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Plasmid#90405PurposeSTAT5 - Stat5 gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterSIEAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
LD-GA
Plasmid#209862PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4DepositorInsertddGFP A
UseTagsHairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMIG-FLAG-MLL-AF9
Plasmid#71443PurposeRetroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-GFPDepositorInsertMLL-Af9 (KMT2A Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterMSCVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hM4D(Gi)-mCherry
Plasmid#50475PurposeGi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.DepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InserthM4D(Gi)-mCherry
UseAAVTagsmCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7 RNA POL
Plasmid#138352PurposeExpressing T7 RNA Polymerase in mammalian cellsDepositorInsertT7 RNA POL
UseCRISPR and LentiviralTagsNoExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a-CysRS-His
Plasmid#124107PurposeBacterial expression plasmid for PURE system componentsDepositorInsertCysteinyl-tRNA ligase (cysS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag mTOR wt
Plasmid#26603PurposeMammalian expression of human flag-tagged mTorDepositorInsertmTOR (MTOR Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
lenti sgRNA(MS2)_zeo backbone
Plasmid#61427Purpose3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and EF1AAvailable sinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-8xHis-Ub
Plasmid#107392PurposeMammalian expression 8xHis-UbDepositorInsert8 tandem repeats of 6xHis-Ub (See comments below)
UseTagsNoneExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -