Showing: 861 - 880 of 1248 results
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Plasmid#149390PurposeP. aeruginosa PA14 CRISPR2 locus, with 5bp addition downstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutation5bp inserted downstream of the IHFprox sitePromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_+10D
Plasmid#149391PurposeP. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutation10bp inserted downstream of the IHFprox sitePromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_IHF_Opt
Plasmid#149395PurposeP. aeruginosa PA14 CRISPR2 locus, with "optimized" IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutationIHFprox site is optimized at key positions needed…PromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_motif_scram
Plasmid#149397PurposeP. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motifDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutationUpstream motif recognized by Cas1-2/3 during spac…PromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
pTA-Mob 2.0
Plasmid#149662PurposeConjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.DepositorTypeEmpty backboneUseSynthetic Biology; Conjugation helper plasmidTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
ArpC3-FLAG_pLib
Plasmid#173680PurposeArpC3 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expressionDepositorInsertArpC3
UseTagsTEV cleavage site and FLAG tagExpressionInsectMutationPromoterpolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_IRdist_scram
Plasmid#162319PurposeP. aeruginosa PA14 CRISPR2 locus, with a scrambled distal Inverted Repeat of the upstream motifDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutationThe distal Upstream Motif site is scrambledPromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
pBS1479-HTP-KanMX6
Plasmid#154085PurposeFor C-terminal his6-TEV-ProtA-tagging in S. pombe using KanMX6 as resistance marker.DepositorInserthis6-TEV-protein A-KanR
UsePcr-based tagging in yeastTagshis6-TEV-protein AExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFastBac-His-MBP-SCov2-nsp12
Plasmid#154759PurposeExpression of SARS-CoV-2 nsp12DepositorInsertSARS-CoV-2 nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionInsectMutationPromoterPolyhedronAvailabilityAcademic Institutions and Nonprofits only -
pSBL_DK186
Plasmid#156422PurposeMammalian expression of 3a-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cellsDepositorInsertSARS-CoV-2 3a protein
UseTagsEGFP-HisExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSBL_DK193
Plasmid#156424PurposeInsect cell expression of 3a(Q57H)-sfGFP, needs to be introduced into baculoviral bacmidDepositorInsertSARS-CoV-2 3a protein (Q57H)
UseTagssfGFP-HisExpressionInsectMutationQ57HPromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pSBL_DK198
Plasmid#156425PurposeInsect cell expression of 3a delta N(aa 42-275)-sfGFP, needs to be introduced into baculoviral bacmidDepositorInsertSARS-CoV-2 3a protein (aa 42-275)
UseTagssfGFP-HisExpressionInsectMutationaa 42-275PromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-Q22YU3-2xStrep
Plasmid#170315PurposePlasmid for insect cell expression of Tetrahymena thermophila Q22YU3 C-terminally tagged with 2x Strep-tag IIDepositorInsertQ22YU3 (TTHERM_00122270 Tetrahymena thermophila)
UseTagsStrep-Tag IIExpressionInsectMutationPromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-Q22YU3 del C3-2xStrep
Plasmid#170316PurposePlasmid for insect cell expression of Tetrahymena thermophila Q22YU3 with a C-terminal truncation (C3-finger). Tagged with 2x Strep-tag II at the C-terminus.DepositorInsertQ22YU3 C-terminal truncation (Delta-C3)
UseTagsStrep-Tag IIExpressionInsectMutationDeletion of C-terminal C3-fingerPromoterpolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual α1B/β2B Tubulin
Plasmid#170553PurposeEncodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.DepositorInsertsTUBA1B
TUBB2B
UseTagsAP linker, GS linker (GGSGG), His10 tag, L21 enha…ExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDONR207-MUM2sp-RglA
Plasmid#170720PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglA (AN7135.2)DepositorInsertRhamnogalacturonan lyase A
UseGateway donor vector / entry cloneTagsExpressionMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207-MUM2sp-RglB
Plasmid#170721PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglB (AN6395.2)DepositorInsertRhamnogalacturonan lyase B
UseGateway donor vector / entry cloneTagsExpressionMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207-MUM2sp-PelA
Plasmid#170722PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme PelA (AN2331.2)DepositorInsertPectin lyase A
UseGateway donor vector / entry cloneTagsExpressionMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0
Plasmid#174081PurposeExpression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.DepositorInsertNES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
UseTagsNES-FlagExpressionMammalianMutationL270G, Y271L, N311G, C313APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLIB-HIS-TEV-scCAK1
Plasmid#177014PurposeGenerate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEVDepositorInsertCAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)
UseTagsPolyhistidine-TEVExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 861 - 880 of 1248 results