Addgene

We narrowed to 6,831 results for: 11

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pFosill-3

Plasmid

#89697

Purpose

to make Fosmids that can be converted into Illumina sequencable libraries

Depositor

Andreas Gnirke

Article

Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFosill-2

Plasmid

#89696

Purpose

to make Fosmids that can be converted into Illumina sequencable libraries

Depositor

Andreas Gnirke

Article

Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFosill-4

Plasmid

#89698

Purpose

to make Fosmids that can be converted into Illumina sequencable libraries

Depositor

Andreas Gnirke

Article

Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pB-Neo-mtkeima

Plasmid

#210338

Purpose

Overexpression of mtkeima

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

mtkeima

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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DYSF-Venus

Plasmid

#29768

Depositor

Steven Vogel

Article

Covian-Nares et al J Cell Sci. 2010 Jun 1. 123(Pt 11):1884-

Insert

DYSF-Venus (DYSF Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
FRB_LD 0_CS G_tTA-BFP

Plasmid

#58876

Purpose

MESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence

Use

AAV

Tags

EBFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pMSP1E1

Plasmid

#20062

Depositor

Stephen Sligar

Article

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1E1 (APOA1 synthetic gene)

Use

Tags

6-His

Expression

Bacterial

Mutation

"extended" MSP1; contains repeat of hel…

Promoter

Available since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pLV-GFP-Sec61β

Plasmid

#186597

Purpose

Third generation lentiviral vector expressing GFP-Sec61β

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Sec61β (SEC61B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pKAR2-Br512_Mut235

Plasmid

#179278

Purpose

Expresses Br512_Mut235 polymerase in E.coli

Depositor

Andrew Ellington

Article

Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

Insert

Engineered Bst DNA Polymerase I Large Fragment

Use

Tags

8x His tag; Villin Headpiece 47 amino acid (HP47)…

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDAI-SceI-pheS

Plasmid

#111611

Purpose

Yeast homing endonuclease I - SceI expression vector. Used for resolution of merodiploids during allelic exchange in Burkholderia sp.

Depositor

Tim Tolker-Nielsen

Article

Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

Insert

pheS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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plk1 T210D

Plasmid

#132964

Purpose

To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

Depositor

Michael Yaffe

Article

Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

Insert

polo-like kinase 1 (PLK1 Human)

Use

Tags

Expression

Mammalian

Mutation

T210D

Promoter

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-C2gamma

Plasmid

#21215

Depositor

Tobias Meyer

Article

Codazzi et al Curr Biol. 2001 Jul 24. 11(14):1089-97.

Insert

GFP-pcDNA3-PKCgamma-c2 (Prkcg Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

The C2-domain from PKCgamma (aa170-260) plus flan…

Promoter

Available since

July 13, 2009

Availability

Academic Institutions and Nonprofits only

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pLKO.1-scrCdh20.1 GFP

Plasmid

#209106

Purpose

GFP expressing scramble of shRNA targeting Cdh20

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCdh20.1

Use

Tags

Expression

Mutation

Promoter

Available since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pDBTrp-LexABD-CRY2FL

Plasmid

#78210

Purpose

Use with Addgene 40615 for blue light transcription control in yeast

Depositor

Chandra Tucker

Article

Pathak et al ACS Synth Biol. 2014 Nov 21;3(11):832-8.

Insert

LexABD-CRY2 (CRY2 Mustard Weed)

Use

Tags

lexA binding domain

Expression

Yeast

Mutation

contains endogenous CRY2 NLS

Promoter

ADH1

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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p3259 pCMV hUBC9 mt HA

Plasmid

#14439

Depositor

Peter Howley

Article

Yasugi et al Nucleic Acids Res. 1996 Jun 1. 24(11):20

Insert

UBC9 C93S (UBE2I Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Cys93Ser mutation (TGC changed to AGC)

Promoter

CMV

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pKD4-Nter-APEX2

Plasmid

#112868

Purpose

Bacterial plasmid derived from pKD4 for chromosomal insertion of APEX2 gene in 5' of a gene of interest by using Datsenko & Wanner method

Depositor

Eric Cascales

Article

Santin et al Nat Microbiol. 2018 Nov;3(11):1304-1313.

