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pPPC030.306

Plasmid

#171151

Purpose

Mevalonate production pathway with J306 scRNA on pBBR1-GmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Inserts

J306 scRNA
J3-BBa_J23117-mvaES

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119 and J3-BBa_J23117

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-null (BPK1077)

Plasmid

#171128

Purpose

Plasmid encoding a pCMV-T7 with a 2 amino acid peptide prior to a stop codon.

Depositor

Benjamin Kleinstiver

Article

Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

Insert

pCMV-T7 null expression plasmid that encodes a two amino acid peptide (Met-Asp)

Use

CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

pCMV and T7

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pVMG0217_Cq-U6-2b_2xBbsI-gRNA

Plasmid

#169339

Purpose

Expression of gRNA in Culex quinquefasciatus or other mosquitoes

Depositor

Valentino Gantz

Article

Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

Insert

pVMG0217_Cq-U6-2b_2xBbsI-gRNA

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

CPIJ039728

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCBH-Cas9-Suntag-BB_BbsI

Plasmid

#164805

Purpose

REDIT backbone for SunTag Cas9, pU6-gRNA-backbone(BbsI)-CBH-SpCas9-Suntag-T2A-EBFP

Depositor

Le Cong

Article

Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pJK528

Plasmid

#155388

Purpose

dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs

Use

CRISPR

Tags

Expression

Bacterial

Mutation

D917A (nuclease-deactivating)

Promoter

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pJK434

Plasmid

#155378

Purpose

PphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas9 (bacteria)
PA4-mVenus
PphlF_A4NT in dCas9 sgRNA oscillator v1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Promoter

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pJK422

Plasmid

#155373

Purpose

dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 412; pOSIP)

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas9 (bacteria)
PA4-mVenus
dCas9 sgRNA oscillator v1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Promoter

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pJK423

Plasmid

#155374

Purpose

dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 413; pOSIP)

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas9 (bacteria)
PA4-mVenus
dCas9 sgRNA oscillator v1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Promoter

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10AC1)_Psyn-sgRNArodA

Plasmid

#149659

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30(-10AC1), Psyn

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10AC2)_Psyn-sgRNArodA

Plasmid

#149660

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30(-10AC2), Psyn

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNAftsI

Plasmid

#149591

Purpose

all-in-all CRISPRi vector for targeting B. burgdorferi ftsI

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAftsI

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30(-10TC), Psyn

Available since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNArodA

Plasmid

#149657

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30(-10TC), Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pFETCh_ELK1_iso1

Plasmid

#135737

Purpose

Donor vector for 3' FLAG tag of human ELK1_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ELK1_iso1 Homology arms (ELK1 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pFETCh_MAF1_iso1

Plasmid

#135732

Purpose

Donor vector for 3' FLAG tag of human MAF1_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

MAF1_iso1 Homology arms (MAF1 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pFB-U6-control-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128347

Purpose

pAAV encoding control gRNA for CRISPR gRNAs listed above

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

control (negative)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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gh161

Plasmid

#106825

Purpose

expression of gRNA targeting TMTC2

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

TMTC2 (TMTC2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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gh89

Plasmid

#106760

Purpose

expression of gRNA targeting FUT5

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

FUT5 (FUT5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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gh144

Plasmid

#106803

Purpose

expression of gRNA targeting LARGE

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

LARGE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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gh148

Plasmid

#106818

Purpose

expression of gRNA targeting KDELC2

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

KDELC2 (POGLUT3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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gh104

Plasmid

#106774

Purpose

expression of gRNA targeting XXYLT1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

XXYLT1 (XXYLT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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