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pMXs-hs-NRAS

Plasmid

#52728

Purpose

retroviral expression of human NRAS

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

NRAS (NRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only
pCIneoLuc-PP1A

Plasmid

#128348

Purpose

Vector for LUMIER assay

Depositor

Yutaka Hata

Article

Kawano et al J Biochem. 2015 May 14. pii: mvv051.

Insert

PP1A (PPP1CA Human)

Use

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-QUASM1:mScarletNLS-SV40pA

Plasmid

#155124

Purpose

Tol2 vector containing QUASM1 reporter element upstream of mScarlet-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1:mScarletNLS

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

QUASM1-E1b

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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MO70-hJunctate

Plasmid

#79596

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Proc Natl Acad Sci U S A. 2012 May 29;10

Insert

junctate (ASPH Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only
pMT2 TIA1 RRM-CFP-FRBx5

Plasmid

#103783

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

TIA1 RRM-CFP-FRBx5

Use

Tags

CFP

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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Sha2Cas9-HF

Plasmid

#192142

Purpose

Expresses highly specific Sha2Cas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

Insert

Sha2Cas9-HF

Use

AAV

Tags

Expression

Mammalian

Mutation

Sha2Cas9-HF (R247A and N415A)

Promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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YCplac33-NAT_MCART1

Plasmid

#140591

Purpose

Express MCART1 in S. cerevisiae

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

SLC25A51 with 5' and 3' UTR of scNDT1 (SLC25A51 Human)

Use

Tags

Expression

Yeast

Mutation

codon-optimized for expression in S. cerevisiae

Promoter

NDT1 promoter and 3'UTR

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-H2-Ld

Plasmid

#135519

Purpose

Mammalian expression of myc-tagged H2-Ld

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

H2-Ld

Use

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGB3_Omega2_Pnos:GR:LacIBD:Gal4AD:Tnos-OplacI:mini35S:RDF:Tnos (GB1678)

Plasmid

#160642

Purpose

Module for the dexamethasone -inducible expression of PhiC31 phage recombination directionality factor (RDF) gene.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

GRLacIBDGal4AD / RDF

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-ms-c-Myc-V394D

Plasmid

#50775

Purpose

retroviral expression of mouse c-Myc V394D mutant

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Jul 26. (

Insert

c-Myc V394D (Myc Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

V394D (does not bind to Miz-1)

Promoter

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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SSpB-mCherry-p63DH

Plasmid

#176112

Purpose

Heterodimerization with iLID, RhoA activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

RHOGEF p63 (ARHGEF25 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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PIK3CA - Exon9 E545K (pFastBac)

Plasmid

#16640

Depositor

Bert Vogelstein

Article

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA E545K (PIK3CA Human)

Use

Baculoviral

Tags

His

Expression

Mammalian

Mutation

E545K

Promoter

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
KG#203

Plasmid

#110880

Purpose

Expresses the C. elegans pde-4 (isoform d) cDNA pan-neuronally

Depositor

Kenneth Miller

Article

Charlie et al Genetics. 2006 May;173(1):111-30. doi: 1

Inserts

rab-3 promoter
pde-4d (isoform d) cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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ins:BB1.0L; cryaa:RFP

Plasmid

#110283

Purpose

A zebrafish insulin promoter driving the Brainbow (BB1.0L) cassette. Contains red eye marker, and is flanked with I-SceI sites.

Depositor

Nikolay Ninov

Article

Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

Insert

Brainbow1.0L

Use

Zebrafish

Tags

Expression

Mutation

Promoter

insulin

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-A774L

Plasmid

#174796

Purpose

Bacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-A774L (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

A774L, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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dmBACCS2VL-IRES-dOrai-IRES-mCherry

Plasmid

#72898

Purpose

Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2VL-IRES-dOrai-IRES-mCherry

Use

Tags

IRES-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-TFAP4(E135A/S139A)

Plasmid

#110157

Purpose

Expression of human TFAP4 (E135A/S139A) in mammalian cells

Depositor

Daniele Guardavaccaro

Article

D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

Insert

TFAP4 (transcription factor AP-4) (TFAP4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

E135A/S139A

Promoter

CMV

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-p97-AE-mycStrep

Plasmid

#31841

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Use

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

A232E

Promoter

CMV

Available since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3 PARL-FLAG-CT S65D+T69D+S70D

Plasmid

#13617

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 65, Thr 69 and Ser70 to Asp

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pLV.U6.gLacZ.Cas9-T2A-GFP

Plasmid

#170361

Purpose

Negative control plasmid used for Crispr/cas9 based disruption.

Depositor

Johan Jakobsson

Article

Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

Insert

Cas9-T2A-GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

June 27, 2021

Availability

Academic Institutions and Nonprofits only

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