-
Plasmid#52636Purposemammalian expression of HA tagged HIF1alpha (full length) with P402A,P564A mutationDepositorInsertHIF1alpha P402A, P564A (HIF1A Human)
UseTagsHAExpressionMammalianMutationP402A, P564APromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRRL WS1.6 WASp
Plasmid#36250DepositorInsertWASp (WAS Human)
UseLentiviralTagsExpressionMutationPromoterWS1.6Available sinceJuly 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCI HA NEDD4
Plasmid#27002DepositorInsertNEDD4 (NEDD4 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pegCTNNB1 S45del
Plasmid#169844PurposepegRNA used for installation (via TCC deletion) of the oncogenic S45del in Ctnnb1 in mouse liver.DepositorInsertpegCTNNB1 S45DEL (Ctnnb1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mBeRFP
Plasmid#175173Purposelarge-stokes shift red fluorescent protein mBeRFPDepositorInsertmBeRFP
UseTagsExpressionMutationPromoterAvailable sinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDF-Frb-v1
Plasmid#201923PurposeE. coli vector expressing FrbA, FrbB, FrbC, FrbD and FrbE for the biosynthesis of non-hydrolyzable phosphoserine from phosphoenolpyruvate.DepositorInsertsFrbA
FrbB
FrbC
FrbD
FrbE
UseTagsExpressionBacterialMutationPromoterT7 (modified), T7 (modified, moderate), T7 (modif…Available sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL1777 (pEffector)
Plasmid#160731PurposeEffector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.DepositorInsertVchCAST proteins and crRNA
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
PHP.AX
Plasmid#195218PurposeAAV capsid for broadly targeting cell types in the CNS (neurons including both excitatory and inhibitory neurons, astrocytes)DepositorInsertSynthetic construct isolate AAV-PHP.AX VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH21-BNA-Luc
Plasmid#187924PurposeExpresses Luciferase reporter RNA construct under the 5' and 3' UTRs of NA segment of Influenza B/Brisbane/60/2008 virus by RNA polymerase I in mammalian cellsDepositorInsertReporter RNA (RNA encoding firefly luciferase under the viral promoter of segment 6 of Influenza B/Brisbane/60/2008 virus)
UseTagsExpressionMammalianMutationPromoterRNA polymease I PromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
L13-Arl13bGFP
Plasmid#40879Purpose3rd generation transfer vectorDepositorInsertArl13b (Arl13b Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1
Plasmid#118593PurposeExpresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoterDepositorInsertPuro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1Alpha/TRE3GAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGreenIIM DR5v2-ntdTomato/DR5-n3GFP
Plasmid#61628PurposeExpresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoterDepositorInsertsntdTomato
n2xEGFP
UseTagsExpressionPlantMutation2 copies of EGFP with N-terminal SV40 NLSPromoterAuxin-dependent DR5 promoter containing 9 reverse…Available sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-F-RAM-d2tTA-TRE-mKate2
Plasmid#140274PurposeF-RAM, the Fos-dependent reporterDepositorInsertmKate2
UseAAVTagsExpressionMutationPromoterTREAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE-60NA-msGFP2
Plasmid#135301PurposeEncodes a photostable monomeric superfolder GFPDepositorInsertmsGFP2
UseTagsExpressionBacterialMutationPromoterT5Available sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBR_attB(bxb)_ccdB_lox_v2
Plasmid#198040PurposeBxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)DepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
HDAC7 Flag
Plasmid#13824PurposeMammalian expression of human histone deacetylase 7 with flag tagDepositorInsertHDAC7 (HDAC7 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDEST-eGFP-C1-VPS35
Plasmid#163622Purposehuman VPS35 cDNA C-terminally tagged with eGFPDepositorInserthuman VPS35 (VPS35 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAurora2
Plasmid#213134PurposeIntegrated transcriptional and translational control for stringently blue-light-activated gene expressionDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
piRFP670-N1
Plasmid#45457DepositorHas ServiceCloning Grade DNAInsertiRFP670
UseTagsExpressionMammalianMutationT2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…PromoterCMVAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHRIG-Akt1
Plasmid#53583Purposelentiviral expression of constitutively active human Akt1 and EGFPDepositorInsertcAkt1 (AKT1 Human)
UseLentiviralTagsHA and IRES-EGFPExpressionMammalianMutationconstitutively active (myristoylated version)PromoterCMVAvailable sinceOct. 28, 2014AvailabilityAcademic Institutions and Nonprofits only