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Plasmid#92344PurposePlasmid for expression of GFP gene with a Nicn1 gene derived Alu element containing 3'UTR.DepositorInsertNicn1 gene derived Alu element containing 3'UTR (NICN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTM-BPL
Plasmid#173766PurposeFluorescent labeling of the nuclear envelopeDepositorInsertPDGF receptor TM domain, Biotin protein ligase
UseTagsFLAG and Signal peptideExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET_RTX_(exo-)
Plasmid#102786PurposeExpresses the Reverse Transcriptase gene (3'-5' exonuclease deficient mutant)DepositorInsertreverse transcription xenopolymerase with 3'-5' deficient exonuclease
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
PBMBP-eGFP
Plasmid#40976DepositorInsertMBP promoter fragment (Mbp Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
mNeon-Vamp2
Plasmid#186619PurposeEncodes Vamp 2 fused to mNeonGreenDepositorInsertVesicle Associated Membrane Protein 2 (VAMP2 Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
YF-RhoA(CA)
Plasmid#20153DepositorInsertYFP-FKBP-RhoA(CA) (RHOA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pX330A-1x3
Plasmid#58767PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-Sec24C
Plasmid#66608PurposeYFP fusion of Sec24CDepositorInsertSec24C (SEC24C Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
ShadowG-SUMO1
Plasmid#175239PurposeBacterial expression and purification, conjugatable SUMO for SUMOylationDepositorInsertSUMO1 (SUMO1 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBad-Int Set Flipper
Plasmid#38209DepositorInsertBxb1 Integrase
UseSynthetic Biology; Biobrick cloning vectorTagsHisExpressionMutationPromoterpBAD/AraCAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBad-Xis/Int Reset Flipper
Plasmid#38207DepositorInsertsBxb1-Excisionase
Bxb1 Integrase
UseSynthetic Biology; Biobrick cloning vectorTagsExpressionMutationPromoterpBADAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
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BiP-mGFP P495L
Plasmid#62232PurposeMutant of hamster BiP-mGFP unable to bind clients.DepositorInserthamster BiP-mGFP-KDEL
UseTagsmGFPExpressionMammalianMutationPromotercmvAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-pro-siRNA-V2
Plasmid#111086PurposeFor production of recombinant siRNAs (pro-siRNAs) in E. coli.DepositorTypeEmpty backboneUseRNAiTagsExpressionBacterialMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-HA::UPRT
Plasmid#47110Purposefor subcloning UPRT into expression vectorsDepositorInserturacil phosphoribosyltransferase
UseTagsHAExpressionMutationPromoterAvailable sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMMB67EH-CFP
Plasmid#90103PurposeCFP control expression plasmidDepositorInsertCFP
UseBroad-host rangeTagsExpressionBacterialMutationPromoterPtacAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYZ462
Plasmid#187970PurposeTEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4tDepositorInsertCas9 (codon optimized for human)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterTEF1Available sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSIM24 KanR
Plasmid#67583PurposePlant Gene Expression VectorDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterM24Available sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
hRIP3 GFP wt
Plasmid#41387DepositorInsertRIP3 (RIPK3 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
Flag pLJM1 Rheb1
Plasmid#19312DepositorInsertRHEB Ras homolog enriched in brain (RHEB Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pC1-CMV-DAAO-NES
Plasmid#141107PurposeExpresses DAAO with nuclear export signal in mammalian cellsDepositorInsertDAAO-NES
UseTagsNuclear export signalExpressionMammalianMutationdeleted DAAO amino acids 367-368PromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD_Kan-LacI
Plasmid#79826PurposeArabinose induced expression of Lac repressorDepositorInsertLacI
UseTagsHis6ExpressionBacterialMutationPromoteraraBpAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Flag Rab12 wild type
Plasmid#86787Purposemammalian expression of Rab12DepositorInsertRab12 (RAB12 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330S-4
Plasmid#58780PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pU6-Lb-crRNA
Plasmid#78957PurposeCloning vector for expression of LbCpf1 crRNA. It contains BsmB1 site for cloning.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceJune 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-MPAct-mRuby3-IRES-Puro
Plasmid#155231Purposelentiviral MPAct constructDepositorInsertLentiviral expression of MPAct-mRuby sensor
UseLentiviralTagsmRuby3ExpressionMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmr101A-hVPS26
Plasmid#17636DepositorInsertVPS26 (VPS26A Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKIKOlacZCm
Plasmid#46764PurposeE.coli genomic integration at lacZDepositorTypeEmpty backboneUseE.coli genomic integrationTagsExpressionMutationPromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
