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Plasmid#132434PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertTSHZ1 (TSHZ1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR_Wheat_live_Cas9
Plasmid#126880PurposeGateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and BpiI sites for sgRNA modules.DepositorInsertWheat_live_Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-RNF125 Ring Mutant
Plasmid#122046Purposeexpress RNF125 RMDepositorInsertRNF125 RM (RNF125 Human)
UseTagsFlagExpressionMammalianMutationRing mutant C37A, C40APromoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
MoClo adapted pUC57-Kan Level 2
Plasmid#109218PurposeNon binary level 2 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 TCERG1L WW domain #2
Plasmid#104281PurposeBacterial expression of WW domain #2 from TCERG1LDepositorInsertTCERG1L WW domain #2 (TCERG1L Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 STXBP4 WW domain
Plasmid#104265PurposeBacterial expression of WW domain from STXBP4DepositorInsertSTXBP4 WW domain (STXBP4 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 PIN1L WW domain
Plasmid#104269PurposeBacterial expression of WW domain from PIN1LDepositorInsertPIN1L WW domain (PIN1P1 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
mr-GST N-term fusion
Plasmid#112983PurposeFor protein expression and purification of N-terminus of Drosophila mr (APC2)DepositorInsertmr (APC2) (mr Fly)
UseTagsExpressionBacterialMutationN-term onlyPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 SMURF1 WW domain #1
Plasmid#104237PurposeBacterial expression of WW domain #1 from SMURF1DepositorInsertSMURF1 WW domain #1 (SMURF1 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceMarch 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 SMURF1 WW domain #2
Plasmid#104238PurposeBacterial expression of WW domain #2 from SMURF1DepositorInsertSMURF1 WW domain #2 (SMURF1 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceMarch 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
DsRed-CPI-17-T38A-NES
Plasmid#100791PurposeMammalian expression of Fluorescent CPI-17 neutral mutation cytosolicDepositorInsertPPP1R14A (PPP1R14A Human)
UseTagsDsRedExpressionMammalianMutationT38APromoterCMVAvailable sinceNov. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-SIRPT1-Xenopus (Silurana) tropicalis
Plasmid#75323PurposeGST fusion Xenopus (Silurana) tropicalis Sacsin 87-1376DepositorInsertSacsin Xenopus (Silurana) tropicalis 87-1376
UseTagsGST-TagExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-SIRPT1-Ceratotherium simum simum
Plasmid#75331PurposeGST fusion Ceratotherium simum simum Sacsin 89-1366DepositorInsertSacsin Ceratotherium simum simum 89-1366
UseTagsGST-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-SIRPT1-Mustela putorius furo
Plasmid#75333PurposeGST fusion Mustela putorius furo Sacsin 94-1381DepositorInsertSacsin Mustela putorius furo 94-1381
UseTagsGST-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pColdII-SIRPT1-Xenopus (Silurana) tropicalis
Plasmid#75355PurposeNt His-tag Xenopus (Silurana) tropicalis Sacsin 87-1376DepositorInsertSacsin Xenopus (Silurana) tropicalis 87-1376
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pColdII-SIRPT1-Ceratotherium-simum-simum
Plasmid#78111PurposeNt His-tag Ceratotherium-simum-simum Sacsin 89-1366DepositorInsertSacsin Ceratotherium-simum-simum 89-1366
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pColdII-SIRPT1-Mustela putorius furo
Plasmid#78116PurposeNt His-tag Mustela putorius furo Sacsin 94-1381DepositorInsertSacsin Mustela putorius furo 94-1381
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP81xV5_ccdB
Plasmid#79019PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selctionDepositorTypeEmpty backboneUseTagsdouble V5 epitope tagExpressionYeastMutationPromoterAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP82xV5_ccdB
Plasmid#79020PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag.attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selectionDepositorTypeEmpty backboneUseTagsdouble V5 epitope tagExpressionYeastMutationPromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK241
Plasmid#71693PurposeProduces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Trp404>Phe mutant (AarAH6-W404F)DepositorInsertcitrate synthase
UseTagsHis6ExpressionBacterialMutationchanged Tro404 to PhePromoterT7 promoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only