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Plasmid#170137PurposeAAV vector carrying ADAR2-DDC(E488Q)-λN-P2A-MCP-ADAR2-DDN with a nuclear export signalDepositorInsertADAR2-DDC(E488Q)-λN-P2A-MCP-ADAR2-DDN
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TWIN-STREP-FLAG-NPRL3
Plasmid#164984PurposeNPRL3 expressing vector for mammalian cellsDepositorInsertNPRL3 (NPRL3 Human)
UseTags2xSTREP FLAGExpressionMammalianMutationPromoterCAGAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
PBLO CasX-gRNA
Plasmid#126419PurposeE. coli vector for CasX gRNA --- Used for In Vitro TranscriptionDepositorInsertcasX gRNA
UseCRISPR; In vitro transcriptionTagsExpressionMutationPromoterAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
SF-U7snRNA-CypA-E4
Plasmid#190695PurposeControl vector expressing a U7-based antisense oligonucleotide targeting CypA exon 4DepositorInsertU7 snRNA antisense CypA Exon 4
UseLentiviralTagsExpressionMammalianMutationPromoterU7 promoterAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTU425
Plasmid#178984PurposepACYC containing Candidatus “Scalindua brodae” TPR-CHAT H585A C627A (codon-optimized)DepositorInsertTPR-CHAT H585A C627A
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-4493NES
Plasmid#175811PurposeThe lentiviral vector for Booster-PKA, a Red-Shifted genetically encoded FRET biosensor for monitoring PKA activity.DepositorInsertBooster-PKA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO HIS DNAJB9
Plasmid#19559DepositorInsertDnaJ (Hsp40) homolog, subfamily B, member 9 (DNAJB9 Human)
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 UBXN2A
Plasmid#113505PurposeBacterial expression of GST-tagged UBXN2ADepositorInsertUBXN2A (UBXN2A Human)
UseTagsGST-Thrombin cleavage siteExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
NG0750 pCI-TPI-PTC48-4H
Plasmid#65803PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
UseTags4H (4 copies of short beta-globin 3'UTR for …ExpressionMammalianMutationpremature stop codon at 48PromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
myc-hIFITM3-S61A,N64A,T65A
Plasmid#104362PurposeExpresses human IFITM3-S61A,N64A,T65A with an N-terminal myc tag in mammalian cells.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsmycExpressionMammalianMutationmutated the following amino acids: S61A,N64A,T65APromoterCMVAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pco-vCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10
Plasmid#197966PurposeT-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by CmYLCVp, flanked by ribozymes.DepositorInsertspco-vCasphi-2
AtPDS3 gRNA10
UseCRISPRTagsExpressionPlantMutationPromoterCmYLCVp and pUBQ10Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-LKB1(KD)
Plasmid#84640PurposemCherry-tagged, LKB1(K78I); kinase-dead mutantDepositorInsertmCherry-LBK1(K78I) (STK11 Synthetic, Human)
UseTags6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …ExpressionMammalianMutationLysine 78 mutated to Isoleucine; kinase deadPromoterCMVAvailable sinceMay 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
ZIKV_Trigger_27B
Plasmid#75008PurposePortion of Zika Virus genome (KU312312: 1348-1494) containing sensor 27B trigger sequenceDepositorInsertZIKV_Trigger_27B
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEntry_SARS-CoV-1-Spike
Plasmid#168864PurposeGateway-compatible Entry vectorDepositorInsertSARS-CoV-1-Spike (S )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-Mac 3.0-EYFP
Plasmid#35515PurposeLentiviral expression of CaMKIIa-driven Mac 3.0 for optical inhibition.DepositorInserteMac 3.0-EYFP
UseLentiviralTagsEYFPExpressionMammalianMutationTrafficking signal, ER exportPromoterCaMKIIaAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-H2B.APEX-P2A-EGFP
Plasmid#182825PurposeCre-dependent expression of H2B-APEX fusionDepositorInsertH2B.APEX-P2A-EGFP
UseAAVTagsEGFP reporter (P2A)ExpressionMutationPromoterEF1aAvailable sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-NlaCascade-NLS-6xHis
Plasmid#180213PurposeExpressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.ColiDepositorInsertNlaCas5-Cas8-Cas7
UseTags6XHis and NLSExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGS1034
Plasmid#63796PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal CBP-3xFLAG-TEV-ProtA triple tag and a LEU2 selection markerDepositorInsertCBP-3xFLAG-TEV-Protein A followed by KlLEU2
UseE. coli cloning vectorTagsCBP-3xFLAG-TEV-Protein AExpressionMutationPromoternoneAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER21-puro_TAOK1
Plasmid#209777Purposedoxycycline-inducible expression of TAOK1DepositorInsertTAOK1 (TAOK1 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SHANK3-1/1
Plasmid#91421PurposeProtein expression and purification of human SH3 domain construct SHANK3-1/1DepositorInsertSHANK3-1/1 (SHANK3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only