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Addgene
Showing: 8621 - 8640 of 16034 results

  10. Derp2.pJL1

    Plasmid
    #199116
    Purpose
    In vitro expression of Der p 2, a major common allergen from Dermatophagoides pteronyssinus (dust mite), with synthetic site for glycosylation encoded (GGNWTT)
    Depositor
    Michael Jewett
    Article
    Thames et al ACS Synth Biol. 2023 Apr 11. doi: 10.102
    Insert
    Der p 2 dust mite allergen
    Use
    Tags
    Glyctag, His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    199116-plasmid-map-sequence-id-390794

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  12. pHIT342

    Plasmid
    #204514
    Purpose
    Bacterial expression of SYP-1 N-terminal fragment with 6xHis and maltose binding protein tags for use as an antigen
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    N-terminal fragment of syp-1 (C. elegans)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Availability
    Academic Institutions and Nonprofits only
    204514-plasmid-map-sequence-id-403743

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  13. pHIT348

    Plasmid
    #204515
    Purpose
    Bacterial expression of CEC-5 C-terminal fragment with 6xHis and maltose binding protein tags for use as an antigen
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    C-terminal fragment of cec-5 (C. elegans)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Availability
    Academic Institutions and Nonprofits only
    204515-plasmid-map-sequence-id-411110

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  14. pHIT334

    Plasmid
    #204516
    Purpose
    Bacterial expression of COH-3 C-terminal fragment with 6xHis and maltose binding protein tags for use as an antigen
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    C-terminal fragment of coh-3 (C. elegans)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Availability
    Academic Institutions and Nonprofits only
    204516-plasmid-map-sequence-id-403820

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  15. pHIT521

    Plasmid
    #204520
    Purpose
    Bacterial expression of CEC-5 N-terminal fragment with 6xHis and maltose binding protein tags for in vitro assays
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    N-terminal fragment of cec-5 (C. elegans)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Availability
    Academic Institutions and Nonprofits only
    204520-plasmid-map-sequence-id-403749

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  16. pETM11-6xHis-TEV-hORF1p_StammerAEA

    Plasmid
    #202588
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Use
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Methionine 91 to Alanine, changed O…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    202588-plasmid-map-sequence-id-415632

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  20. Stx1a-12aa-mCer

    Plasmid
    #53236
    Purpose
    Fluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.
    Depositor
    Robert Zucker
    Article
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Insert
    Syntaxin 1A (Stx1a Rat)
    Use
    Tags
    12aa-linker and mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 8621 - 8640 of 16034 results