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Plasmid#58854PurposeConstitutive expression of tTA-EBFP fusion protein (tTA from CL-CTIG, Addgene plasmid 14901)DepositorInserttTA transcription factor fused to enhanced blue fluorescent protein
UseTagsBFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pI1-MTU-DO-URA
Plasmid#160009PurposeIntegrative vector for K. marxianus, targeting the LAC4 locus. Uracil selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-MYC-e3
Plasmid#120341PurposepGL4.23-MYC with an enhancer cloned upstream of the MYC promoter.DepositorInsertchr8:129056967-129058438 (hg19)
UseLuciferaseTagsExpressionMammalianMutationPromoterMYCAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
paavCAG-Jx-rev-post-mGRASP-2A-dTomato
Plasmid#34913PurposeJxON-postDepositorInsertpostsynaptic mGRASP
UseAAV and Cre/LoxTagsExpressionMutationPromoterCAGAvailable sinceNov. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cyclin E-pBI-EGFP
Plasmid#16654DepositorInsertCyclin E (CCNE1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-SpCAS9-NG-pA
Plasmid#131465PurposeExpresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized SpCas9-NG
UseCRISPRTagsFlag, NLS, and polyAExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB6574
Plasmid#106132PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
prCAG-BoxB
Plasmid#191122PurposeTranscription of 47xCAG and 12xBoxB repeats from a tetracycline-inducible promoterDepositorInsertsrCAG
BoxB
UseSynthetic BiologyTagsExpressionMutationPromotertetracycline-inducible promoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ144-ZmUbi-tRNA
Plasmid#158402PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ134-tRNA2.0; assembly of 4 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pI6-MTU-DO-URA
Plasmid#160030PurposeIntegrative vector for K. marxianus, targeting the ARO3 locus. Uracil selection. Low integration efficiency in an NHEJ-deficient background.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
paavCAG-JxON-pre-mGRASP-mCerulean
Plasmid#51903PurposeJxOn-preDepositorInsertpresynaptic mGRASP
UseAAV and Cre/LoxTagsExpressionMutationPromoterCAGAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination
Plasmid#40132PurposeDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).DepositorTypeEmpty backboneUseTagsFokI nuclease (ELD)ExpressionMammalianMutationPromoterCAGAvailable sinceAug. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pC0.053
Plasmid#119571PurposeLevel 0 part. PromoterDepositorInsertPnirA
UseSynthetic BiologyTagsExpressionMutationRBS* added (22 bp) upstream of ATG.PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGNW4
Plasmid#122088Purposesuicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cellsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination
Plasmid#40131PurposeDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).DepositorTypeEmpty backboneUseTagsFokI nuclease (KKR)ExpressionMammalianMutationPromoterCAGAvailable sinceAug. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
Golgi-targeted optoPLD
Plasmid#140058PurposeEncodes CRY2-mCherry-PLD-P2A-CIBN-Sialyltransferase(TM) for mammalian expressionDepositorInsertPhospholipase D
UseTagsCIBN, CRY2, P2A, Sialyltransferase(TM), and mCher…ExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-Rsn2
Plasmid#61686Purposebacterial expression of ranaspumin 2 as a His-tagged fusion proteinDepositorInsertRanaspumin-2
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-mClover3
Plasmid#133453PurposeExpresses SpyTag003-mClover3 (a green fluorescent protein) in bacterial cytoplasmDepositorInsertSpyTag003-mClover3
UseTagsGSGSGSGS linker and His6ExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH2.21_mCherry-MS2x6
Plasmid#205536PurposeExpress mCherry-MS2x6 (export tag consisting of 6 tandem repeats of MS2 stem-loop aptamer)DepositorInsertmCherry
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330S-5
Plasmid#58781PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only