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pCAGGS-AcGFP-C

Plasmid

#203325

Purpose

Express N-terminal AcGFP-fused protein in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Use

Tags

AcGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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Human USP30 (1-517, C77A, MG-38-16)

Plasmid

#110749

Purpose

Transient transfection of human USP30 C77A

Depositor

David Komander

Article

Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

Insert

USP30 (USP30 Human)

Use

Tags

Expression

Mammalian

Mutation

C77A

Promoter

CMV

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBbA2c-RFP

Plasmid

#35326

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Tet

Available since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pJUMP48-2A(sfGFP)

Plasmid

#126993

Purpose

Level 2 vector. sfGFP reporter. Origin pUC (high copy number)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pBbB6k-GFP

Plasmid

#35357

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

LlacO-1

Available since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pFRETgc-2in1-NN

Plasmid

#105124

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Use

Tags

mGFP/mCherry

Expression

Plant

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-5X2-RalBP1

Plasmid

#37245

Depositor

Christopher Counter, David Kashatus

Article

Kashatus et al Nat Cell Biol. 2011 Aug 7;13(9):1108-15.

Insert

RalBP1 (RALBP1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pCAG-hHDAC6

Plasmid

#21099

Depositor

Connie Cepko

Article

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

Histone Deacetylase 6 (HDAC6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
pBRIT Pax7 FLAG

Plasmid

#17521

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pSMP-Luc

Plasmid

#36394

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Firefly Luciferase

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
mNP-Luc (PPARdelta promoter)

Plasmid

#16532

Depositor

Bert Vogelstein

Article

He et al Cell. 1999 Oct 29. 99(3):335-45.

Insert

PPAR delta promoter NP (PPARD Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant binding sites

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pJFRC150-20XUAS-IVS-Flp1::PEST

Plasmid

#32132

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

Flippase1::PEST

Use

Tags

PEST

Expression

Insect

Mutation

Aspartic Acid at Amino Acid 5

Promoter

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pJYDN2

Plasmid

#162454

Purpose

Yeast display plasmid intended for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA) and expression of N-terminal fusions with Aga2p yeast agglutinin.

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Type

Empty backbone

Use

Shuttle vector bacteria/yeast

Tags

DnbALFA nanobody and Ha and myc

Expression

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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phGR-SB

Plasmid

#133409

Purpose

overexpression of a human glucocorticoid receptor in mammalian cells

Depositor

Andrew Cato

Article

Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

Insert

NR3C1 (NR3C1 Human)

Use

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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EcRho

Plasmid

#113121

Purpose

Expresses E. coli Rho

Depositor

James Berger

Article

Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

Insert

Rho

Use

Tags

MGH

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pKeratin-PSmOrange

Plasmid

#31924

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Use

Tags

Keratin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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HA GST PreScission p70 S6K1

Plasmid

#15511

Depositor

David Sabatini

Article

Sancak et al Mol Cell. 2007 Mar 23. 25(6):903-15.

Insert

p70 S6K1 (Rps6kb1 Rat)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pBbA8c-RFP

Plasmid

#35272

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

BAD

Available since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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CAG-RbpjEnR

Plasmid

#160924

Purpose

dominant-negative construct to inhibit Notch signaling

Depositor

Mark Emerson

Article

Chen et al Sci Rep. 2021 Jul 15;11(1):14525. doi: 1

Insert

RbpjEnR

Use

Tags

Expression

Mutation

fused with an engrailed repressor

Promoter

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-mWnt7b

Plasmid

#42283

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt7b (Wnt7b Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only