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pLenti_CMV:Flag-OeNL

Plasmid

#208850

Purpose

Expresses FLAG epitope-tagged OeNL "Orange" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

OeNL Nanolantern

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1_Zeo_TetO_FLAG-AT1R

Plasmid

#222856

Purpose

Mammalian expression plasmid for FLAG-tagged AT1R

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT1R (AGTR1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pTFG027_Spike_sACE2-resistant_D614G_F486S_3xFlag

Plasmid

#191572

Purpose

Mutated SARS-CoV-2 Spike under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. Encodes F486S mutation. For expression and pseudotyping lentiviral particles.

Depositor

Joshua Leonard

Article

Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

Insert

SARS-CoV-2 Spike Protein (S Synthetic)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

D614G and F486S (confers reduced binding affinity…

Promoter

CMV

Available since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1_BN006A-P_V5_FLAG_C

Plasmid

#218062

Purpose

RESOLUTE SLC binder lentiviral plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN006A-P

Use

Lentiviral

Tags

V5-FLAG

Expression

Mutation

Promoter

Available since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1_BN001M-T_V5_FLAG_C

Plasmid

#218063

Purpose

RESOLUTE SLC binder lentiviral plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN001M-T

Use

Lentiviral

Tags

V5-FLAG

Expression

Mutation

Promoter

Available since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1_BN006A-P_V5_mNG_FLAG_C

Plasmid

#218076

Purpose

RESOLUTE SLC binder lentiviral plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN006A-P

Use

Lentiviral

Tags

FLAG-mNeonGreen-V5

Expression

Mutation

Promoter

Available since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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pIRESneo-FLAG/HA-FL

Plasmid

#215839

Purpose

Expression of FLAG/HA tagged firefly luciferase (FL)

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

FLAG/HA tagged firefly luciferase (FL)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG_ALX4-Flag-HA

Plasmid

#215028

Purpose

Mammalian expression of human ALX4

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

ALX homeobox 4 (ALX4 Human)

Use

Tags

Flag-HA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a_DIO_HA/FLAG_muMASS_LF

Plasmid

#213394

Purpose

Loss of function variant of uMASS_1 opioid sensor. AAV virus production for Cre dependent expression.

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_LF

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a_DIO_HA/FLAG_muMASS

Plasmid

#213393

Purpose

uMASS_1 opioid sensor. AAV Virus production for Cre dependent expression

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_1

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_HA_Flag-uMASS_LF

Plasmid

#209761

Purpose

AAV virus production for neuonal expression of uMASS (loss-of-function) under hSyn promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_LF

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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MIGR1_FLAG_TAL1-short_GFP

Plasmid

#210637

Purpose

Overexpression of TAL1-short

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1-short (TAL1 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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MIGR1_FLAG_TAL1-long_GFP

Plasmid

#210638

Purpose

Overexpression of TAL1-long

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1-short (TAL1 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV7.1_3xFLAG-TAOK1

Plasmid

#172991

Purpose

constitutive expression of FLAG-tagged TAOK1

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-TAOK1 (TAOK1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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3xFlag-CEP83

Plasmid

#200442

Purpose

Expresses 3xFlag-tagged mouse CEP83 in mammalian cells

Depositor

Ken-Ichi Takemaru

Insert

CEP83 (CCDC41 Human)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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TXNIP-py-FLAG

Plasmid

#200092

Purpose

TXNIP py motif mutant with C-terminal 3xFLAG tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

TXNIP (TXNIP Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

py motifs mutated (aa331-334 and 375-378)

Promoter

CMV

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT 6FLAG

Plasmid

#182509

Purpose

C-SWAT compatible vector for C-terminal tagging with 6 FLAG

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

6 FLAG

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG_BIRC6_delta(Helix)

Plasmid

#197969

Purpose

Mammalian cell expression of 3xFLAG-BIRC6_delta(Helix)

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

L1332V, delta(1616-1666)

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_F3194S

Plasmid

#187140

Purpose

mammalian cell expression of FLAG-HUWE1 F3194S

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

1xFLAG

Expression

Mammalian

Mutation

F3194S

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG-HUWE1_H669Q

Plasmid

#187138

Purpose

mammalian cell expression of 3xFLAG-HUWE1 H669Q

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

H669Q

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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