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Plasmid#174881PurposeTargeting vector for generating Flnc STREAMING-tag KI cellDepositorInsertSTREAMING-tag with homology arms for Flnc
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTV-Usp5-5prime-1000-24MS96T-3F_NeoR
Plasmid#174885PurposeTargeting vector for generating Usp5 STREAMING-tag KI cellDepositorInsertSTREAMING-tag with homology arms for Usp5
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTV-Wnk1-5prime-1000-24MS96T-3F_NeoR
Plasmid#174886PurposeTargeting vector for generating Wnk1 STREAMING-tag KI cellDepositorInsertSTREAMING-tag with homology arms for Wnk1
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Chinchilla–insM
Plasmid#191226PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationremoved methionine at 122 in the protein sequencePromoterPH and p10Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sandgrouse–insD
Plasmid#191228PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationremoved aspartic acid at 121 in the protein seque…PromoterPH and p10Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 Ta-SRO1 JN177
Plasmid#192546PurposepENTR plasmid Triticum aestivum SRO1, full CDS, cultivar JN177, no stop codonDepositorInsertSIMILAR TO RCD ONE 1 (SRO1), cultivar Jinan 177 (JN177)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 Ta-sro1 SR3
Plasmid#192547PurposepENTR plasmid Triticum aestivum sro1, full CDS, cultivar SR3, no stop codonDepositorInsertSIMILAR TO RCD ONE 1 (SRO1), cultivar Shanrong No. 3 (SR3)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACE-K2P4.1-StrepTagII
Plasmid#191474PurposeExpresses K2P4.1 with C-terminal StrepTagIIDepositorInsertK2P4.1 (KCNK4 Human)
UseTagsExpressionInsectMutationN104Q N108QPromoterpolHAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265N2
Plasmid#174001PurposeGateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-UNG for C-G base editingDepositorInsertrAPOBEC1-zCas9(D10A)-SpRY-UNG
UseCRISPRTags3X FLAG, NLSExpressionPlantMutationIn zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …PromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265O2
Plasmid#174002PurposeGateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1 for C-G base editingDepositorInsertrAPOBEC1-zCas9(D10A)-SpRY-rXRCC1
UseCRISPRTags3X FLAG, NLSExpressionPlantMutationIn zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …PromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131-RZ-Cas12j2
Plasmid#173927PurposeGolden Gate entry vector; empty vector to clone the 1st Cas12j2 crRNA with ribozyme processingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132-RZ-Cas12j2
Plasmid#173928PurposeGolden Gate entry vector; empty vector to clone the 2nd Cas12j2 crRNA with ribozyme processingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133-RZ-Cas12j2
Plasmid#173929PurposeGolden Gate entry vector; empty vector to clone the 3rd Cas12j2 crRNA with ribozyme processingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134-RZ-Cas12j2
Plasmid#173930PurposeGolden Gate entry vector; empty vector to clone the 4th Cas12j2 crRNA with ribozyme processingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265L2
Plasmid#174000PurposeGateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY for C-G base editingDepositorInsertUNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY
UseCRISPRTags3X FLAG, NLSExpressionPlantMutationIn rAPOBEC1: R33A; in zCas9(D10A)-SpRY: D10A, A61…PromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet1-6xHis-TEV-OPTN
Plasmid#190192PurposePlasmid for the expression of 6xHis-TEV-OPTN. Internal reference: SMC397DepositorInsertoptineurin (OPTN Human)
UseTags6xHis and TEVExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv6_dPC_2xChromo
Plasmid#179398PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of dPC_2xChromoDepositorInsertdPC_2xChromo
UseTagsAviTag and EGFPExpressionMammalianMutationPromoterCAGGSAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEVA251_ Ptrc.x.tetO2::D-HicDH
Plasmid#185456PurposeCDS of D-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 under the control of the synthetic promoter Ptrc.x.tetO2 and the RBS BBa_B0030 in pSEVA251 plasmid.DepositorInsertD-HicDH from Lactobacillus paracasei DSM 20008
UseTagsExpressionBacterialMutationPromoterPtrc.x.tetO2Available sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FluoSTEP-AKAR (C/Y)
Plasmid#181862PurposeFluoSTEP-AKAR using split CFP and cpVenus(E172) as the FRET donor and acceptor. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-AKAR (C-Y)
UseTagsCFP(1-10) and cpVenus(E127)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-ZNF267
Plasmid#178452Purposedoxycycline-inducible expression of Human ZNF267 in mammalian cellsDepositorInsertZNF267 (ZNF267 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only