Addgene

We narrowed to 2,236 results for: 828

Showing: 861 - 880 of 2236 results

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MDH1-pre-let-7_PShairpin

Plasmid

#26537

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Aug 31. ():.

Insert

pre-let-7_PS hairpin

Use

Retroviral

Tags

Expression

Mammalian

Mutation

5 nt mutated to make pre-let-7 stem pairing perfe…

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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phRL-TKlk-lin-41_LCS-2U

Plasmid

#26543

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Aug 31. ():.

Insert

lin-41_LCS-2U

Use

Tags

Expression

Mammalian

Mutation

Seed regions of both target sites are mutated.

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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phRL-TKlk-lin-41_LCS-2G

Plasmid

#26544

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Aug 31. ():.

Insert

lin-41_LCS-2G

Use

Tags

Expression

Mammalian

Mutation

Seed regions of both target sites are mutated.

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
EKARet-cyto

Plasmid

#96895

Purpose

FLIM sensor for ERK activation in cytosol

Depositor

Ryohei Yasuda

Article

Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

Insert

FLIM Sensor for ERK Activity in Cytosol

Use

Tags

EGFP and sREAChet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Wnk1

Plasmid

#38779

Depositor

Melanie Cobb

Article

Xu et al J Biol Chem. 2000 Jun 2;275(22):16795-80

Insert

Wnk1 (Wnk1 Rat)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 20, 2013

Availability

Academic Institutions and Nonprofits only
pDS190

Plasmid

#34977

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

ePDZb

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pBPGUw

Plasmid

#17575

Depositor

Gerald Rubin

Article

Pfeiffer et al Tools for neuroanatomy and neurogenetics

Insert

GAL4 (GAL4 Budding Yeast)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
mNeonGreen-APEX3

Plasmid

#202590

Purpose

Gamma-retroviral vector that expresses mNeonGreen fused to APEX3.

Depositor

Reuben Harris

Article

Becker et al J Mol Biol. 2023 May 12:168145. doi: 10.

Insert

mNeonGreen-APEX3-V5 (APX1 )

Use

Retroviral

Tags

V5 epitope and mNeonGreen

Expression

Mammalian

Mutation

APEX2 L242A

Promoter

MESV LTR

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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PECAM-eGFP

Plasmid

#14688

Depositor

Victoria Bautch

Article

Kearney et al Blood. 2004 Jun 15. 103(12):4527-35.

Insert

PECAM promoter/enhancer (Pecam1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

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pAL120-TK

Plasmid

#22053

Depositor

Maria Castro

Article

King et al J Virol. 2008 May . 82(9):4680-4.

Insert

HSV Type 1 thymidine kinase

Use

Adenoviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA-M45HA

Plasmid

#17573

Depositor

Wolfram Brune

Article

Mack et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

Insert

murine cytomegalovirus M45 gene

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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pegRNA2_tevopreQ1

Plasmid

#212039

Purpose

Bacterial expression of the pegRNA for genome editing in E. coli

Depositor

Quanjiang Ji

Article

Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

Insert

pegRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

J23119

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pUAS-mCherry-NLS

Plasmid

#87695

Purpose

Gal4VP16 driven expression of mCherry-NLS in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

mCherry-NLS

Use

Tags

nuclear localization signal

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-deltaOVA

Plasmid

#64595

Purpose

Expression of N-terminally truncated chicken ovalbumin as model antigen for study of MHC class I and class II presentation

Depositor

Martin Zenke, Sandra Diebold

Article

Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

Insert

Ovalbumin

Use

Tags

Expression

Mammalian

Mutation

Lacking first 49 aa (leader sequence for transloc…

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-PANX1(HCVp)

Plasmid

#87698

Purpose

Express HCV protease-activatible pannexin-1 in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PANX(HCVp)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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CBX7

Plasmid

#25241

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

CBX7_04 (CBX7 Human)

Use

Tags

6x His and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 13, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pBAD/HisB-PhoCl

Plasmid

#87688

Purpose

Express PhoCl in E. coli

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PhoCl

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-2T_PDZ2

Plasmid

#59735

Purpose

expresses PDZ2 domain of human ZO-1 in bacteria

Depositor

Alan Fanning

Article

Fanning et al J Biol Chem. 2007 Dec 28;282(52):37710-6

Insert

zonula occludens-1 (TJP1 Human)

Use

Tags

GST and thrombin clvg

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only
pBPGw

Plasmid

#17574

Depositor

Gerald Rubin

Article

Pfeiffer et al Tools for neuroanatomy and neurogenetics

Insert

GAL4 (GAL4 Budding Yeast)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only
pES.42.1c

Plasmid

#69576

Purpose

TCR a chain cDNA in pES4 (transgenic construct) for creating OT-1 mice

Depositor

Francis R. Carbone

Article

Hogquist et al Cell. 1994 Jan 14;76(1):17-27.

Insert

TCR alpha clone 149.42 (Tcra Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

H-2Kb

Available since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only