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Plasmid#127735PurposeYeast pathway position 5. PYC transcription unit with the PGK1 promoter and TPI1 terminator.DepositorInsertpyruvate carboxylase (PYC1 Synthetic, Budding Yeast)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPpgk1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-de-Ppxr1-PYC-Tnat1
Plasmid#127736PurposeYeast pathway position 5. PYC transcription unit with the PXR1 promoter and NAT1 terminator.DepositorInsertpyruvate carboxylase (PYC1 Synthetic, Budding Yeast)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPpxr1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-ef-Psbtdh3-CSC-Tefm1
Plasmid#127742PurposeYeast pathway position 6. CSC transcription unit with the S. bayanus TDH3 promoter and EFM1 terminator.DepositorInsertcitrate synthase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPsbtdh3Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-f3-Peno2-ACDH-Ttal1
Plasmid#127743PurposeYeast pathway position 7. ACDH transcription unit with the ENO2 promoter and TAL1 terminator.DepositorInsertacetylating acetaldehyde dehydrogenase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPeno2Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-f3-Pvma6-ACDH-Trps14a
Plasmid#127744PurposeYeast pathway position 7. ACDH transcription unit with the VMA6 promoter and RPS14A terminator.DepositorInsertacetylating acetaldehyde dehydrogenase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPvma6Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-f3-Prpl28-ACDH-Tecm10
Plasmid#127745PurposeYeast pathway position 7. ACDH transcription unit with the RPL28 promoter and ECM10 terminator.DepositorInsertacetylating acetaldehyde dehydrogenase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPrpl28Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-f3-Ptdh3-ACDH-Trpl3
Plasmid#127746PurposeYeast pathway position 7. ACDH transcription unit with the TDH3 promoter and RPL3 terminator.DepositorInsertacetylating acetaldehyde dehydrogenase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPtdh3Available sinceAvailabilityAcademic Institutions and Nonprofits only -
eeBxb1_IVT
Plasmid#222342PurposePlasmid for evolved and engineered Bxb1 mRNA in vitro transcription templateDepositorInsertCodon-optimized evolved and engineered Bxb1
UseTemplate for in vitro transcriptionTagsExpressionMutationPromoterT7 (inactivated)Available sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 pegRNA1 with trimmed attP
Plasmid#222346PurposepegRNA 1 plasmid used for PASSIGE-mediated Bxb1 attP installationDepositorInsertAAVS1 attP-installation pegRNA1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 pegRNA2 with trimmed attP
Plasmid#222347PurposepegRNA 2 plasmid used for PASSIGE-mediated Bxb1 attP installationDepositorInsertAAVS1 attP-installation pegRNA2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-Nme2ABE8e
Plasmid#189928PurposePlasmid encoding Nme2ABE8e driven by the CMV promoter for plasmid transfection.DepositorInsertNme2ABE8e
UseTagsNLSExpressionMammalianMutationNme2Cas9 D16APromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-CjABE8e
Plasmid#189929PurposePlasmid encoding CjABE8e driven by the CMV promoter for plasmid transfection.DepositorInsertCjABE8e
UseTagsExpressionMammalianMutationCjCas9 D8APromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-SauriABE8e
Plasmid#189927PurposePlasmid encoding SauriABE8e driven by the CMV promoter for plasmid transfection.DepositorInsertSauriABE8e
UseTagsExpressionMammalianMutationNme2Cas9 D16APromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6409
Plasmid#47048PurposeBglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coliDepositorInsertCodon-optimized sequences for MEV pathway expression in E. coli to produce limonene
UseSynthetic Biology; BglbrickTagsExpressionBacterialMutationPromoterPlacUV5Available sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
amajLime chromoprotein
Plasmid#117843PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses amajLime chromoprotein in E. coliDepositorInsertpromoter, RBS, amajLime
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
aeBlue chromoprotein
Plasmid#117846PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses aeBlue chromoprotein in E. coliDepositorInsertpromoter, RBS, aeBlue
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS1ClacZ
Plasmid#55171PurposelacZ-reporter vector, integration at amyE, ampr, cmrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoternoneAvailable sinceAug. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
asPink chromoprotein
Plasmid#117838PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses asPink chromoprotein in E. coliDepositorInsertpromoter, RBS, asPink
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS2E
Plasmid#55169PurposeEmpty vector, integration at lacA, ampr, mlsrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoternoneAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
fwYellow chromoprotein
Plasmid#117841PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow chromoprotein in E. coliDepositorInsertpromoter, RBS, fwYellow
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only