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Plasmid#46447DepositorInsertJak1 (JAK1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 430-552PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Abl-SH2 RK
Plasmid#46403DepositorInsertAbl RK (Abl1 Mouse)
UseTagsGST and Thrombin siteExpressionBacterialMutationcontains amino acids 139-239PromoterTacAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-92a-1-5p
Plasmid#103750PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-92a-1-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-92a-1-5p target (MIR92A1 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1-IKGRQtoDSYAA-shRNAres_W
Plasmid#147900PurposeMammalian Expression of HsNot1iso1-IKGRQtoDSYAA-shRNAresDepositorInsertHsNot1iso1-IKGRQtoDSYAA-shRNAres (CNOT1 Human)
UseTagsGFP (G4C mutation)ExpressionMammalianMutationNM_016284.4, I1423D, K1426S, G1451Y, R1458A, and…PromoterAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-GOLPH3
Plasmid#21688DepositorInsertGolgi Phosphoprotein 3 (GOLPH3 Human)
UseGateway entry cloneTagsExpressionMutationlast nucleotide of stop codon removed for c-termi…PromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pX330-Esrrb gRNA
Plasmid#128841PurposegRNA for targeting mouse Esrrb locus using CRISPR-cas techniqueDepositorInsertEsrrb gRNA (Esrrb Mouse)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGLS3-5xHRE-p53(K120R)
Plasmid#72554PurposeHypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K120R.DepositorInsertp53 (TP53 Human)
UseTags5XHREExpressionMammalianMutationK120RPromoterE1B minimal promoterAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX Grb10-SH2
Plasmid#46437DepositorInsertGrb10 (GRB10 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 435-548PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSc2
Plasmid#80437PurposeExpresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cellDepositorInsertssp Cas9 gRNA
eGFP
UseCRISPRTagsExpressionMammalianMutationPromoterCMV and U6Available sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBullet-cg-c
Plasmid#53070Purposedestination vector with cis-golgi marker (alpha-man I 49AA) -ECFP for tagged fluorescent protein (C-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTBL681 CHYRON4 integration construct
Plasmid#126449PurposeTo integrate the CHYRON4 locus at AAVS1 in human cells.DepositorInsertspU6/3xLacO-CHYRON4 hgRNA
pCMV-puro
UseTagsExpressionMammalianMutationThe SpCas9 sgRNA constant region is mutated to ma…PromoterCMV and human U6/3xLacOAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET41s-GST-ATF6a-AD
Plasmid#64774Purposebacterial expression of GST-tagged ATF6 (1-150)DepositorInsertATF6a-AD (1-150) (ATF6 Human)
UseTagsGSTExpressionMammalianMutationC-term deletionPromoterAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX RASGAP(C)-SH2
Plasmid#46431DepositorInsertGap/RASGAP(C) (RASA1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 339-452PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTwist-FLAG-SRSF3_Truncated_Codon.Optimized
Plasmid#172346PurposeExpression of FLAG-tagged, codon-optimized, truncated SRSF3DepositorInsertserine and arginine rich splicing factor 3 (SRSF3 Human)
UseTagsFLAGExpressionMammalianMutationcodon-optimized, truncated at amino acid 114 with…PromoterEF-1aAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-EE-UdgX-NG-nCas9
Plasmid#163570PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-EE-UdgX-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…PromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 - HA-KLF4 FL delta K5S
Plasmid#34594DepositorInsertKLF4 (KLF4 Human)
UseTagsHA tagExpressionMammalianMutationdeletion of K5S, corresponding to bases 1781 to 2…PromoterCMVAvailable sinceJan. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX RASGAP(NC)-SH2
Plasmid#46433DepositorInsertGap/RASGAP(NC) (RASA1 Human)
UseTagsGSTExpressionBacterialMutationcontains amino acids 171-448PromoterTacAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV_RBMX-eA3A-UdgX-HF-nCas9
Plasmid#163548PurposeMammalian CG-to-GC base editingDepositorInsertRBMX-eA3A-UdgX-HF-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)PromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX Nsp2-SH2
Plasmid#46460DepositorInsertNsp2 (BCAR3 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 144-259PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGL3-DICER-Prom Ebox double mut
Plasmid#25854DepositorInsertDICER-Promoter Ebox double mut (DICER1 gene ID 23405, Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2010AvailabilityAcademic Institutions and Nonprofits only