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Plasmid#120572PurposeExpresses Flag-HA-6xHIS-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus productionDepositorInsertNRAS (NRAS Human)
UseLentiviralTagsFlag-HA-6xHISExpressionMammalianMutationQ61K, E37APromoterCMVAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-3UTR
Plasmid#202550PurposeReady for cloning and heterologous expression of genes in mammalian cells with protein export to cultivation mediaDepositorTypeEmpty backboneUseTagsATG initiation codon followed by an IGKV3 signal …ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) K-Ras(WT)
Plasmid#224283PurposeCell transfection and expression of K-RasDepositorInsertK-Ras (KRAS Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) K-Ras(G12C)
Plasmid#224284PurposeCell transfection and expression of K-Ras (G12C)DepositorInsertK-Ras (KRAS Human)
UseTagsExpressionMammalianMutationG12CPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVET-HA-K-RasG12V-IRES-GFP
Plasmid#107140PurposeA bicistronic lentiviral mammalian expression vector for HA-KRasG12V(4B) and GFPDepositorInsertHA-tagged Kras4B(G12V) and GFP (KRAS Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhEF-1alphaAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
4LG6
Plasmid#60093PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertANKRA:JMC023-D07:C211743 (ANKRA2 Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXL001
Plasmid#26122DepositorInserttTR-KRAB
UseLentiviralTagsIRES-PuroExpressionMutationPromoterAvailable sinceJune 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMP315
Plasmid#134994PurposeKRAB-m6A Reader. Repress transcription near Gm6ATC sites. pUBC-KRAB-NLS-DpnI(aa146-254)DepositorInsertpUBC-KRAB-NLS-DpnI(aa146-254)
UseTagsDpnI(aa146-254) and KRAB domainExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
AA093
Plasmid#216042PurposeFragmid fragment: (C' terminus) ZIM3 KRABDepositorHas ServiceCloning Grade DNAInsertNpNLS_v1.1; KRAB_v3.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA092
Plasmid#216041PurposeFragmid fragment: (C' terminus) ZIM3 KRABDepositorHas ServiceCloning Grade DNAInsertNpNLS_v1.1; KRAB_v2.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA095
Plasmid#216043PurposeFragmid fragment: (C' terminus) ZNF10 KRABDepositorHas ServiceCloning Grade DNAInsertNpNLS_v1.1; KRAB_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-Xlrbpa
Plasmid#171547PurposeEncoding part of the Xenopus Xlrbpa-2 protein from M120 to L202DepositorInsertXlrbpa (prkra.L Frog)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
p101_CRISPRai_PiggyBac
Plasmid#213777PurposeExpresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.DepositorInsertsVPR-dSaCas9
dSpCas9-KRAB-BFP
zeocin
Tet-On transactivator
UseCRISPRTagsExpressionMutationPromoterEF1a (EF-1-alpha intron a) and Tet_On_TREAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA091
Plasmid#215979PurposeFragmid fragment: (N' terminus) ZNF10 KRABDepositorHas ServiceCloning Grade DNAInsertStart; BPNLS_v1.1; KRAB_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA088
Plasmid#215978PurposeFragmid fragment: (N' terminus) ZIM3 KRABDepositorHas ServiceCloning Grade DNAInsertStart; BPNLS_v1.1; KRAB_v2.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-Xlrbpa-dsRBD(+6)
Plasmid#171548PurposeEncoding part of the Xenopus Xlrbpa-2 protein from M120 to L202DepositorInsertXlrbpa (prkra.L Frog)
UseTagsExpressionBacterialMutationa six amino acid_TRLTEG_insertion in the L1 regionPromoterAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMTL5
Plasmid#127967PurposeDHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locusDepositorInsertAAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
UseTALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTL3
Plasmid#127966Purposeconstitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locusDepositorInsertAAVS1-CAG-KRAB-dCas9-BFP-KRAB
UseTALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-tdTomato
Plasmid#128434PurposeExpresses tdTomato under control of eukaryotic Ef1a promoter after Flp-dependent recombinationDepositorHas ServiceAAV1InserttdTomato
UseAAVTagsExpressionMammalianMutationPromoterEf1aAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-ChrimsonR-tdTomato
Plasmid#171027PurposeExpresses ChrimsonR-tdTomato fusion after Flp-dependent recombinationDepositorHas ServiceAAV1 and AAV9InsertChrimsonR-tdTomato
UseAAVTagsChrimsonR is fused to tdTomatoExpressionMutationPromoterEf1aAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only