Insert

APEX2

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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plk1 T210A

Plasmid

#132965

Purpose

To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

Depositor

Michael Yaffe

Article

Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

Insert

polo-like kinase 1 T210A (PLK1 Human)

Use

Tags

Expression

Mammalian

Mutation

T210A

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLH-sgRNA-Sirius-4X(MS2-PP7)

Plasmid

#121941

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-4X(MS2-PP7)

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Mutation

Promoter

human U6 promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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WT lamin A minigene

Plasmid

#20291

Depositor

Tom Misteli

Article

Scaffidi et al Nat Med. 2005 Apr . 11(4):440-5.

Insert

lamin A (LMNA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pIntegrase_2

Plasmid

#60574

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_2

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pPLtetO-ClpX

Plasmid

#89657

Purpose

Plasmid for titrating synthesis of ClpX with doxycycline

Depositor

Uwe Sauer

Article

Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

Insert

clpX

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLH-sgRNA-Sirius-8XPP7

Plasmid

#121940

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-8XPP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Mutation

Promoter

human U6 promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5-Flag-RhoA wt

Plasmid

#11750

Depositor

Jeff Wrana

Article

Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

Insert

RhoA (RHOA Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
ER50-SpCas9-ER50

Plasmid

#85448

Purpose

Expresses SpCas9 fused to ER50 domain on both N- and C-termini in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

ER50-SpCas9-ER50

Use

AAV

Tags

destabilized domain of estrogen receptor ligand b…

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459V2.0-HypaCas9-mRuby2

Plasmid

#183872

Purpose

pX459V2.0 plasmid expressing HypaCas9 with P2A-mRuby2 and T2A-Puro, with a sgRNA cloning site for CRISPR editing in mammalian cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Type

Empty backbone

Use

CRISPR

Tags

HypaCas9-P2A-mRuby2-T2A-Puro

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 CDK7 HA (P#633)

Plasmid

#14647

Depositor

Matija Peterlin

Article

Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

Insert

Cdk7 (CDK7 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
AcpP pet29a C-terminal His Tag

Plasmid

#75016

Purpose

Expresses the acyl carrier protein (ACP) from the E. coli fatty acid synthase

Depositor

Michael Burkart

Article

Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

Insert

AcpP (acpP E. coli)

Use

Tags

His Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
pCS2+ Dkk1-flag

Plasmid

#16690

Depositor

Xi He

Article

Semënov et al Curr Biol. 2001 Jun 26. 11(12):951-61.

Insert

Dkk1 (DKK1 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Expression

Mutation

Promoter

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ910

Plasmid

#44481

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, NeoR, MCS

Use

Multiple cloning site vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
DHFR-PP7-VP64_T2A_GFP

Plasmid

#86167

Purpose

Expresses PP7-VP64 fused to DHFR domain on N-terminus in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

DHFR-PP7-VP64_T2A_GFP

Use

CRISPR and Lentiviral

Tags

Destabilized domain (N-terminus version) of E.col…

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 CDK8 HA (P#634)

Plasmid

#14649

Depositor

Matija Peterlin

Article

Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

Insert

Cdk8 (CDK8 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
DYSF-3HA

Plasmid

#29767

Depositor

Steven Vogel

Article

Covian-Nares et al J Cell Sci. 2010 Jun 1. 123(Pt 11):1884-

Insert

DYSF (DYSF Human)

Use

Tags

3xHA

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1/VChR1-EYFP

Plasmid

#20955

Purpose

Mammalian expression of VChR1 fused to EYFP, for neuronal excitation

Depositor

Karl Deisseroth

Article

Zhang et al Nat Neurosci. 2008 Jun . 11(6):631-3.

