-mEGFP-TEV-HA-NLS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-APPP1-mGFP

Plasmid

#196705

Purpose

As mCherry-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activity

Depositor

Martino Calamai

Article

Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

Insert

Amyloid Precursor Protein 695 isoform (APP Human)

Use

Tags

mCherry and mEGFP

Expression

Mammalian

Mutation

Lys to Val substitution at position 612

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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mBFP-APPP1-mGFP

Plasmid

#196695

Purpose

As mBFP-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activity

Depositor

Martino Calamai

Article

Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

Insert

Amyloid Precursor Protein 695 isoform (APP Human)

Use

Tags

mEGFP and mtagBFP2

Expression

Mammalian

Mutation

Lys to Val substitution at position 612

Promoter

CMV

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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FokI(inactive)-mCherry-LacI-NLS

Plasmid

#215100

Purpose

Expresses FokI(inactive)-mCherry-LacI-NLS in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

FokI(inactive)-mCherry-LacI-NLS

Use

Tags

Expression

Mammalian

Mutation

FokI(inactive) mutant D69A.

Promoter

CMV

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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