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hgRNA-B21_pLKO-Hyg

Plasmid

#100560

Purpose

hgRNA-B21 in a lentiviral backbone

Depositor

George Church

Article

Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

Insert

hgRNA-B21

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDECKO_GFP

Plasmid

#141210

Purpose

empty GFP vector for cloning sgRNA or pgRNAs

Depositor

Rory Johnson

Article

Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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CLK1 gRNA (BRDN0001146512)

Plasmid

#76230

Purpose

3rd generation lentiviral gRNA plasmid targeting human CLK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CLK1 (CLK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CLK1 gRNA (BRDN0001147209)

Plasmid

#76231

Purpose

3rd generation lentiviral gRNA plasmid targeting human CLK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CLK1 (CLK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TL024_lenti_hSyn:HaloTag-GluA1

Plasmid

#192517

Purpose

Expression of AMPA GluA1 with N terminal HaloTag fusion

Depositor

Adam Cohen

Article

Kim et al bioRxiv. 2023 May 29:2023.05.26.541296.

Insert

HaloTag-GluA1

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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A*68:01:01

Plasmid

#203292

Purpose

Overexpress HLA allele

Depositor

Catherine Wu, Derin Keskin

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*68:01:01 (HLA-A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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mPERK

Plasmid

#24826

Depositor

Fumihiko Urano

Article

Ghosh et al PLoS One. 2010 . 5(3):e9575.

Insert

mPERK (Eif2ak3 Mouse)

Use

Lentiviral; Destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2010

Availability

Academic Institutions and Nonprofits only
pHAGE-ERBB2-L755_T759delLRENT

Plasmid

#116339

Purpose

Lentiviral expression of ERBB2 L755_T759delLRENT

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB2 (ERBB2 Human)

Use

Lentiviral

Tags

Expression

Mutation

L755_T759delLRENT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Flag Depdc5 pLJM1

Plasmid

#46336

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Differs from Depdc5 isoform 1 by 10 amino acids …

Promoter

Available since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
TFORF3168

Plasmid

#144644

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

FOXM1 (FOXM1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-W531C

Plasmid

#116210

Purpose

Lentiviral expression of BRAF W531C

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Tags

Expression

Mutation

W531C

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-T241P

Plasmid

#116197

Purpose

Lentiviral expression of BRAF T241P

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Tags

Expression

Mutation

T241P

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-T599I

Plasmid

#116201

Purpose

Lentiviral expression of BRAF T599I

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Tags

Expression

Mutation

T599I

Promoter

Available since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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TMED3-hLOV-TEVcs-GAL4bd-VP64-V5

Plasmid

#170996

Purpose

HiLITR transcription factor with full-length TMED3 targeting sequence (ER/Golgi)

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

TMED3(FL)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5 (TMED3 Synthetic)

Use

Lentiviral and Synthetic Biology

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ef1-alpha

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-NDC80

Plasmid

#31215

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

NDC80 (NDC80 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
pLV-tetO-Id2

Plasmid

#70764

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Id2

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

inhibitor of DNA binding 2 (Id2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only
pLVTHM-Bptf-sh28

Plasmid

#83278

Purpose

shRNA targeting BPTF

Depositor

Joe Landry

Article

Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

Insert

shRNA #28 to BPTF (BPTF, Bptf Mouse, Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE-H1

Available since

Oct. 25, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pLVTHM-Bptf-sh5

Plasmid

#83277

Purpose

shRNA targeting BPTF

Depositor

Joe Landry

Article

Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

Insert

shRNA #5 to BPTF (BPTF, Bptf Mouse, Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE-H1

Available since

Oct. 25, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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CSK gRNA (BRDN0001149085)

Plasmid

#77767

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CSK (CSK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSLIK JunD-HA neo

Plasmid

#58515

Purpose

Lentiviral expression vector for an inducible HA-tagged mouse JunD

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

JunD (Jund Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only