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Plasmid#104403Purposetransient expression of dCas9-DNMT3a fusion proteinDepositorInsertDNMT3a (DNMT3A Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBLO43.3.-6_human ProCas9Flavi-6
Plasmid#121630PurposeU6-sgRNAdest_CMV-intron_hProCas9Flavi-6_T2A Mcherry_AmpR_ColE1DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterU6Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH22
Plasmid#125808PurposeLevel0 xCas9 3.7DepositorInsertxCas9 3.7
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTFAP2C-donor
Plasmid#113824PurposeCRISPR donor plasmid to tag human transcription factor TFAP2C with GFPDepositorInsertTFAP2C homology arms flanking EGFP-IRES-Neo cassette (TFAP2C Human)
UseCRISPRTagsExpressionMutationrs6025024, hg38:20:56638660:TTT/-PromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBLO5.01
Plasmid#121418PurposepUC19_BsaI-dCas9-BsaIDepositorInsertdcas9
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJW1259
Plasmid#61251PurposeCas9 plasmid with sgRNA deleted; for use in C. elegansDepositorInsertDeletion
UseCRISPRTagsExpressionWormMutationDeleted sgRNA(F+E) sequence from pJW1219PromoterAvailable sinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pARNTL-donor
Plasmid#112384PurposeCRISPR donor plasmid to tag human transcription factor ARNTL with GFPDepositorInsertARNTL homology arms flanking EGFP-IRES-Neo cassette (BMAL1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pISL1-donor
Plasmid#113820PurposeCRISPR donor plasmid to tag human transcription factor ISL1 with GFPDepositorInsertISL1 homology arms flanking EGFP-IRES-Neo cassette (ISL1 Human)
UseCRISPRTagsExpressionMutationUnknownPromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBLO 9.2_bCas9-CP199
Plasmid#121496PurposeTetR_tetR/tetA promoters_dCas9-CP_CM_P15ADepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterp15AAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRB1083
Plasmid#71480PurposeExpresses VRER Cas9 variant with eef-1A.1 (eft-3) promoterDepositorInsertVRER Cas9 variant
UseCRISPRTagsExpressionWormMutationPromotereft-3 (eef-1A.1)Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Construct 3 - U6p_20nt1_mod2_sgRNA (stgRNA)
Plasmid#81246PurposeFor assaying self-targeting activity via Cas9DepositorInsertsgRNA
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFH76
Plasmid#125815PurposeLevel0 ScCas9, wheat codon optimizedDepositorInsertScCas9, wheat codon optimized
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH80
Plasmid#128191PurposeLevel1 ZmUBIp::xCas9 3.7:NOStDepositorInsertZmUBIp::xCas9 3.7:NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
Construct 1 - U6p_20nt1_WT_sgRNA
Plasmid#81245PurposeFor assaying self-targeting activity via Cas9DepositorInsertsgRNA
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDDIT3-donor
Plasmid#112300PurposeCRISPR donor plasmid to tag human transcription factor DDIT3 with GFPDepositorInsertDDIT3 homology arms flanking EGFP-IRES-Neo cassette (DDIT3 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEX-A-U6-MaSgRNA_PuroR
Plasmid#84780PurposeExpresses major satellite-specific sgRNA.DepositorInsertmajor satellite-specific sgRNA
UseCRISPRTagsExpressionBacterial and MammalianMutationPromoterU6Available sinceJuly 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHOXB5-donor
Plasmid#113815PurposeCRISPR donor plasmid to tag human transcription factor HOXB5 with GFPDepositorInsertHOXB5 homology arms flanking EGFP-IRES-Neo cassette (HOXB5 Human)
UseCRISPRTagsExpressionMutationrs2288276, rs7406798, rs36049616, rs3087996PromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBLO 9.6_bCas9-CP1010
Plasmid#121498PurposeTetR_tetR/tetA promoters_dCas9-CP_CM_P15ADepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterp15AAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBLO 9.15_bCas9-CP1249
Plasmid#121500PurposeTetR_tetR/tetA promoters_dCas9-CP_CM_P15ADepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterp15AAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBLO 9.3_bCas9-CP230
Plasmid#121497PurposeTetR_tetR/tetA promoters_dCas9-CP_CM_P15ADepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterp15AAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only