Gata4-GFP donor
Plasmid#113115PurposeDonor plasmid for knock-in eGFP fusing to the c-terminal of mouse Gata4 coding sequenceDepositorInsertGata4 donor region including homology arms and eGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TrxRFP1
Plasmid#98996Purposemammalian expression of a biosensor to monitor thioredoxin redox dynamicsDepositorInserthuman thioredoxin 1, GS-rich linker, fused with a redox-sensitive RFP
UseTagsHis-tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
C1-iRFP-iLid-CaaX
Plasmid#155229Purposemembrane markerDepositorInsertiRFP C-term tagged with the ilID motif and the CaaX sequence derived from Kras4b. Membrane control
UseTagsiRFP-iLidExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-M13-TEV-C-P2A-TdTomato
Plasmid#92389Purposeexpresses Cal-light M13-TEV-C component and TdTomato in mammalian cellsDepositorInsertM13-TEV-C-P2A-TdTomato
UseTagsTdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmec-4::Chrimson::SL2::mCherry::unc-54
Plasmid#107745PurposeExpress Chrimson and mCherry in mechanosensory neurons.DepositorInsertsChrimson
mCherry
UseTagsExpressionInsect and WormMutationPromotermec-4Available sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYZ714
Plasmid#132955PurposecrRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a-Clr4 in Schizosaccharomyces pombeDepositorInsertdFnCas12a
UseCRISPR and Synthetic BiologyTagsSV40 NLS and nucleoplasmin NLSExpressionMutationPromoteradh1Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCrhaB2plus
Plasmid#164226PurposeBroad-range bacterial expression vector with modified rhamnose-inducible promoter. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterRhamnose-inducibleAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDL1
Plasmid#182263PurposeGibson cloning vector for synthesis of single and double stranded RNADepositorTypeEmpty backboneUseGibson cloning vector for synthesis of single and…TagsExpressionMutationPromoterT3 and SP6 for sense and antisense riboprobe synt…Available sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-Fn-crRNA
Plasmid#78958PurposeCloning vector for expression of FnCpf1 crRNA. It contains BsmB1 site for cloning.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceJune 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tus pGST-//
Plasmid#53305PurposeExpresses GST-tagged E.coli replication terminator protein Tus.DepositorInsertE.Coli Tus replication terminator protein
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-Cdc34a
Plasmid#18674DepositorInsertCdc34A (CDC34 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
TCRalpha-L1
Plasmid#11262DepositorInsertTCR alpha (trac Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-scFv-p65-HSF1-Blast
Plasmid#192652Purpose3rd generation lenti vector encoding scFV-p65-HSF1 with 2A Blast resistance markerDepositorInsertscFv-p65-HSF1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_Gal4LOV[SK22]-VP64-IRES-mCherry (pLZA140)
Plasmid#203907PurposeEncodes LightsOut (AsLOV2(408-543) inserted in Gal4 SK22) transcription factor in mammalian cellsDepositorInsertGal4LOV(SK22)-VP64-IRES-mCherry
UseTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSSM24: pCMV-SpuFz1
Plasmid#205260PurposeHuman expression vector for human codon optimized SpuFz1DepositorInsertSpuFz1
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PKAKTRClover
Plasmid#59146PurposeORF encoding PKA KTR mClover flanked by gateway sequencesDepositorInsertPKA Kinase Translocation Reporter (PRKACA Mouse, Human)
UseTagsExpressionMutationPromoterAvailable sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMJ326: pCMV-GtFz1
Plasmid#205264PurposeHuman expression vector for human codon optimized GtFz1DepositorInsertGtFz1
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Lifeact_STf
Plasmid#182011PurposeCMV overexpression of SNAP-tag fast in mammalian cell lines associated to actinDepositorInsertLifeact_STf
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMito-TrxRFP1
Plasmid#98997Purposemammalian expression of a biosensor to monitor thioredoxin redox dynamics, mitochondrially locaclizedDepositorInsertTrxRFP1 and mitochondrial localization sequence
UseTagsHis-tag and Mitochondria localization sequenceExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_WT
Plasmid#67721PurposeExpresses WT mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
UseTags6x-His tagExpressionBacterialMutationPromoterT7Available sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
T777T
Plasmid#113082PurposeEnhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.DepositorTypeEmpty backboneUseRNAiTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRL278
Plasmid#70256PurposesacB plasmid with polylinker; kanamycin/neomycin resistance: used as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.DepositorTypeEmpty backboneUseConditionally lethalTagsExpressionMutationPromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only