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Fuw-dCas9-Dnmt3a-P2A-tagBFP

Plasmid

#84569

Purpose

Lentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFP

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a (DNMT3A Human, Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mTagBFP2-P2A-EGFP

Plasmid

#184943

Purpose

Expresses blue fluorescent protein mTagBFP2 and EGFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

mTagBFP2-P2A-EGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ5465_pHR_hU6-crScaffold_EF1a-PuroR-T2A-BFP

Plasmid

#155307

Purpose

Rfx Cas13d guide cloning lentiviral vector with constitutively expressed puromycin resistance 2A-tagged with tagBFP

Depositor

Stanley Qi

Article

Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

Insert

Rfx Cas13d CRISPR RNA puromycin resistance 2A-tagged BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6, EF1a

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAF0689 Ef1a-Pa01_attB-BFP acceptor

Plasmid

#193472

Purpose

Acceptor plasmid for 3 plasmid recombination assay

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Pa01_attB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNASAM(gTetO)-TREGFP-PGKpuroBFP-W

Plasmid

#112927

Purpose

CRISPR-a reporter assay lentiviral vector (gTetO)

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6-gRNASAM(gTetO), TREGFP, PGKpuroGFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCA12-pHR-SFFV-BFP-B23

Plasmid

#199443

Purpose

Expression of BFP-B23 in mammalian cells

Depositor

Baohui Chen

Article

Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

Insert

B23 (NPM1 Human)

Use

Lentiviral

Tags

TagBFP

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-mTagBFP2-TEV-Strep-His

Plasmid

#210824

Purpose

Bacterial overexpression vector for C-terminally Strep and His tagged mTagBFP2 fluorescent protein

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

mTagBFP2

Use

Tags

TwinStrep, 10xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pPyCAG-tagBFP-3xNLS-IRES-Hph

Plasmid

#183608

Purpose

Mammalian expression vector for tagBFP-3xNLS from the CAG promoter. Confers hygromycin B resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

tagBFP-3xNLS

Use

Tags

3xNLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING-342STOP

Plasmid

#102588

Purpose

Lentivector to express STING delta 342 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

delta 342

Promoter

SFFV

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pME-loxP-mTagBFP2-stop-pA-loxP

Plasmid

#75158

Purpose

Floxed TagBFP2 (blue) fluorophore with stop codon for inducible lines.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

loxP-mTagBFP2-stop-pA-loxP

Use

Middle entry vector for multisite gateway three f…

Tags

Expression

Mutation

Promoter

n/a

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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LLP457 pGK-dCas9-Suntag-BFP

Plasmid

#100957

Purpose

BPF version of LLP339, Puro is replaced by BFP driven by EF1a, dCas9 with SungTagx10 driven by pGK, with 3xHA tag

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

dCas9, SunTag array (10xGCN4)

Use

CRISPR

Tags

3xHA and BFP

Expression

Mammalian

Mutation

D10A, H840A for dCas9

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV(gfp)U6sgRNA5(BbsI)-PGKpuroBFP

Plasmid

#102798

Purpose

Retrovirus for delivery of one sgRNA (self-targeting) - GFP-targeting control plasmid contains GFP instead of PuroR

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Inserts

sgRNA
EGFP

Use

CRISPR and Retroviral

Tags

Expression

Mammalian

Mutation

H231L

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pIRES-H3.3-G34R-FLAG-BFP

Plasmid

#206140

Purpose

Expression of a FLAG tagged human histone mutant H3.3-G34R with BFP

Depositor

Maria Castro

Insert

H3.3 (H3-3A Human)

Use

Tags

FLAG-eBFP

Expression

Mammalian

Mutation

G34R

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP

Plasmid

#154139

Purpose

Expresses the dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP fusion protein for targeted DNA methylation.

Depositor

Albert Jeltsch

Article

Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

Insert

dCas9, DNMT3a (catalytic domain), DNMT3l (C-terminal part), tagBFP

Use

Tags

3x FLAG and tagBFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-H3.3-G34R-FLAG-BFP

Plasmid

#209440

Purpose

Lentiviral packagable plasmid expressing FLAGGED human histone mutant H3.3-G34R with BFP

Depositor

Maria Castro

Insert

H3.3-G34R

Use

Tags

Expression

Mammalian

Mutation

G34R

Promoter

Available since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2

Plasmid

#64512

Purpose

Expression of St1 dCas9-3XTagBFP2 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XmTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV-TO

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pUC19/PLIN2 GFP-P2A-BFP

Plasmid

#202195

Purpose

knock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2

Depositor

James Olzmann

Article

Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

Insert

PLIN2 (PLIN2 Human)

Use

Cloning vector

Tags

GFP-P2A-BFP knock-in

Expression

Mutation

Promoter

lac

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 4 CMV GST mTagBFP

Plasmid

#206259

Purpose

ENTR Vector 4 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTagBFP localised to Golgi organelles under the control of CMV promoter.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

GTS mTagBFP

Use

Multimate/gateway entr 4

Tags

BFGT1 (Golgi localisation peptide)

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2

Plasmid

#212112

Purpose

Expression of C-terminally tagged GABARAP(G116A) with mTagBFP2

Depositor

Dieter Willbold

Article

Üffing et al Autophagy Reports, 3(1). doi: 10.1080/27

Insert

GABARAP(G116A)-mTagBFP2 (GABARAP Human, Entacmaea quadricolor)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

G116A (lipidation deficiency) of GABARAP

Promoter

CMV

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor-tBFP-NLS-Neo (Universal)

Plasmid

#80767

Purpose

Universal donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Insert

PITCh-gRNA#3 targeting sequence (GCATCGTACGCGTACGTGTT)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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