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pLV hUbC-Cas9-T2A-GFP

Plasmid

#53190

Purpose

3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized Cas9 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pBS-ldhGFP

Plasmid

#27170

Depositor

Michela Lizier

Article

Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

Insert

lactate dehydrogenase promoter region fused with modified green fluorescent protein 5

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pIB184 (EM)

Plasmid

#90194

Purpose

E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

Depositor

Indranil Biswas

Article

Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

Type

Empty backbone

Use

Tags

None

Expression

Bacterial

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-dCas9-KRAB-PGK-HygR

Plasmid

#83890

Purpose

Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

humanized dead Cas9 KRAB
aminoglycoside phosphotransferase from E. coli

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mutation

D10A and H840A

Promoter

Human Ubiquitin C Promoter and mouse phosphoglyce…

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-GAL9

Plasmid

#166689

Purpose

For creating a stable mCherry-GAL9 cell line

Depositor

Alan Sabirsh

Article

Munson et al Commun Biol. 2021 Feb 16;4(1):211. doi:

Insert

LGALS9 (LGALS9 Human)

Use

Synthetic Biology

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-STING-sh5

Plasmid

#127647

Purpose

Knock-down of human STING

Depositor

Nicolas Manel

Article

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

STING shRNA (STING1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-VP40

Plasmid

#74421

Purpose

Mammalian expression of Ebolavirus matrix protein VP40 with an mCherry fusion

Depositor

Judith White

Article

Shoemaker et al PLoS One. 2013;8(2):e56265. doi: 10.1371

Insert

VP40

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pET21a Ov-iPGM

Plasmid

#180245

Purpose

Bacterial expression plasmid for production of recombinant Onchocerca volvulus iPGM His10

Depositor

James Inglese

Article

Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

Insert

Onchocerca volvulus iPGM-10His

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pET21a Wb-iPGM

Plasmid

#180246

Purpose

Bacterial expression plasmid for production of recombinant Wuchereria bancrofti iPGM His10

Depositor

James Inglese

Article

van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

Insert

Wuchereria bancrofti iPGM-10His

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a Ce-iPGM-bio

Plasmid

#162559

Purpose

Expresses C. elegans iPGM containing a C-terminal biotinylation site and His tag

Depositor

James Inglese

Article

Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

Insert

C. elegans iPGM bio-6His (ipgm-1 Nematode)

Use

Tags

6His tag, T7 tag, Thrombin cleavage site, and bio…

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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