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p3E-EHD2a
Plasmid#109568PurposeMultisite gateway vector for 3' tagging with EHD2aDepositorInsertEHD2a
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
JK305: pMVP/Blast-DEST
Plasmid#121855PurposepMVP destination vector, empty expression vector backbone w/ Blast selection cassetteDepositorTypeEmpty backboneUsePmvp gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
JK104: pMVP-DEST
Plasmid#121851PurposepMVP destination vector, empty expression plasmid backboneDepositorTypeEmpty backboneUsePmvp gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI177
Plasmid#126077PurposeModule 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP1-10 and expression in plant cellDepositorInsertsccdB Cassette (BsaI)
GFP1-10
UseBinary, module 1Tagslinker (GTGGGGS)ExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI129
Plasmid#126210PurposeModule 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x7 and expression in plant cellDepositorInsertsccdB Cassette (BsaI)
GFP11x7 (5 aa linker)
UseBinary, module 1TagsExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI161
Plasmid#126211PurposeModule 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11 and expression in plant cellDepositorInsertsccdB Cassette (BsaI)
GFP11
UseBinary, module 1TagsExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
BPK2013-BsCas12b
Plasmid#121952PurposeE. coli expression, protein purificationDepositorInsertBsCas12b
UseTags10xHis tag and 3xHA tagExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC17
Plasmid#118875Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal tagRFPDepositorInsertNcoI-HIS6-3C--NotI-ccdB-AscI-tagRFP-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease and tagRFPExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC16
Plasmid#118870Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal EGFPDepositorInsertNcoI-HIS6-3C--NotI-ccdB-AscI-EGFP-stop-HindIII cassette
UseTagsEGFP and HIS6, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC218
Plasmid#118907Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS10, cleavable with 3C protease and N-terminal YBBR tagDepositorInsertNcoI-HIS10-3C-YBBR-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS10, cleavable with 3C protease, YBBR tagExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC18
Plasmid#118868Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal tagRFPDepositorInsertNcoI-HIS6-3C-tagRFP-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease, tagRFPExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC202
Plasmid#118880Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mKate2, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-mKate2-3C-HIS6-stop-HindIII cassette
UseTagsmKate2, HIS6, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC139
Plasmid#118872Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFPDepositorInsertNcoI-HIS6-3C--NotI-ccdB-AscI-mGFP-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease and mGFPExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEG R1-ChlorR-R3 #BGV148
Plasmid#48942PurposeContains the Gateway selection cassette (ccdB ChlorR) flanked by AttR1 and AttR3 sites for the creation of Destination vectorsDepositorTypeEmpty backboneUseGateway cloning vectorTagsExpressionMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
actc1b- mKate2-KDEL-pA
Plasmid#109494PurposeMuscle specific zebrafish expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequenceDepositorInsertactc1b- mKate2-KDEL-pA
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-8
Plasmid#163136PurposepLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterP(rhaB)Available sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-EXOB-11
Plasmid#197688PurposePhytobrick (MoClo) Level 0 PartDepositorInsertEXOB enhancer-blocking insulator
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Zebrabow-GateDest
Plasmid#118221PurposeFor cloning promoter into a Zebrabow vector with Gateway reaction. Only compatible with single site Gateway. Not compatible with Tol2-kit.DepositorInsertBrainbow 1.0 L - ccdB survival cassette
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-Bin1b
Plasmid#109509PurposeMuscle specific zebrafish expression of mKate2 fused to Bin1bDepositorInsertBin1b (bin1b Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only
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