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Addgene
Showing: 861 - 880 of 1875 results

  1. p3805

    Plasmid
    #198147
    Purpose
    Mammalian expression of LAMP1-miniSOG
    Depositor
    Michael Hensel
    Insert
    PCMV::LAMP1::miniSOG::mCherry (LAMP1 Human)
    Use
    Tags
    miniSOG-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    198147-plasmid-map-sequence-id-388090

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  2. p3806

    Plasmid
    #198148
    Purpose
    Mammalian expression of LAMP1-miniSOG
    Depositor
    Michael Hensel
    Insert
    PCMV::LAMP1::tdminiSOG::mCherry (LAMP1 Human)
    Use
    Tags
    tdminiSOG-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    198148-plasmid-map-sequence-id-388049

    Enlarge

  3. MUT_S1-H1,NR

    Plasmid
    #218395
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, both randomly mutated to avoid sequence repeats, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Availability
    Academic Institutions and Nonprofits only
    218395-plasmid-map-sequence-id-433911

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  4. MUT_S1-spacer-H1

    Plasmid
    #218399
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron following a neutral spacer, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Availability
    Academic Institutions and Nonprofits only
    218399-plasmid-map-sequence-id-436404

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  5. MINA

    Plasmid
    #39054
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MINA (RIOX2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  8. pCAG-Scarlet-Kif5a

    Plasmid
    #118745
    Purpose
    Expresses scarlet tagged Kif5a
    Depositor
    Beverly Koller
    Insert
    Kif5a cDNA (Kif5a Mouse)
    Use
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    118745-plasmid-map-sequence-id-229089

    Enlarge

  10. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-ZsG

    Plasmid
    #192665
    Purpose
    3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1
    Depositor
    Jeffrey Miner
    Insert
    dCas9-VP64, MS2-VP64, ZsGreen1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    192665-plasmid-map-sequence-id-379871

    Enlarge

  11. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-Blast

    Plasmid
    #192666
    Purpose
    3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A Blast resistance marker
    Depositor
    Jeffrey Miner
    Insert
    dCas9-VP64, MS2-VP64
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    192666-plasmid-map-sequence-id-379913

    Enlarge

  12. lenti-dCas9-ZIM3-KRAB-BFP

    Plasmid
    #196712
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-KRAB(ZIM3)-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    196712-plasmid-map-sequence-id-388051

    Enlarge

  14. TDRD7 (3RCO)

    Plasmid
    #32051
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TDRD7 (TDRD7 Human)
    Use
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. pCAG-BE4max-P2A-EGFP (pRZ33)

    Plasmid
    #140083
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP
    Depositor
    Keith Joung
    Insert
    Human codon optimized pCAG BE4max with P2A-EGFP
    Use
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A (SpCas9)
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    140083-plasmid-map-sequence-id-292705

    Enlarge

  17. pHH0103 PDZK10 WW domain

    Plasmid
    #104285
    Purpose
    Bacterial expression of WW domain from PDZK10
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    PDZK10 WW domain (FRMPD4 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Availability
    Academic Institutions and Nonprofits only
    104285-plasmid-map-sequence-id-202571

    Enlarge

  18. pDONR221-appa

    Plasmid
    #192745
    Purpose
    Gateway entry vector encoding zebrafish appa
    Depositor
    Summer Thyme
    Insert
    appa (appa Zebrafish)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    S47N
    Promoter
    None
    Availability
    Academic Institutions and Nonprofits only
    192745-plasmid-map-sequence-id-377796

    Enlarge

  19. lenti-UCOE-dCas9-BFP-Zim3-KRAB

    Plasmid
    #196716
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-TagBFP-KRAB(ZIM3)
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    196716-plasmid-map-sequence-id-388040

    Enlarge

Showing: 861 - 880 of 1875 results