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pCMV-SlugCas9-P2A-puro

Plasmid

#214353

Purpose

Expresses SlugCas9 and puromycin resistance genes

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SlugCas9 and puromycin resistance genes

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-4V5-Pho8-Met

Plasmid

#207029

Purpose

Expression of 4V5-Pho8 under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Pho8

Use

Tags

4xV5

Expression

Yeast

Mutation

Promoter

pCUP1

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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BS-Trp-PCUP1-4myc-Pho8

Plasmid

#207028

Purpose

Expression of 4Myc-Pho8 under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Pho8

Use

Tags

4xMyc

Expression

Yeast

Mutation

Promoter

pCUP1

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-Vac8-GFP-Ura

Plasmid

#207027

Purpose

Expression of Vac8-GFP under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Vac8

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

pCUP1

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-GFP-Vma13-Ura

Plasmid

#207026

Purpose

Expression of GFP-Vma13 under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Vma13 ORF

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

pCUP1

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pFB-MBD2.4Ch (pc2393)

Plasmid

#211730

Purpose

Expresses MBD2 (aa236-414) mCherry tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2.4 aa236-414

Use

Tags

mCherry

Expression

Insect

Mutation

Promoter

Polyhedrin promotor

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pFB-MBD2.3Ch (pc2392)

Plasmid

#211729

Purpose

Expresses MBD2 (aa222-414) mCherry tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2.3 aa222-414

Use

Tags

mCherry

Expression

Insect

Mutation

Promoter

Polyhedrin promotor

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pFB-MBD2.5R (pc2395)

Plasmid

#211727

Purpose

Expresses MBD2 (aa153-221) RFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2.5 aa153-221

Use

Tags

RFP

Expression

Insect

Mutation

Promoter

Polyhedrin promotor

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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P1K-3HA-Atg8∆GR-MET15

Plasmid

#207033

Purpose

Expression of 3HA-Atg8∆GR. This variant cannot be conjugated to PE.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Use

Tags

3xHA

Expression

Yeast

Mutation

∆GR

Promoter

pATG8

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pFB-MBD2.6R (pc2397)

Plasmid

#211725

Purpose

Expresses MBD2 (aa153-235) RFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2.6 aa153-235

Use

Tags

RFP

Expression

Insect

Mutation

Promoter

Polyhedrin promotor

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFB-MBD2.1mR (pc2391)

Plasmid

#211723

Purpose

Expresses MBD2 (aa1-152) RFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2.1 aa1-152

Use

Tags

RFP

Expression

Insect

Mutation

Promoter

Polyhedrin promotor

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFB_MBD2.2-GFP (pc2083)

Plasmid

#211722

Purpose

Expresses MBD2 (aa153-414) GFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2.2 aa153-414

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

Polyhedrin Promoter

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFB_MBD2.1-GFP (pc2082)

Plasmid

#211721

Purpose

Expresses MBD2 (aa1-152) GFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2 aa1-152

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

Polyhedrin Promoter

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFB-MeCP2.9Ch (pc2385)

Plasmid

#211720

Purpose

Expresses MeCP2 (aa163-309) mCherry tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MeCP2 aa163-309

Use

Tags

mCherry

Expression

Insect

Mutation

Promoter

Polyhedrin Promoter

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFB-MeCP2G.9 (pc1574)

Plasmid

#211719

Purpose

Expresses MeCP2 (aa163-309) GFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MeCP2 aa163-309

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

Polyhedrin Promoter

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFB-MeCP2G.8 (pc1573)

Plasmid

#211718

Purpose

Expresses MeCP2 (aa310-492) GFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MeCP2 aa310-492

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

Polyhedrin Promoter

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFB MBD2a-2-strep (pc1794)

Plasmid

#211715

Purpose

Expresses fl. MBD2 2xStrep tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2

Use

Tags

2xStrep

Expression

Insect

Mutation

Promoter

Polyhedrin Promoter

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor_RB-TnV2_pJEx

Plasmid

#213908

Purpose

Barcoded mariner transposon with crystal violet-inducible outward promoter - barcodes can be added via FseI-SbfI restriction sites

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with kan resistance and EilR/pJEx and FseI-SbfI sites for barcode insertion

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-mRuby3-Pho8-406

Plasmid

#207043

Purpose

Expression of mRuby3-Pho8 under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

PHO8

Use

Tags

mRuby3

Expression

Yeast

Mutation

Promoter

pCUP1

Available since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pFB MBD2a-C-GFP (pc1565)

Plasmid

#211712

Purpose

Expresses fl. MBD2 GFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

Polyhedrin Promoter

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge