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Showing: 8661 - 8680 of 20484 results

  1. pSMAD3-donor

    Plasmid
    #112311
    Purpose
    CRISPR donor plasmid to tag human transcription factor SMAD3 with GFP
    Depositor
    Kevin White
    Insert
    SMAD3 homology arms flanking EGFP-IRES-Neo cassette (SMAD3 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:15:67189762:C>T, rs11629568, rs8025774
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112311-plasmid-map-sequence-id-228463

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  4. pRDA52_Lenti.puro.U6_modified DR

    Plasmid
    #196723
    Purpose
    For cloning pairs of AsCas12a guides with two DRs that are as different as possible.
    Depositor
    Bernhard Schmierer
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196723-plasmid-map-sequence-id-389696

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  5. pCMV-BFP-U6-sgRNA

    Plasmid
    #196725
    Purpose
    Sortable sgRNA expression, Chen tracrRNA PMID: 24360272
    Depositor
    Bernhard Schmierer
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196725-plasmid-map-sequence-id-388067

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  6. LHZ1493

    Plasmid
    #222102
    Purpose
    Cloning backbone for sgRNA in Kluyveromyces marxianus. Expresses highly specific SlugCas9.
    Depositor
    Yongming Wang
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    222102-plasmid-map-sequence-id-441240

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  7. SGEP-shRLuc-308

    Plasmid
    #188667
    Purpose
    control shRNA
    Depositor
    Lewis Cantley
    Insert
    shRLuc
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188667-plasmid-map-sequence-id-375067

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  8. SGEP-shRLuc-802

    Plasmid
    #188668
    Purpose
    control shRNA
    Depositor
    Lewis Cantley
    Insert
    shRLuc
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188668-plasmid-map-sequence-id-375059

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  9. p-dCas9-JMJD2A-Hygro

    Plasmid
    #104410
    Purpose
    transient expression of dCas9-JMJD2A fusion protein
    Depositor
    Stephan Beck
    Insert
    JMJD2A (KDM4A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104410-plasmid-map-sequence-id-204464

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  10. p-dCas9-G9a-Hygro

    Plasmid
    #104408
    Purpose
    transient expression of dCas9-G9a fusion protein
    Depositor
    Stephan Beck
    Insert
    G9a (EHMT2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104408-plasmid-map-sequence-id-204469

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  16. AAVS1-DRGFP

    Plasmid
    #113193
    Purpose
    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Insert
    DR-GFP
    Use
    Donor construct with t2a-hygromycin for gene trap…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113193-plasmid-map-sequence-id-216317

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  18. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1

    Plasmid
    #118157
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118157-plasmid-map-sequence-id-240364

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Showing: 8661 - 8680 of 20484 results