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pU6-sgGAL4-4

Plasmid

#46916

Purpose

Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter (negative control)

Depositor

Jonathan Weissman, Stanley Qi

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

sgGAL4-4
Puromycin resistance and mCherry

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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LeGO-Cer

Plasmid

#27351

Depositor

Boris Fehse

Article

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

U6 promoter, SFFV promoter, Cerulean

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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pCF525-AcrIIA5_gb36_lenti

Plasmid

#138306

Purpose

Lenti expression of AcrIIA5

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA5

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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L-CRISPR-CTN (MLL-ENL)

Plasmid

#69212

Purpose

Lentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; MLL-ENL,(t[11;19])

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-mNeonGreen

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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plko.1/shMCT4 477

Plasmid

#99598

Purpose

Knockdown of MCT4 expression

Depositor

Eli Bar

Article

Lim et al Oncogene. 2014 Aug 28;33(35):4433-41. do

Insert

MCT4 (SLC16A3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pLN380 (FuGW-S(CEBPB)p-mKate2)

Plasmid

#105178

Purpose

synthetic promoter CEBPB drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(CEBPB)p-mKate2

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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NLS-FUS-Citrine-PixE

Plasmid

#111506

Purpose

For the nuclear localization of the PixELL system

Depositor

Jared Toettcher

Article

Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

Insert

PixE (slr1693 )

Use

Lentiviral

Tags

Citrine, FUS, and NLS

Expression

Mutation

Promoter

SFFV

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pLX302-MKP7-V5 puro

Plasmid

#87771

Purpose

Expresses V5 tagged human MAPK phosphatase 7 (MKP7/DUSP16)

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKP7 (DUSP16 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSicoR-mCh-Oct4i

Plasmid

#21906

Depositor

Miguel Ramalho-Santos

Article

Gaspar-Maia et al Nature. 2009 Jul 8. ():.

Insert

Oct4i

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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pBoBi-hLAMP2-C-PEN2

Plasmid

#183646

Purpose

Lentiviral expression of a lysosome-residential PEN2.

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 and LAMP2 (PSENEN, LAMP2 Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

WT

Promoter

CMV

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLp6.3-Reverse

Plasmid

#79117

Purpose

Lentiviral expression of EglN3-Reverse

Depositor

Susanne Schlisio

Insert

control plasmid with reverse EglN3 insert (EGLN3 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-Macro-Linker-eGFP

Plasmid

#176067

Purpose

EGFP fused to the C-terminus of a Macrodomain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

Macro

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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LCV2_mClover3_AAVS1_sgRNA_2

Plasmid

#155099

Purpose

lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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tet-pLKO.puro_shHuR 3UTR

Plasmid

#110412

Purpose

TRCN0000276186 (Target TTGTTAGTGTACAACTCATTT), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selection

Depositor

Sandra Martha Gomes Dias

Insert

ELAVL1 (HuR)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1/TO (RNA PolIII)

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide1

Plasmid

#118162

Purpose

CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the EGFP CDS

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

KRAB-dCas9-2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

hPGK and U6

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Elf5

Plasmid

#70772

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Elf5

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Elf5 (Elf5 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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TFORF2821

Plasmid

#143196

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

IKZF5 (IKZF5 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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dCas12j3 VPR

Plasmid

#188517

Purpose

Expresses FLAG tagged dCas12j3 VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12j3

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D413A

Promoter

EF1a

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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dCas12j3 KRAB

Plasmid

#188507

Purpose

Expresses FLAG tagged dCas12j3 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12j3

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D413A

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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dCas12j2 VPR

Plasmid

#188518

Purpose

Expresses FLAG tagged dCas12j2 VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12j2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D394A

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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