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Plasmid#104806PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr2g101090 (Pen3). Also expresses Cas9 from AtUBQ10 promoter.DepositorInsertMedtr2g101090
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC31
Plasmid#104805PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr2g101090 (Pen3). Also expresses Cas9 from Gmubi promoter.DepositorInsertMedtr2g101090
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC27
Plasmid#104801PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr4g020620 (Pho2ab). Also expresses Cas9 from Gmubi promoter.DepositorInsertMedtr4g020620
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC26
Plasmid#104800PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr2g101040 (Mel1). Also expresses Cas9 from AtUBQ10 promoter.DepositorInsertMedtr2g101040
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC20
Plasmid#104794PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr2g020630 (FmoI). Also expresses Cas9 from AtUBQ10 promoterDepositorInsertMedtr2g020630
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC14
Plasmid#104788PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr2g044580 (ERDJ2). Also expresses Cas9 from AtUBQ10 promoterDepositorInsertMedtr2g044580
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC15
Plasmid#104789PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr2g044580 (ERDJ2). Also expresses Cas9 from rolD promoterDepositorInsertMedtr2g044580
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC13
Plasmid#104787PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr2g044580 (ERDJ2). Also expresses Cas9 from Gmubi promoterDepositorInsertMedtr2g044580
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_HOMEZ
Plasmid#86289PurposeDonor vector for 3' FLAG tag of human HOMEZDepositorInsertHOMEZ homology arms (HOMEZ Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_HBP1
Plasmid#86272PurposeDonor vector for 3' FLAG tag of human HBP1DepositorInsertHBP1 homology arms (HBP1 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJZC103
Plasmid#62340PurposesgRNA for mammalian cells with mCherry markerDepositorInsertsgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-SYNJ2BP-1
Plasmid#92159PurposeCRISPR guide RNA targeting human SYNJ2BPDepositorInsertSYNJ2BP sgRNA-1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceAvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-SYNJ2BP-2
Plasmid#92160PurposeCRISPR guide RNA targeting human SYNJ2BPDepositorInsertSYNJ2BP sgRNA-2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pLX_TRC311
Plasmid#113668PurposeGateway destination vector for lentiviral expressionDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTCyan
Plasmid#85551PurposeInducible expression of guide with fluorescent Cyan reporterDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterH1tAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTCherry
Plasmid#85552PurposeInducible expression of guide with fluorescent Cherry reporterDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterH1tAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-EBFP2-NLS (pRW1447)
Plasmid#216219PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expressionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR
Plasmid#118154PurposeCatalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.DepositorInsertKRAB-dCas9-P2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationSee depositor comments belowPromoterEF1a core and U6Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMYT095
Plasmid#198866PurposeCRISPR/Cas9 Vector with URA3 selectable marker to be used in the Ellis MYT systemDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Construct 11 - U6p_70nt_stgRNA
Plasmid#81250PurposeExpresses 70nt stgRNADepositorInsertsgRNA
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only