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pLentiCrisprV2 sgRNA hPER2c-term #2

Plasmid

#179455

Purpose

Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

sgRNA targeting human PER2

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCrisprV2 sgRNA hPER2c-term #3

Plasmid

#179456

Purpose

Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

sgRNA targeting human PER2

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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p1194-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIA4_FLAG_NLS

Plasmid

#129532

Purpose

Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIA4

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Inserts

codon-optimized AcrIIA4
sgRNA_Rosa26

Use

AAV

Tags

FLAG/NLS

Expression

Mutation

Promoter

U6 and cytomegalovirus-enhancer chicken β-actin p…

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCrisprV2 sgRNA hCRY1 c-term

Plasmid

#179453

Purpose

Lentiviral Crispr/Cas9 plasmid targeting hCRY1 at C-terminus to generate C-terminal knock-in

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

sgRNA targeting human CRY1

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-sgRNA2-RPB1-N-term

Plasmid

#195111

Purpose

Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

sgRNA against first exon of RPB1 (N-terminal)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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lenti-sgRNA(MS2)_zeo_hCYP3A4_enhancer_guide1

Plasmid

#176839

Purpose

To recruit SAM complex to human CYP3A4 proximal enhancer.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

Human CYP3A4 proximal enhancer sgRNA for activation

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-PpU6P-sgRNA-L1L2

Plasmid

#113735

Purpose

Gateway entry vector containing attL1 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR; Gateway entry vector

Tags

Expression

Mutation

Promoter

P. patens U6 promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro G3BP1-sgRNA

Plasmid

#196197

Purpose

Cas9 + gRNA expression to edit G3BP1 locus

Depositor

Jürgen Knoblich

Article

Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

Insert

G3BP1 (G3BP1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9-Puro HLAG-2A-sgRNA

Plasmid

#182551

Purpose

Cas9 from S.pyogenes with 2A-Puro, and the 2A-sgRNA to targeting exon 2 of human HLA-G gene

Depositor

Santiago Miriuka

Article

Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

Insert

hSpCas9-2A-Puro-HLAG-2A-sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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Expression scaffold for dCas9+sgRNA

Plasmid

#171799

Purpose

PICASSO: Expression scaffold for second subcloning step to properly co-express paired dCas9-fusion+sgRNA

Depositor

Stephen Elledge

Article

Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

Insert

PICASSO: expression scaffold for proper dCas9-fusion + sgRNA expression

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-sgRNA2-2-RPB1-C-term

Plasmid

#195109

Purpose

Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 C-term. Puromycin selection (2A-fusion).

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

sgRNA against last exon of RPB1 (C-terminal)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRNA-Cre-GpNLuc

Plasmid

#208382

Purpose

Derived from LentiCRISPRV2 by replacing Cas9 with Cre recombinase and puromycin resistance with GpNLuc. Contains a stuffer sequence for cloning of sgRNA of interest, as per LentiCRISPRV2.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Type

Empty backbone

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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SauCas9 EMX1-nicking sgRNA

Plasmid

#169860

Purpose

SauCas9 nicking sgRNA for EMX1

Depositor

Scot Wolfe

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SauCas9 EMX1-nicking sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6 promoter

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pMS79 (eft-3p::Cas9 + sgRNA)

Plasmid

#154839

Purpose

Cas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGG

Depositor

Patrick Phillips

Article

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

sgRNA

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-ACTB_sgRNA

Plasmid

#183884

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ACTB sgRNA spacer (ACTB Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-TUBA1B_sgRNA

Plasmid

#183888

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

TUBA1B sgRNA spacer (TUBA1B Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-RHOA_sgRNA

Plasmid

#183877

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and RHOA sgRNA for N-terminal tagging of RhoA in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

RHOA sgRNA spacer (RHOA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA1-PP7-boxB

Plasmid

#75393

Purpose

sgRNA1-PP7-boxB

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-2XPP7-boxB

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMD19-PBBa_J23117-sgRNA-Spr

Plasmid

#190793

Purpose

Template vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.

Depositor

Paul Hudson

Article

Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

Insert

sgRNA (dummy)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23117

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS

Plasmid

#129530

Purpose

Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Inserts

codon-optimized AcrIIC3Nme
sgRNA_Rosa26

Use

AAV

Tags

FLAG/NLS

Expression

Mutation

Promoter

U6 and cytomegalovirus-enhancer chicken β-actin p…

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge