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pcDNA3.2/DEST/hTfR-HA

Plasmid

#69610

Purpose

human transferrin receptor with an HA tag fused at the C-terminus

Depositor

Robin Shaw

Article

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

human transferrin receptor (TFRC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti Dest BlastR R1-R2

Plasmid

#84574

Purpose

Gateway destination vector expressing Blasticidin resistance under a PGK promoter

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Type

Empty backbone

Use

Lentiviral; Gateway

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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AktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax

Plasmid

#173862

Purpose

Mammalian expression of Akt PH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax (PIP3 marker and opto-PI3K)

Depositor

Takao Nakata

Article

Kakumoto et al PLoS One. 2013 Aug 7;8(8):e70861. doi: 1

Insert

AktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax

Use

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-H-Ras_V12

Plasmid

#39504

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 (HRAS Human)

Use

Tags

Expression

Mammalian

Mutation

G12V

Promoter

CMV

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti Dest PuroR R1-R2

Plasmid

#84575

Purpose

Gateway destination vector expressing Puromycin resistance under a PGK promoter

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Type

Empty backbone

Use

Lentiviral; Gateway

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLL5.0 E-cadherin shRNA/mE-cadherin-mCherry

Plasmid

#101282

Purpose

Lentiviral expression of shRNA and mouse E-cadherin -mCherry

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

E-cadherin

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_WT-PolyA

Plasmid

#112284

Purpose

Fluorescently labels WT human YAP1-2gamma isoform (504 aa)

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT human YAP1-2gamma

Promoter

EF-1 alpha

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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LVDP-CArG-RE-GPR

Plasmid

#89762

Purpose

Quantitative measurement of CArG response element activity.

Depositor

Stelios Andreadis

Article

Mistriotis et al Stem Cells. 2017 Jan;35(1):207-221. doi:

Insert

6X CArG-RE

Use

Lentiviral

Tags

CMV-ZsGreen

Expression

Mammalian

Mutation

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-Dynamin 2 K44A

Plasmid

#22301

Depositor

Pietro De Camilli

Article

Ochoa et al J Cell Biol. 2000 Jul 24. 150(2):377-89.

Insert

Dynamin 2 K44A (Dnm2 Rat)

Use

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

K44A

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-HA-ARHGAP29

Plasmid

#104154

Purpose

mammalian expression of HA tagged ARHGAP29

Depositor

Marius Sudol

Article

Qiao et al Cell Rep. 2017 May 23;19(8):1495-1502. d

Insert

ARHGAP29 (ARHGAP29 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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Lifeact-7-iRFP670

Plasmid

#103032

Purpose

Expression of iRFP670-tagged Lifeact with G418 selection in mammalian cells

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

Lifeact

Use

Tags

iRFP670

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-ACTB_sgRNA

Plasmid

#183885

Purpose

pX459V2.0-HypaCas9 plasmid with ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ACTB sgRNA spacer (ACTB Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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His-MBP_G4G6 in pRSF

Plasmid

#188454

Purpose

Express in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags.

Depositor

Roberto Dominguez

Article

Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

Insert

G4G6 (GSN Human)

Use

Tags

6xHis-MBP

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLXSN-H-Ras_N17

Plasmid

#39537

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS N17 (HRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

N17

Promoter

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pB80-KIF1A(1-383)-GFP-SSPB(milli)

Plasmid

#174626

Purpose

Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(milli)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-383)-GFP-SSPB(milli) (Kif1a Mouse, Synthetic)

Use

Tags

GFP-SSPB(milli)

Expression

Mammalian

Mutation

mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del; SSPB:…

Promoter

Chicken beta-actin

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET28b_RanT24N

Plasmid

#186271

Purpose

Expression vector of His-RanT24N in E.coli

Depositor

Masahito Ikawa

Article

Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

GTP-binding nuclear protein Ran (Ran Mouse)

Use

Tags

His tagged and His-tagged at N terminal

Expression

Bacterial

Mutation

Changed T24 into N

Promoter

T7 promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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EHD2-T72A-mEGFP

Plasmid

#45934

Purpose

dominant-negative EHD2 mutant

Depositor

Ari Helenius

Article

Stoeber et al EMBO J. 2012 May 16;31(10):2350-64. doi:

Insert

Eps-15 homology domain-containing protein2 (EHD2 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

T 72 changed to A (T72A)

Promoter

CMV

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pB80-hsKIF5B(1-560)-L-GFP

Plasmid

#193716

Purpose

Expression of GFP tagged human kinesin-1 (KIF5B aa1-560)

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-560)-L-GFP (KIF5B Human, Synthetic)

Use

Tags

EGFP

Expression

Mammalian

Mutation

hsKIF5B(aa1-560);

Promoter

Chicken beta-actin

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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phage - ubc - nls - 2xmcp - egfp - BirA*

Plasmid

#131132

Purpose

BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

Depositor

Ralf Jansen

Article

Mukherjee et al Proc Natl Acad Sci U S A. 2019 Jun 25;11

Insert

NLS-HA-2xMCP-EGFP-BirA*

Use

Lentiviral

Tags

NLS, HA

Expression

Mammalian

Mutation

BirA* = BIrA >R118G

Promoter

human ubiquitin C (UBC)

Available since

Aug. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-EPLIN alpha delta LIM

Plasmid

#40933

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN alpha (LIMA1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

LIM domain deleted- amino acids 221-295

Promoter

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only