Insert

volvox channelrhodopsin-1

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pC0096 RESCUE truncation (Cas13 delta 892-1095)

Plasmid

#130663

Purpose

CMV-dCas13b6(delta 892-1095)-mapkNES-GS-RESCUE

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Insert

dRanCas13b(delta 892-1095)-ADAR2dd

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
FL-BCAN-lentiviral-GFP

Plasmid

#29704

Depositor

Mariano Viapiano

Article

Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

Insert

Brevican (BCAN Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-R4-uAb

Plasmid

#101800

Purpose

Mammalian cell expression of the beta-galactosidase-specific ubiquibody, R4-uAb

Depositor

Matthew DeLisa

Article

Portnoff et al J Biol Chem. 2014 Mar 14;289(11):7844-55

Insert

R4-uAb

Use

Tags

6xHis and Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ909

Plasmid

#44480

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, unc-119, MCS

Use

Multiple cloning site vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
UbG76V-eGFP-V5His_20aa

Plasmid

#23970

Depositor

Nico Dantuma

Article

Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

Insert

Ub-G76V-eGFP-V5HIS_20aa

Use

Tags

eGFP

Expression

Mammalian

Mutation

G76V mutation in Ubiquitin. Ub fused to N-termin…

Promoter

Available since

March 22, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSC236

Plasmid

#216368

Purpose

xhNup155-Nb2i (+2Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup155-Nb2i (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSC235

Plasmid

#216372

Purpose

xhNup155-Nb3i (+2Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup155-Nb3i (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSC227

Plasmid

#216351

Purpose

xhNup35-Nb1t (+3Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup35-Nb1t (+3Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCJH002_10xHis-MBP-TEVcs-Cas12c(4)_Amp

Plasmid

#183069

Purpose

Bacterial protein expression plasmid of wild-type Cas12c_4. This is a R965H version of the Cas12c in Harrington et al., 2020.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c_4

Use

CRISPR

Tags

His10 and MBP

Expression

Bacterial

Mutation

Wild-type

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101103

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to VEGFR1

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

VEGFR1-dCas9(C)

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_WTDHFR_WTTS

Plasmid

#91226

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-syn-fRCaMP2

Plasmid

#125246

Purpose

Expresses red fluorescent calcium sensor in hippocampal slices

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRCaMP2

Use

Tags

Expression

Mammalian

Mutation

D396A

Promoter

synapsin

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-HA-rat Nrxn3beta AS4(-)

Plasmid

#58269

Purpose

Expression plasmid for HA-tagged rat Neurexin 3beta, lacking the insert at alternatively spliced segment 4 (AS4-)

Depositor

Peter Scheiffele

Article

Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Insert

Nrxn3beta AS4(-) (Nrxn3 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pNAS1B split mCherry N-link/link-C

Plasmid

#61965

Purpose

Negative control duet plasmid for split mCherry assembly assay (i.e., neither half of mCherry is fused to another peptide or protein)

Depositor

Lynne Regan

Article

Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

Inserts

N-mCherry-link
link-C-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

PBAD and PLtetO

Available since

Feb. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBlastR-12xLexAOp-mCherry-MiniWhite

Plasmid

#165917

Purpose

Blasticidin resistant 12xLexAOp mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable LexAOp Response Vector

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFBD-ESCRT 0

Plasmid

#21499

Depositor

James Hurley

Article

Ren et al Structure. 2009 Mar 11. 17(3):406-16.

Insert

pFastBac Dual-His/MBP/Hrs-GST/STAM1 (STAM Human, Mouse)

Use

Tags

GST and His-MBP

Expression

Insect

Mutation

none

Promoter

Available since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
mNeptune-pBAD

Plasmid

#54714

Purpose

Localization: Protein Expression Vector , Excitation: 600, Emission: 650

Depositor

Roger Tsien, Nathan Shaner, Michael Davidson, Robert Campbell

Article

Lin et al Chem Biol. 2009 Nov 25;16(11):1169-79. d

Type

Empty backbone

Use

Tags

6xHis and mNeptune

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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